Morphology Based Detection of Abnormal Red Blood Cells in Peripheral Blood Smear Images

2015 ◽  
pp. 57-60 ◽  
Author(s):  
S. Kulasekaran ◽  
Feminna Sheeba ◽  
Joy John Mammen ◽  
B. Saivigneshu ◽  
S. Mohankumar
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Peripheral Blood Smear

Convex Hull Based Detection of Overlapping Red Blood Cells in Peripheral Blood Smear Images

2015 ◽  
pp. 51-53 ◽  
Author(s):  
Feminna Sheeba ◽  
Robinson Thamburaj ◽  
Joy John Mammen ◽  
Mohan Kumar ◽  
Vansant Rangslang
Keyword(s):  
Convex Hull ◽  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Peripheral Blood Smear

Morphological Changes of Red Blood Cells in Peripheral Blood Smear of Patients with Pregnancy-related Hypertensive Disorders

2015 ◽  
Vol 46 (6) ◽  
pp. 479-483 ◽  
Author(s):  
Jorge Daniel Hernández Hernández ◽  
Onésimo Rangel Villaseñor ◽  
Javier Del Rio Alvarado ◽  
Roberto Ortega Lucach ◽  
Arturo Zárate ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Morphological Changes ◽  
Peripheral Blood Smear ◽  
Hypertensive Disorders

scholarly journals Dehydrated Hereditary Stomatocytosis: A Rare Inherited Hemolytic Anemia

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 4148-4148
Author(s):  
Farah Shaukat ◽  
Zeba Singh ◽  
Hira Latif
Keyword(s):  
Iron Overload ◽  
Red Blood Cells ◽  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Blood Transfusions ◽  
Erythroid Progenitor ◽  
Distribution Width

Abstract Background: Dehydrated hereditary stomatocytosis (DHSt), also known as hereditary xerocytosis, is a rare congenital hemolytic anemia with an autosomal dominant inheritance. It is often misdiagnosed for other hemolytic conditions, such as hereditary spherocytosis. Herein, we present the case of a young female presenting with hemolytic anemia, who was found to have a mutation in PIEZO1 gene and was subsequently diagnosed with DHSt. Case Presentation: A 39-year-old woman of Asian origin presented to the hematology clinic for evaluation of anemia diagnosed on blood work performed by primary care physician for symptoms of fatigue. She was adopted and had no information about her family history. A complete blood count revealed: hemoglobin 8.2 g/dL, mean corpuscular volume (MCV) 121.6 fL, absolute retic counts 0.08 M/mcL, lactate dehydrogenase 851 units/L and a negative coombs test. Iron profile revealed iron saturation 85% and ferritin 1961.4 ng/mL (see table 1 for laboratory work up). The peripheral blood smear showed anisopoikilocytosis, macrocytes, spherocytes and several stomatocytes along with polychromatophils. An ultrasound of the abdomen was subsequently performed, and which revealed hepatomegaly and biliary stones. Enzyme assay for glucose-6-phosphate dehydrogenase and flow cytometry for paroxysmal nocturnal hemoglobinuria were also sent and were negative. Red blood cells osmotic fragility was decreased. The bone marrow biopsy showed full spectrum trilineage hematopoiesis with no mutations on molecular testing. Based on the blood smear and clinical presentation, a diagnosis of DHSt was suspected. Genetic testing was performed and which revealed Sc.2842C>T; p.Arg948Cys mutation in the PIEZO1 gene by massively parallel sequencing and confirmation by Sanger sequencing. This confirmed the diagnosis of DHSt. Patient was started on high dose folic acid with improvement in her hemoglobin in one month. She did not require any blood transfusions. MRI liver T2* scan measured quantitative liver iron of 31 mM/g, which was at the high normal range. Discussion: DHSt is caused by gain of function mutation in PIEZO1 gene or KCCN4 gene which encode the transmembrane cation ion channel and Gardo's channel respectively on red blood cell membrane. This results in delayed inactivation of the channel. The disease presents as a spectrum from asymptomatic anemia to massive hemolysis, and many patients present later in life. Patients may manifest clinical signs of jaundice, pallor, fatigue, splenomegaly, gallstones and iron overload. Labs are typically significant for elevation in mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) and MCV, with classic slit cells red blood cells seen on peripheral blood smear (see image 1). PIEZO1 is expressed early in erythroid progenitor cells and may delay erythroid differentiation and reticulocyte maturation, which may be the cause of low reticulocyte count such as in our patient. While treatment is supportive with blood transfusions, only a minority of DHSt patients ever require regular transfusions. Interestingly, hyperferritinemia, high transferrin saturation or clinical iron overload are quite frequent in DHSt and iron chelation is recommended. Splenectomy is contraindicated due to increased risk of thrombosis. Conclusions: DHSt as a rare inherited hemolytic anemia and its diagnosis warrants maintaining a high index of clinical suspicion based on supportive laboratory findings. Diagnosis involves thorough testing earlier in the disease as patients may be asymptomatic until adulthood. Delaying the diagnosis may lead to severe iron overload and consequent organ damage. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

