Phylogenetic relationships of the pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 216-223 ◽  
Author(s):  
Ram G. Nadimpalli ◽  
R. L. Jarret ◽  
Sharad C. Phatak ◽  
Gary Kochert
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Phylogenetic Relationships ◽  
Seed Protein ◽  
Restriction Enzymes ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Genomic Probes ◽  
Clustering Patterns ◽  
Restriction Fragment Length

Nuclear restriction fragment length polymorphisms (RFLPs) were used to determine phylogenetic relationships in the genus Cajanus using 15 random genomic probes and six restriction enzymes. Twenty-four accessions representing 12 species of four genera (Cajanus, Dunbaria, Eriosema, and Rhynchosia) were examined to determine phylogenetic relationships in the genus Cajanus. Eriosema parviflorum was selected as the out-group. Sufficient RFLP polymorphisms were detected among species to resolve in-group taxa into distinct clusters. Topologies of trees from parsimony and similarity matrix analyses were similar but not identical, and clustering patterns agreed broadly with published phylogenies based on seed protein data and, to a lesser extent, data from cytology and breeding experiments. Accessions of cultivated C. cajan shared more DNA fragments with C. scarabaeoides than with C. cajanifolia. Inconsistencies in taxonomic relationships based on data from morphology, cytology, crossability, and RFLPs are discussed.Key words: pigeonpea, systematics, taxonomy, evolution, germplasm.

Molecular cloning of a bovine ornithine decarboxylase cDNA and its use in the detection of restriction fragment length polymorphisms in Holsteins

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 325-331 ◽  
Author(s):  
Jianbo Yao ◽  
David Zadworny ◽  
Urs Kühnlein ◽  
J. Flan Hayes
Keyword(s):  
Ornithine Decarboxylase ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Molecular Size ◽  
Restriction Enzymes ◽  
Bovine Liver ◽  
Single Species ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

A cDNA coding for ornithine decarboxylase (ODC) was isolated from a bovine liver cDNA library. The clone (1758 base pairs) consisted of 5′- and 3′-untranslated regions of 185 and 187 nucleotides, respectively, and an open reading frame of 1383 nucleotides encoding an ODC protein (Mr 51 342 daltons) of 461 amino acids. Comparison of the nucleotide and the predicted amino acid of the cDNA with other mammalian ODCs showed a very high degree of homology both at the DNA and protein levels. The bovine ODC mRNA was identified by northern blot to be a single species with a molecular size of 2.35 kilobase pairs. Primer extension analysis indicated that the 5′-untranslated region of the bovine ODC mRNA was 312 nucleotides long. Southern blot analysis of bovine genomic DNA revealed restriction fragment length polymorphisms when cleaved with restriction enzymes PstI, MspI, TaqI, and BglI.Key words: bovine, ornithine decarboxylase, cloning, restriction fragment length polymorphism.

Restriction Fragment Length Polymorphisms in Chloroplast DNA From Six Species of Eucalyptus

1991 ◽  
Vol 39 (5) ◽  
pp. 399 ◽  
Author(s):  
DA Steane ◽  
AK West ◽  
BM Potts ◽  
JR Ovenden ◽  
JB Reid
Keyword(s):  
Chloroplast Dna ◽  
Chloroplast Genome ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Enzymes ◽  
Restriction Fragment Length Polymorphisms ◽  
Taxonomic Distance ◽  
Length Polymorphisms ◽  
Well Differentiated ◽  
Restriction Fragment Length

Chloroplast DNA was extracted from six species of Eucalyptus (E. perriniana, E. nitens, E. ovata, E. regnans, E. amygdalina and E. risdonii). Digests with four restriction enzymes (Hind III, Xho I, Nco I and Eco RV) revealed restriction fragment length polymorphisms (RFLPs) between subgenera, between species and within species. However, no variation in fragment pattern was detected with Sac II or Pst I. The subgenera Monocalyptus and Symphyomyrtus were clearly differentiated by their RFLP patterns where, with the exception of one outlying specimen of E. amygdalina, 45% of all polymorphic fragments were specific to one or other subgenus. While species from different subgenera and series were well differentiated, it was more difficult to differentiate species within series with the low sample sizes used. However, the average net divergence between species increased with increasing taxonomic distance between species, from 0.02% within series and 0.20% between species from different series within subgenera, to 0.99% of nucleotides per nucleotide site for species from different subgenera. Based on Eco RV digests, the eucalypt chloroplast genome was estimated at 143 kb.

scholarly journals A survey of restriction fragment length polymorphisms in tall fescue and its relatives

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 686-692 ◽  
Author(s):  
W. W. Xu ◽  
David A. Sleper ◽  
David A. Hoisington
Keyword(s):  
Restriction Fragment ◽  
Tall Fescue ◽  
Fragment Length ◽  
Festuca Arundinacea ◽  
Genomic Library ◽  
Restriction Enzymes ◽  
Single Copy ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms (RFLPs) have several advantages over conventional genetic markers and as a result have received increased attention from plant breeders and geneticists. The objective of this study was to construct a tall fescue (Festuca arundinacea Schreb.) genomic library and to survey RFLPs in tall fescue and its relatives. Using plasmid pUC19 as a vector and Escherichia coli XL1-Blue cells as hosts, the first reported PstI genomic DNA library has been established from hexaploid (2n = 6x = 42) tall fescue. The genomic clones were evaluated using nine genotypes from three species of Festuca and three restriction enzymes (BamHI, EcoRI, and HindIII). One hundred and seventy-four probes gave readable results, of which 21% were repetitive and 79% single-copy. The single-copy probes revealed good polymorphism in tall fescue. Approximately 21% of the probes did not cross hybridize to any of the diploids or tetraploids or both and, therefore, represented genome-specific clones.Key words: tall fescue, genome-specific probes, genomic library, polyploids, genomic relationships

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

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