Clinical Islet Isolation

2016 ◽  
pp. 89-122 ◽  
Author(s):  
Wayne J. Hawthorne ◽  
Lindy Williams ◽  
Yi Vee Chew
Keyword(s):  
Islet Isolation

Different roles of class I and class II Clostridium histolyticum collagenase in rat pancreatic islet isolation

Diabetes ◽  
1995 ◽  
Vol 44 (2) ◽  
pp. 227-233 ◽  
Author(s):  
G. H. Wolters ◽  
G. H. Vos-Scheperkeuter ◽  
H. C. Lin ◽  
R. van Schilfgaarde
Keyword(s):  
Pancreatic Islet ◽  
Class Ii ◽  
Class I ◽  
Islet Isolation ◽  
Clostridium Histolyticum ◽  
Pancreatic Islet Isolation ◽  
Clostridium Histolyticum Collagenase ◽  
Rat Pancreatic Islet

scholarly journals Hypothermic oxygenated Machine Perfusion of the Human Pancreas for Clinical Islet Isolation: A Prospective Feasibility Study

2021 ◽  
Author(s):  
Jason B. Doppenberg ◽  
Marjolein Leemkuil ◽  
Marten A. Engelse ◽  
Christina Krikke ◽  
Eelco J.P. de Koning ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Machine Perfusion

scholarly journals Evaluation of Viable β-Cell Mass is Useful for Selecting Collagenase for Human Islet Isolation: Comparison of Collagenase NB1 and Liberase HI

2012 ◽  
Vol 21 (1) ◽  
pp. 39-47 ◽  
Author(s):  
R. Misawa ◽  
C. Ricordi ◽  
A. Miki ◽  
S. Barker ◽  
R. D. Molano ◽  
...  
Keyword(s):  
Cell Mass ◽  
Human Islet ◽  
Islet Isolation ◽  
Β Cell

scholarly journals Application of Digital Image Analysis to Determine Pancreatic Islet Mass and Purity in Clinical Islet Isolation and Transplantation

2015 ◽  
Vol 24 (7) ◽  
pp. 1195-1204 ◽  
Author(s):  
Ling-Jia Wang ◽  
Hermann J. Kissler ◽  
Xiaojun Wang ◽  
Olivia Cochet ◽  
Adam Krzystyniak ◽  
...  
Keyword(s):  
Image Analysis ◽  
Pancreatic Islet ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Islet Isolation ◽  
Islet Mass

scholarly journals Implication of Mitochondrial Cytoprotection in Human Islet Isolation and Transplantation

2012 ◽  
Vol 2012 ◽  
pp. 1-16 ◽  
Author(s):  
Yong Wang ◽  
Joshua E. Mendoza-Elias ◽  
Meirigeng Qi ◽  
Tricia A. Harvat ◽  
Sang Joon Ahn ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Islet Transplantation ◽  
Mitochondrial Function ◽  
Graft Function ◽  
Complex Nature ◽  
Islet Isolation ◽  
Success Rates ◽  
Mitochondrial Integrity ◽  
Multistep Process ◽  
Underlying Mechanisms

Islet transplantation is a promising therapy for type 1 diabetes mellitus; however, success rates in achieving both short- and long-term insulin independence are not consistent, due in part to inconsistent islet quality and quantity caused by the complex nature and multistep process of islet isolation and transplantation. Since the introduction of the Edmonton Protocol in 2000, more attention has been placed on preserving mitochondrial function as increasing evidences suggest that impaired mitochondrial integrity can adversely affect clinical outcomes. Some recent studies have demonstrated that it is possible to achieve islet cytoprotection by maintaining mitochondrial function and subsequently to improve islet transplantation outcomes. However, the benefits of mitoprotection in many cases are controversial and the underlying mechanisms are unclear. This article summarizes the recent progress associated with mitochondrial cytoprotection in each step of the islet isolation and transplantation process, as well as islet potency and viability assays based on the measurement of mitochondrial integrity. In addition, we briefly discuss immunosuppression side effects on islet graft function and how transplant site selection affects islet engraftment and clinical outcomes.

scholarly journals Islet isolation assessment in man and large animals

1990 ◽  
Vol 27 (3) ◽  
pp. 185-195 ◽  
Author(s):  
Camillo Ricordi ◽  
Derek W. R. Gray ◽  
Bernhard J. Hering ◽  
Dixon B. Kaufman ◽  
Garth L. Warnock ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Large Animals

Modified Two-Layer Preservation Method (M-Kyoto/PFC) Improves Islet Yields in Islet Isolation

2006 ◽  
Vol 6 (3) ◽  
pp. 496-504 ◽  
Author(s):  
H. Noguchi ◽  
M. Ueda ◽  
Y. Nakai ◽  
Y. Iwanaga ◽  
T. Okitsu ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Preservation Method

Intraoperative islet isolation for pancreas autotransplantation: A novel technique

Pancreatology ◽  
2013 ◽  
Vol 13 (2) ◽  
pp. e21-e22
Author(s):  
M. Cooper ◽  
N. Desai ◽  
K. Hirose ◽  
Z. Sun ◽  
D. Warren ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Novel Technique

Prevention of oxidative stress in porcine islet isolation

2010 ◽  
Vol 13 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Philipp Stiegler ◽  
Vanessa Stadlbauer ◽  
Florian Hackl ◽  
Silvia Schaffellner ◽  
Florian Iberer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Islet Isolation ◽  
Porcine Islet
Sign in / Sign up

Export Citation Format

Share Document