Isolation and Characterization of Stem Cells from Human Central Nervous System Malignancies

2015 ◽  
pp. 33-47 ◽  
Author(s):  
Imad Saeed Khan ◽  
Moneeb Ehtesham
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Human Central Nervous System ◽  
Isolation And Characterization

scholarly journals Molecular cloning and analysis of l(1)ogre, a locus of Drosophila melanogaster with prominent effects on the postembryonic development of the central nervous system.

Genetics ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 1033-1044 ◽  
Author(s):  
T Watanabe ◽  
D R Kankel
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Postembryonic Development ◽  
P Element ◽  
Reading Frame ◽  
Isolation And Characterization ◽  
The Central Nervous System ◽  
Highly Hydrophobic ◽  
Conceptual Translation

Abstract Previous genetic studies have shown that wild-type function of the l(1)ogre (lethal (1) optic ganglion reduced) locus is essential for the generation and/or maintenance of the postembryonic neuroblasts including those from which the optic lobe is descended. In the present study molecular isolation and characterization of the l(1)ogre locus was carried out to study the structure and expression of this gene in order to gain information about the nature of l(1)ogre function and its relevance to the development of the central nervous system. About 70 kilobases (kb) of genomic DNA were isolated that spanned the region where l(1)ogre was known to reside. Southern analysis of a l(1)ogre mutation and subsequent P element-mediated DNA transformation mapped the l(1)ogre+ function within a genomic fragment of 12.5 kb. Northern analyses showed that a 2.9-kb message transcribed from this 12.5-kb region represented l(1)ogre. A 2.15-kb portion of a corresponding cDNA clone was sequenced. An open reading frame (ORF) of 1,086 base paris was found, and a protein sequence of 362 amino acids with one highly hydrophobic segment was deduced from conceptual translation of this ORF.

Transplantation of human central nervous system stem cells - neuroprotection in retinal degeneration

2012 ◽  
Vol 35 (3) ◽  
pp. 468-477 ◽  
Author(s):  
Trevor J. McGill ◽  
Benjamin Cottam ◽  
Bin Lu ◽  
Shaomei Wang ◽  
Sergej Girman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Retinal Degeneration ◽  
Human Central Nervous System

Isolation and characterization of periaxolemmal and axolemmal enriched membrane fractions from the rat central nervous system

1992 ◽  
Vol 32 (4) ◽  
pp. 593-604 ◽  
Author(s):  
V. S. Sapirstein ◽  
R. Durrie ◽  
B. Cherksey ◽  
M. E. Beard ◽  
C. J. Flynn ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Isolation And Characterization

scholarly journals Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus

2019 ◽  
Vol 23 (11) ◽  
pp. 7382-7394 ◽  
Author(s):  
Maria Teresa De Angelis ◽  
Gianluca Santamaria ◽  
Elvira Immacolata Parrotta ◽  
Stefania Scalise ◽  
Michela Lo Conte ◽  
...  
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Induced Pluripotent Stem Cells ◽  
Lupus Erythematosus ◽  
Pluripotent Stem Cells ◽  
Central Nervous System Lupus ◽  
Induced Pluripotent

scholarly journals Characterization of the Precursor Protein of the Non-Aβ Component of Senile Plaques (NACP) in the Human Central Nervous System

1996 ◽  
Vol 55 (8) ◽  
pp. 889-895 ◽  
Author(s):  
Michael C. Irizarry ◽  
Tae-Wan Kim ◽  
Megan MCNamara ◽  
Rudolph E. Tanzi ◽  
Julia M. George ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Senile Plaques ◽  
Precursor Protein ◽  
Human Central Nervous System

scholarly journals A phosphatidylinositol-linked peanut agglutinin-binding glycoprotein in central nervous system myelin and on oligodendrocytes.

1988 ◽  
Vol 106 (4) ◽  
pp. 1273-1279 ◽  
Author(s):  
D D Mikol ◽  
K Stefansson
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
White Matter ◽  
Gray Matter ◽  
Biochemical Characterization ◽  
Developmental Expression ◽  
Peanut Agglutinin ◽  
Human Central Nervous System ◽  
Binding Glycoprotein

Here we report the isolation and initial biochemical characterization of a 120-kD peanut agglutinin-binding glycoprotein from the adult human central nervous system (CNS), which is anchored to membranes through a phosphatidylinositol linkage. Myelin incubated with phosphatidylinositol-specific phospholipase C released the protein as a soluble polypeptide of 105 kD, which was isolated with peanut agglutinin-agarose affinity chromatography. The protein was found to be highly glycosylated. The protein appears to be confined to the CNS, where its developmental expression is region specific and parallels myelination. It is in greater quantity in white matter than in gray matter and it is in isolated human CNS myelin. Furthermore, ovine oligodendrocytes in culture contain the protein on their surfaces and release it into the supernatant as a soluble 105-kD form. We call this protein the oligodendrocyte-myelin protein.

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