Amplified Fragment Length Polymerase-Based Phylogenetic Relationships of Heirloom Tomato and Dark Green Vegetable Accessions

2019 ◽  
pp. 47-59
Author(s):  
Ahmad Naseer Aziz
Keyword(s):  
Fragment Length ◽  
Phylogenetic Relationships ◽  
Green Vegetable ◽  
Dark Green

Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 216-223 ◽  
Author(s):  
Ram G. Nadimpalli ◽  
R. L. Jarret ◽  
Sharad C. Phatak ◽  
Gary Kochert
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Phylogenetic Relationships ◽  
Seed Protein ◽  
Restriction Enzymes ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Genomic Probes ◽  
Clustering Patterns ◽  
Restriction Fragment Length

Nuclear restriction fragment length polymorphisms (RFLPs) were used to determine phylogenetic relationships in the genus Cajanus using 15 random genomic probes and six restriction enzymes. Twenty-four accessions representing 12 species of four genera (Cajanus, Dunbaria, Eriosema, and Rhynchosia) were examined to determine phylogenetic relationships in the genus Cajanus. Eriosema parviflorum was selected as the out-group. Sufficient RFLP polymorphisms were detected among species to resolve in-group taxa into distinct clusters. Topologies of trees from parsimony and similarity matrix analyses were similar but not identical, and clustering patterns agreed broadly with published phylogenies based on seed protein data and, to a lesser extent, data from cytology and breeding experiments. Accessions of cultivated C. cajan shared more DNA fragments with C. scarabaeoides than with C. cajanifolia. Inconsistencies in taxonomic relationships based on data from morphology, cytology, crossability, and RFLPs are discussed.Key words: pigeonpea, systematics, taxonomy, evolution, germplasm.

scholarly journals 336 PHYLOGENETIC RELATIONSHIPS OF CELERY AND ITS WILD RELATIVES BASED ON RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 478f-478
Author(s):  
Gordon M. Huestis ◽  
Carlos F. Quiros
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Chloroplast Dna ◽  
Southern Hemisphere ◽  
Fragment Length ◽  
Wild Species ◽  
Phylogenetic Relationships ◽  
Wild Relatives ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Phylogenetic relationships of seven Apium species, including three horticultural types of A. graveolens, were assayed for RFLPs using cDNA, chloroplast DNA, and rDNA probes. Most of the probes had been previously mapped in celery. The three horticultural types of A. graveolens were found to be less polymorphic than the wild species and in phylogenetic analysis they clustered together. The wild species formed a cluster on the dendrogram corresponding to their origin in the southern hemisphere. A. nodiflorum, a wild species from Ethiopia formed a branch on the phylogenetic tree apart from all other species. This, along with morphological considerations, suggests that A. nodiflorum should be reclassified outside the genus Apium.

Development of DNA fingerprinting keys for discrimination of Cicer echinospermum (P.H. Davis) accessions using AFLP markers

2004 ◽  
Vol 55 (9) ◽  
pp. 947 ◽  
Author(s):  
Fucheng Shan ◽  
Heather Clarke ◽  
Guijun Yan ◽  
Julie A. Plummer ◽  
Kadambot H. M. Siddique
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Dna Markers ◽  
Phylogenetic Relationships ◽  
Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Close Relative ◽  
Reliable Technique ◽  
Cicer Echinospermum ◽  
Improvement Programs

To test the hypothesis that DNA markers associated with specific genetic make-up can be detected and used to discriminate genotypes, amplified fragment length polymorphism (AFLP) markers were produced for 14 accessions in Cicer echinospermum, a close relative of Cicer arietinum (chickpea). Six selective amplification primer combinations produced high polymorphism with average polymorphic loci of 77.2%. The polymorphism detected in this study enabled fingerprinting keys to be established to discriminate accessions within C. echinospermum. Results showed that molecular analysis using AFLP was a good and reliable technique to differentiate C. echinospermum accessions and to reconstruct phylogenetic relationships between them, which could help parental selection in chickpea improvement programs.

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