Analysis of Abnormality based on Size in Red Blood Cells in Peripheral Blood Smear Images

2018 ◽  
Vol 6 (9) ◽  
pp. 830-834
Author(s):  
F. Sheeba ◽  
T. Robinson ◽  
J.J. Mammen ◽  
J.M.S. Philips ◽  
T. Sathyaraj ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Peripheral Blood Smear

The Prognostic Implications of Nucleated Red Blood Cells in Myelodysplastic Syndrome

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 5058-5058
Author(s):  
Javier Munoz ◽  
Mohamad A. Younes ◽  
Marwa Hammoud ◽  
Ammar Khanshour ◽  
Rosa Michel Ortega ◽  
...  
Keyword(s):  
Myelodysplastic Syndrome ◽  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Prognostic Implication ◽  
Peripheral Smear ◽  
Nucleated Red Blood Cells ◽  
Average Survival

Abstract Abstract 5058 Background: The presence of elevated nucleated red blood cells (NRBC) in the peripheral blood smear points towards the presence of myelodysplastic syndrome (MDS) or myelophthisis. The intent of our study was to assess the prognostic implication of the presence of increased NRBC in patients with MDS. Methods: Retrospective single institution chart review over a period of 10 years from 2000 to 2010. Our study population comprised 288 diagnosed cases of MDS whose diagnoses were made by bone marrow (BM) examination. Results: 174 patients were male (60.4%) and 114 were female (39.6%). Average age at diagnosis of MDS was 71.92 years. 204 patients were Caucasian (70.8%), 69 patients were African-American (23.9%), 1 patient was Hispanic, 2 patients were Asian, and race was not documented in 12 patients. 117 patients had intermediate-1 IPSS (40.6%), 79 had low IPSS (27.4%), 54 patients had intermediate-2 IPSS (18.7%), 26 patients had high IPSS (9.0%), and 12 patients had unknown IPSS. 131 patients (45.5%) had undetectable NRBC in their peripheral smear prior to their initial diagnostic BM examination. 13 out of these 131 patients (9.92%) progressed to acute myeloid leukemia (AML). 157 patients (54.5%) had one or more NRBC in their peripheral smear prior to their diagnostic BM examination. 27 out of these 157 patients (17.19%) progressed to AML. Survival in days was available in 59 patients. 19 out of 59 patients had undetectable NRBC in their peripheral smear prior to their initial diagnostic BM examination with an average survival of 1,256.2 days. Only one patient in this group underwent allogeneic matched unrelated peripheral stem cell transplantation (PSCT) with a survival of 2,532 days. 40 out of 59 patients had one or more NRBC in their peripheral smear prior to their diagnostic BM examination with an average survival of 850.4 days. Only 3 patients in this group underwent allogeneic matched PSCT (2 related and 1 unrelated) with an average survival of 924.3 days. Conclusions: The presence of nucleated red blood cells in the peripheral blood smear of patients with MDS seems to be a marker of more aggressive disease, higher rates of progression to AML and portends worse survival. To our knowledge this is the first study documenting the prognostic implication of NRBC in the peripheral circulation of patients with MDS. Disclosures: No relevant conflicts of interest to declare.

scholarly journals OVERVIEW OF MALARIA PARASITE AND ITS PREVENTION IN INDIA

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Adil Raza ◽  
Megha Chaudhary ◽  
Sonika Devi
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Plasmodium Species ◽  
Peripheral Blood Smear ◽  
Infected Mosquito ◽  
Protozoan Parasites ◽  
Malaria Parasites ◽  
Genus Plasmodium

Background: Malaria is a systematic disease caused by a parasite called Plasmodium which is transmitted into the human blood via female Anopheles mosquito. Malaria in humans is caused by four species of protozoan parasites of the genus Plasmodium: P. falciparum, P. vivax, P. ovale, and P. malariae. The parasite enters the human body through a mosquito bite and travel to the very crucial organ, the liver, where they multiply and come back to the bloodstream and destroy red blood cells. Malaria causes symptoms that typically include fever, tiredness, vomiting, and headaches. In severe cases it can cause yellow skin, seizures, coma, or death. Symptoms usually begin ten to fifteen days after being bitten by an infected mosquito. In those who have recently survived an infection, reinfection usually causes milder symptoms. Objectives: Isolation of different species of malaria parasites. The prevalence of malaria parasite in India. Methods: The procedure follows these steps: collection of peripheral blood, staining of smear with Leishman’s stain and examination of red blood cells for malaria parasites under the microscope. Results: We observed the plasmodium species in peripheral blood smear. Conclusion: Worldwide, the number of cases of malaria caused by Plasmodium falciparum, the most dangerous species of the parasite, is on the rise.

Development of a robust algorithm for detection of nuclei of white blood cells in peripheral blood smear images

2019 ◽  
Vol 78 (13) ◽  
pp. 17879-17898 ◽  
Author(s):  
Roopa B. Hegde ◽  
Keerthana Prasad ◽  
Harishchandra Hebbar ◽  
Brij Mohan Kumar Singh
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Robust Algorithm
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