scholarly journals Immunocytochemical localization of tissue kallikrein in brain ventricular epithelium and hypothalamic cell bodies.

1985 ◽  
Vol 33 (9) ◽  
pp. 951-953 ◽  
Author(s):  
J A Simson ◽  
R Dom ◽  
J Chao ◽  
C Woodley ◽  
L Chao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Epithelial Cells ◽  
Third Ventricle ◽  
Immunoperoxidase Staining ◽  
Tissue Kallikrein ◽  
Immunocytochemical Localization ◽  
Specific Monoclonal Antibody ◽  
The Third ◽  
Indirect Immunoperoxidase ◽  
Rat Tissue

A specific monoclonal antibody against rat tissue kallikrein was used as the primary antibody for indirect immunoperoxidase staining of rat hypothalamus. Kallikrein was localized in the epithelial cells (ependyma) lining the third ventricle as well as in cell bodies of arcuate, supraoptic, paraventricular, and ventromedial nuclei.

scholarly journals Characterization of a monoclonal antibody (SBU-1) made to the thymic rudiment of sheep.

1985 ◽  
Vol 33 (8) ◽  
pp. 785-790 ◽  
Author(s):  
T R White ◽  
C S Lee ◽  
P French ◽  
D Hewish ◽  
M R Brandon
Keyword(s):  
Monoclonal Antibody ◽  
Epithelial Cells ◽  
Immunoperoxidase Technique ◽  
Fetal Sheep ◽  
Wool Fibers ◽  
Indirect Immunoperoxidase ◽  
Low Sulfur ◽  
Alpha Keratin ◽  
Keratinized Epithelium

A monoclonal antibody (SBU-1) was raised to sheep thymic rudiment by fusion of NSI myeloma cells with spleen cells from BALB/c mice immunized with thymic rudiment isolated from fetal sheep between 25-30 days of gestation. By employing the indirect immunoperoxidase technique the antigen recognized by SBU-1 was found to be present in the epithelial reticular cells of the fetal sheep thymus. The intensity of staining decreased as gestation progressed. In the adult thymus the antigen was mainly restricted to Hassall's corpuscles and occasional epithelial cells in the medulla. In addition, the antigen was also shown to be present in epithelial cells of the small intestine, the bronchiole, the keratinized epithelium of the rumen, and the epithelial cells of the kidney tubules. By use of immunofluorescence the antigen was shown to be present in most of the cells of wool follicles and the cortex of developing wool fibers. Western blotting of SBU-1 against the low-sulfur alpha-keratin proteins of wool confirmed that the antigen recognized by SBU-1 belongs to a family of keratins. It was concluded that SBU-1 was raised against alpha-keratin expressed by the epithelial cells of the thymic rudiment and that the expression of this antigen on the reticular network of the thymus declined with advancement of pregnancy.

Immunoperoxidase Staining of Fine-Needle Aspiration Biopsies of Renal Cell Carcinoma Using Tumor-Specific Monoclonal Antibody

1987 ◽  
Vol 13 (5) ◽  
pp. 331-333 ◽  
Author(s):  
Thomas Schärfe ◽  
Masayoshi Yokoyama ◽  
Peter Alken ◽  
Günter H. Jacobi ◽  
Rudolf Hohenfellner
Keyword(s):  
Renal Cell Carcinoma ◽  
Monoclonal Antibody ◽  
Cell Carcinoma ◽  
Fine Needle Aspiration ◽  
Renal Cell ◽  
Needle Aspiration ◽  
Immunoperoxidase Staining ◽  
Specific Monoclonal Antibody ◽  
Fine Needle

scholarly journals NO-tryptophan: a new small molecule located in the rat brain

2016 ◽  
Vol 60 (3) ◽  
Author(s):  
A. Mangas ◽  
J. Yajeya ◽  
N. González ◽  
S. Duleu ◽  
M. Geffard ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Monoclonal Antibody ◽  
Rat Brain ◽  
Morphological Characteristics ◽  
Mammalian Brain ◽  
Immunoperoxidase Technique ◽  
Specific Monoclonal Antibody ◽  
Septal Nucleus ◽  
Physiological Mechanisms ◽  
Indirect Immunoperoxidase

<p>A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10<sup>-9 </sup>M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain. The restricted distribution of NO-W in the rat brain suggests that this molecule could be involved in specific physiological mechanisms. </p>

scholarly journals Monoclonal antibody specific for carbodiimide-fixed glutamate: immunocytochemical localization in the rat CNS.

1986 ◽  
Vol 34 (3) ◽  
pp. 317-326 ◽  
Author(s):  
J E Madl ◽  
A A Larson ◽  
A J Beitz
Keyword(s):  
Monoclonal Antibody ◽  
Small Molecules ◽  
Granule Cells ◽  
Cerebellar Granule Cells ◽  
Pyramidal Cells ◽  
Keyhole Limpet Hemocyanin ◽  
Immunocytochemical Staining ◽  
Immunoperoxidase Staining ◽  
Fixed Tissue ◽  
Indirect Immunoperoxidase

Glutamate is widely distributed in the central nervous system (CNS) and is present in greater amounts than any other putative neurotransmitter. To study its distribution in the CNS, a monoclonal antibody was raised against gamma-L-glutamyl-L-glutamic acid (gamma-Glu-Glu) conjugated to keyhole limpet hemocyanin (KLH) using glutaraldehydeborohydride. By use of this antibody, indirect immunoperoxidase staining was observed in CNS tissue fixed with carbodiimide to form gamma-Glu-Glu from glutamate and post-fixed with glutaraldehyde or paraformaldehyde. In contrast, immunoreactivity was quite low in tissues fixed only with glutaraldehyde. Absorption controls indicated that the staining of carbodiimide-fixed tissue could be inhibited by micromolar concentrations of gamma-Glu-Glu but not by other small molecules. Using ELISA, the antibody reacted strongly with the gamma-Glu-Glu/KLH conjugate used to immunize the mouse, but not with other small molecules conjugated to KLH. The reactivity of the antibody with the gamma-Glu-Glu/KLH conjugate on ELISA was inhibited by free gamma-Glu-Glu in micromolar concentrations, but not by similar dipeptides or amino acids. Dense immunocytochemical staining was observed in cortical pyramidal cells, cerebellar granule cells, and the cochlear nuclei. Staining with this monoclonal antibody correlated well with other methods of localizing glutamate in the CNS.

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