Membrane Protein Production in E. coli for Applications in Drug Discovery

2016 ◽  
pp. 59-77 ◽  
Author(s):  
Harm Jan Snijder ◽  
Jonna Hakulinen
Keyword(s):  
Drug Discovery ◽  
Membrane Protein ◽  
Protein Production ◽  
E Coli

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs

2017 ◽  
pp. 291-312 ◽  
Author(s):  
Ralf-Bernhardt Rues ◽  
Alexander Gräwe ◽  
Erik Henrich ◽  
Frank Bernhard
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
E Coli

scholarly journals Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Thomas Baumgarten ◽  
Susan Schlegel ◽  
Samuel Wagner ◽  
Mirjam Löw ◽  
Jonas Eriksson ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
E Coli ◽  
Isolation And Characterization ◽  
Production Strain

Optimization of Membrane Protein Production Using Titratable Strains of E. coli

2017 ◽  
pp. 83-107
Author(s):  
Rosa Morra ◽  
Kate Young ◽  
David Casas-Mao ◽  
Neil Dixon ◽  
Louise E. Bird
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
E Coli

Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions

2017 ◽  
pp. 109-126 ◽  
Author(s):  
Grietje Kuipers ◽  
Markus Peschke ◽  
Nurzian Bernsel Ismail ◽  
Anna Hjelm ◽  
Susan Schlegel ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
E Coli

Membrane protein production and formulation for drug discovery

2021 ◽  
Vol 42 (8) ◽  
pp. 657-674
Author(s):  
Ellen Gulezian ◽  
Christina Crivello ◽  
Janna Bednenko ◽  
Claudia Zafra ◽  
Yihui Zhang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Membrane Protein ◽  
Protein Production

scholarly journals Simple Genetic Selection Protocol for Isolation of Overexpressed Genes That Enhance Accumulation of Membrane-Integrated Human G Protein-Coupled Receptors in Escherichia coli

2010 ◽  
Vol 76 (17) ◽  
pp. 5852-5859 ◽  
Author(s):  
Georgios Skretas ◽  
George Georgiou
Keyword(s):  
Escherichia Coli ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
G Protein ◽  
Protein Production ◽  
Genetic Selection ◽  
G Protein Coupled Receptors ◽  
Efficient Production ◽  
E Coli ◽  
G Protein Coupled

ABSTRACT The efficient production of membrane proteins in bacteria remains a major challenge. In this work, we sought to identify overexpressed genes that enhance the yields of recombinant membrane proteins in Escherichia coli. We developed a genetic selection system for bacterial membrane protein production, consisting of membrane protein fusions with the enzyme β-lactamase and facile selection of high-production strains on ampicillin-containing media. This system was used to screen the ASKA library, an ordered library of plasmids encoding all the known E. coli open reading frames (ORFs), and several clones with the ability to accumulate enhanced amounts of recombinant membrane proteins were selected. Notably, coexpression of ybaB, a gene encoding a putative DNA-binding protein of unknown function, was found to enhance the accumulation of a variety of membrane-integrated human G protein-coupled receptors and other integral membrane proteins in E. coli by up to 10-fold. The results of this study highlight the power of genetic approaches for identifying factors that impact membrane protein biogenesis and for generating engineered microbial hosts for membrane protein production.

Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions

2013 ◽  
pp. 381-400 ◽  
Author(s):  
Anna Hjelm ◽  
Susan Schlegel ◽  
Thomas Baumgarten ◽  
Mirjam Klepsch ◽  
David Wickström ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
E Coli
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