Rapid and Sensitive Detection of Heterozygous Deletions of one or more Exons in Hemophilia A Females by Multiplex PCR and DHPLC Technique

2007 ◽  
pp. 213-216
Author(s):  
A. Pavlova ◽  
J. Schröder ◽  
D. Delev ◽  
C. R. Müller ◽  
E. Seifried ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Hemophilia A ◽  
Sensitive Detection

scholarly journals 16S rRNA Sequence-based Rapid and Sensitive Detection of Aquatic Bacteria by On-chip Hybridization Following Multiplex PCR

2008 ◽  
Vol 54 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Tomoaki Ichijo ◽  
Nobuyasu Yamaguchi ◽  
Katsuji Tani ◽  
Masao Nasu
Keyword(s):  
16S Rrna ◽  
Multiplex Pcr ◽  
Sensitive Detection ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Aquatic Bacteria ◽  
On Chip

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

2012 ◽  
Vol 65 (1) ◽  
pp. 44-53 ◽  
Author(s):  
B. Padmavathy ◽  
R. Vinoth Kumar ◽  
Amee Patel ◽  
S. Deepika Swarnam ◽  
T. Vaidehi ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Sensitive Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Electrical microarrays for highly sensitive detection of multiplex PCR products from biological agents

2009 ◽  
Vol 24 (6) ◽  
pp. 1737-1743 ◽  
Author(s):  
Bruno Elsholz ◽  
Andreas Nitsche ◽  
John Achenbach ◽  
Heinz Ellerbrok ◽  
Lars Blohm ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Biological Agents ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Pcr Products ◽  
Highly Sensitive Detection

scholarly journals Polar Body Diagnosis for Hemophilia A Using Multiplex PCR for Linked Polymorphic Markers

2005 ◽  
Vol 53 (3) ◽  
pp. 277-280 ◽  
Author(s):  
Diana Tomi ◽  
Georg Griesinger ◽  
Askan Schultze-Mosgau ◽  
Juliane Eckhold ◽  
Beate Schöpper ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Preimplantation Genetic Diagnosis ◽  
Hemophilia A ◽  
Polar Body ◽  
Genetic Diagnosis ◽  
Polymorphic Markers ◽  
Polar Bodies ◽  
Fluorescent Pcr ◽  
Polar Body Diagnosis

Preimplantation genetic diagnosis (PGD) is usually performed on blastomeres. In Germany, the only possibility to perform PGD is by analysis of polar bodies. We performed PGD using polar bodies in a woman who is a carrier of hemophilia A. Multiplex PCR followed by nested fluorescent PCR for five linked polymorphic markers was established. From 11 analyzed polar bodies, only 1 showed alleles linked to the mutation. The corresponding oocyte was transferred and no pregnancy was established. As seen in other investigations, the rate of heterozygous first polar bodies is surprisingly high.

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