Functional Diversity of Proteinases Encoded by Genes of the Rat Tissue Kallikrein Family

1992 ◽  
pp. 42-50
Author(s):  
F. Gauthier ◽  
T. Moreau ◽  
N. Gutman ◽  
A. El Moujahed ◽  
M. Brillard-Bourdet
Keyword(s):  
Functional Diversity ◽  
Tissue Kallikrein ◽  
Rat Tissue

scholarly journals Immunocytochemical localization of tissue kallikrein in brain ventricular epithelium and hypothalamic cell bodies.

1985 ◽  
Vol 33 (9) ◽  
pp. 951-953 ◽  
Author(s):  
J A Simson ◽  
R Dom ◽  
J Chao ◽  
C Woodley ◽  
L Chao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Epithelial Cells ◽  
Third Ventricle ◽  
Immunoperoxidase Staining ◽  
Tissue Kallikrein ◽  
Immunocytochemical Localization ◽  
Specific Monoclonal Antibody ◽  
The Third ◽  
Indirect Immunoperoxidase ◽  
Rat Tissue

A specific monoclonal antibody against rat tissue kallikrein was used as the primary antibody for indirect immunoperoxidase staining of rat hypothalamus. Kallikrein was localized in the epithelial cells (ependyma) lining the third ventricle as well as in cell bodies of arcuate, supraoptic, paraventricular, and ventromedial nuclei.

Physical Mapping of the Rat Tissue Kallikrein Family in Two Gene Clusters by Analysis of P1 Bacteriophage Clones

Genomics ◽  
1994 ◽  
Vol 22 (2) ◽  
pp. 404-417 ◽  
Author(s):  
Michelle Southard-Smith ◽  
James C. Pierce ◽  
Raymond J. MacDonald
Keyword(s):  
Physical Mapping ◽  
Gene Clusters ◽  
Tissue Kallikrein ◽  
Rat Tissue

A Monoclonal Antibody to Rat Tissue Kallikrein: Use in Biochemical and Immunohistochemical Studies

1986 ◽  
pp. 503-513 ◽  
Author(s):  
Cheryl M. Woodley ◽  
Julie Chao ◽  
Jo Ann Simson ◽  
Harry S. Margolius ◽  
Lee Chao
Keyword(s):  
Monoclonal Antibody ◽  
Tissue Kallikrein ◽  
Rat Tissue ◽  
Immunohistochemical Studies

Inhibition of rat tissue kallikrein gene family members by rat kallikrein-binding protein and α1-proteinase inhibitor

FEBS Letters ◽  
1992 ◽  
Vol 309 (3) ◽  
pp. 405-408 ◽  
Author(s):  
C. Serveau ◽  
T. Moreau ◽  
G.X. Zhou ◽  
J. Chao ◽  
F. Gauthier
Keyword(s):  
Gene Family ◽  
Proteinase Inhibitor ◽  
Binding Protein ◽  
Family Members ◽  
Tissue Kallikrein ◽  
Rat Tissue

Competitive Parabolic Inhibition of Rat Tissue Kallikrein by Aprotinin

2009 ◽  
Vol 5 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Carolina Diniz ◽  
Luciana Xavier ◽  
Marcelo Santoro ◽  
Amintas Figueiredo
Keyword(s):  
Tissue Kallikrein ◽  
Rat Tissue

A 1H-NMR study of the interactions between rat tissue kallikrein and two peptide inhibitors

1992 ◽  
Vol 1160 (2) ◽  
pp. 235-238 ◽  
Author(s):  
Talal S. El-Thaher ◽  
Graham S. Bailey ◽  
Michael T. Wilson ◽  
Michael Osborne ◽  
Geoffrey R. Moore
Keyword(s):  
1H Nmr ◽  
Peptide Inhibitors ◽  
Tissue Kallikrein ◽  
Nmr Study ◽  
Rat Tissue

scholarly journals Tissue kallikrein synthesis and its modification by testosterone or low dietary sodium

1984 ◽  
Vol 218 (1) ◽  
pp. 37-43 ◽  
Author(s):  
D H Miller ◽  
J Chao ◽  
H S Margolius
Keyword(s):  
Total Protein ◽  
Relative Rate ◽  
Specific Radioactivity ◽  
Submaxillary Gland ◽  
Enzyme Synthesis ◽  
Dietary Sodium ◽  
Tissue Kallikrein ◽  
Comparable Effect ◽  
Descending Colon ◽  
Rat Tissue

A method has been developed to measure the relative rate of rat tissue kallikrein synthesis which employs a specific antiserum raised against a purified rat urinary kallikrein. Incorporation of [35S]methionine into kallikrein and protein 20 min after intraperitoneal injection was measured in submaxillary gland, pancreas, kidney and descending colon. Kallikrein content was measured with a direct radioimmunoassay, and kallikrein-specific incorporation of [35S]methionine measured after immunoprecipitation. Kallikrein specific radioactivity (c.p.m./mg of enzyme) was about 100-fold greater than that in total protein in both kidney and colon. In contrast, in pancreas the incorporation into the enzyme was only 5-fold higher than into protein, and in submaxillary gland the incorporation was equivalent. Measured as kallikrein-specific radioactivity relative to total protein radioactivity incorporated in 20 min, kallikrein represents 0.18% of total protein synthesis in the kidney, 0.34% in the pancreas, 0.41% in the colon, but 7.29% in the submaxillary gland. Dietary Na+ restriction increased the relative rate of kallikrein synthesis 1.8-fold in the kidney without a comparable effect in submaxillary gland. In contrast, testosterone increased the relative rate of synthesis 2.3-fold in submaxillary gland, but decreased it in kidney. The data show that endogenous kallikrein synthesis differs markedly in various tissues, and that interventions which are known to change kallikrein content or excretion also change the relative rate of enzyme synthesis.

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