scholarly journals A phosphatidylinositol-linked peanut agglutinin-binding glycoprotein in central nervous system myelin and on oligodendrocytes.

1988 ◽  
Vol 106 (4) ◽  
pp. 1273-1279 ◽  
Author(s):  
D D Mikol ◽  
K Stefansson
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
White Matter ◽  
Gray Matter ◽  
Biochemical Characterization ◽  
Developmental Expression ◽  
Peanut Agglutinin ◽  
Human Central Nervous System ◽  
Binding Glycoprotein

Here we report the isolation and initial biochemical characterization of a 120-kD peanut agglutinin-binding glycoprotein from the adult human central nervous system (CNS), which is anchored to membranes through a phosphatidylinositol linkage. Myelin incubated with phosphatidylinositol-specific phospholipase C released the protein as a soluble polypeptide of 105 kD, which was isolated with peanut agglutinin-agarose affinity chromatography. The protein was found to be highly glycosylated. The protein appears to be confined to the CNS, where its developmental expression is region specific and parallels myelination. It is in greater quantity in white matter than in gray matter and it is in isolated human CNS myelin. Furthermore, ovine oligodendrocytes in culture contain the protein on their surfaces and release it into the supernatant as a soluble 105-kD form. We call this protein the oligodendrocyte-myelin protein.

scholarly journals An Attenuated Variant of the GDVII Strain of Theiler’s Virus Does Not Persist and Does Not Infect the White Matter of the Central Nervous System

1999 ◽  
Vol 73 (1) ◽  
pp. 801-804 ◽  
Author(s):  
Nadine Jarousse ◽  
Ekaterina G. Viktorova ◽  
Evgeny V. Pilipenko ◽  
Vadim I. Agol ◽  
Michel Brahic
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
White Matter ◽  
Gray Matter ◽  
Noncoding Region ◽  
Theiler's Virus ◽  
Recombinant Viruses ◽  
Central Nervous Systems ◽  
The Central Nervous System

ABSTRACT The DA strain of Theiler’s virus causes a persistent and demyelinating infection of the white matter of spinal cord, whereas the GDVII strain causes a fatal gray-matter encephalomyelitis. Studies with recombinant viruses showed that this difference in phenotype is controlled mainly by the capsid. However, conflicting results regarding the existence of determinants of persistence in the capsid of the GDVII strain have been published. Here we show that a GDVII virus whose neurovirulence has been attenuated by an insertion in the 5′ noncoding region does not persist in the central nervous systems of mice. Furthermore, this virus infects the gray matter efficiently, but not the white matter. These results confirm the absence of determinants of persistence in the GDVII capsid. They suggest that the DA capsid controls persistence by allowing the virus to infect cells in the white matter of the spinal cord.

scholarly journals Dengue Virus Type 2: Protein Binding and Active Replication in Human Central Nervous System Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Ma Isabel Salazar ◽  
Marissa Pérez-García ◽  
Marisol Terreros-Tinoco ◽  
María Eugenia Castro-Mussot ◽  
Jaime Diegopérez-Ramírez ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Dengue Virus ◽  
Protein Binding ◽  
Gray Matter ◽  
Primary Cultures ◽  
Virus Protein ◽  
Specific Marker ◽  
Human Central Nervous System ◽  
Human Neurons

An increased number of dengue cases with neurological complications have been reported in recent years. The lack of reliable animal models for dengue has hindered studies on dengue virus (DENV) pathogenesis and cellular tropismin vivo. We further investigate the tropism of DENV for the human central nervous system (CNS), characterizing DENV interactions with cell surface proteins in human CNS cells by virus overlay protein binding assays (VOPBA) and coimmunoprecipitations. In VOPBA, three membrane proteins (60, 70, and 130 kDa) from the gray matter bound the entire virus particle, whereas only a 70 kDa protein bound in white matter. The coimmunoprecipitation assays revealed three proteins from gray matter consistently binding virus particles, one clearly distinguishable protein (~32 kDa) and two less apparent proteins (100 and 130 kDa). Monoclonal anti-NS3 targeted the virus protein in primary cell cultures of human CNS treated with DENV-2, which also stained positive for NeuH, a neuron-specific marker. Thus, our results indicate (1) that DENV-2 exhibited a direct tropism for human neurons and (2) that human neurons sustain an active DENV replication as was demonstrated by the presence of the NS3 viral antigen in primary cultures of these cells treated with DENV-2.

Linfoistiocitosi eritrofagocitica

2003 ◽  
Vol 16 (3) ◽  
pp. 365-372 ◽  
Author(s):  
F. Caranci ◽  
A. D'Amico ◽  
F. Briganti ◽  
R. Migliorati ◽  
C. De Fusco ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Bone Marrow ◽  
Nervous System ◽  
White Matter ◽  
White Matter Abnormalities ◽  
Diffuse White Matter ◽  
Multisystem Involvement ◽  
Identification And Characterization

Erithrophagocytic lymphohistiocytosis is a rare disease characterized by exaggerated histiocytic proliferation and activation12 and multisystem involvement including visceral organs, lymph nodes, bone marrow and central nervous system4. Magnetic Resonance (MR) examination was performed in 10 patients with previously diagnosed Erithrophagocytic lymphohistiocytosis. This study was aimed at assessing MR accuracy in the identification and characterization of central nervous system lesions. MR findings show a good correlation with areas of parenchymal and meningeal lymphohistiocytic infiltration5, demonstrating diffuse white matter abnormalities in the early stages and necrotic areas with parenchymal volume loss as terminal findings4. In addition, MR allows the follow-up after chemotherapy and bone marrow transplantation2,3. Although rare, EL should be differentiated from other pediatric patchy white matter abnormalities5.

Developmental expression and localization of MHC class I molecules in the human central nervous system

2015 ◽  
Vol 233 (9) ◽  
pp. 2733-2743 ◽  
Author(s):  
Aifeng Zhang ◽  
Hong Yu ◽  
Youji He ◽  
Yuqing Shen ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Mhc Class I ◽  
Class I ◽  
Developmental Expression ◽  
Human Central Nervous System ◽  
Mhc Class I Molecules

scholarly journals Binding of Malignant Lymphoid Cells to the White Matter of the Human Central Nervous System

1997 ◽  
Vol 56 (5) ◽  
pp. 557-568 ◽  
Author(s):  
RIITTA AHO ◽  
HANNU KALIMO ◽  
MARKO SALMI ◽  
DAVID SMITH ◽  
SIRPA JALKANEN
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
White Matter ◽  
Lymphoid Cells ◽  
Human Central Nervous System
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