Cholesterol Dependence of Cell Membrane Dynamics Proved by Fluorescence Spectroscopy and Imaging

2007 ◽  
pp. 372-377 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Wolfgang S. L. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Fluorescence Spectroscopy ◽  
Membrane Dynamics

scholarly journals Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies

2007 ◽  
Vol 179 (5) ◽  
pp. 1067-1082 ◽  
Author(s):  
Valeria R. Caiolfa ◽  
Moreno Zamai ◽  
Gabriele Malengo ◽  
Annapaola Andolfo ◽  
Chris D. Madsen ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Plasminogen Activator ◽  
Fluorescent Protein ◽  
Surface Protein ◽  
Basal Membrane ◽  
Membrane Dynamics ◽  
Live Cells ◽  
Cell Surface Protein ◽  
Protein Assemblies

To search for functional links between glycosylphosphatidylinositol (GPI) protein monomer–oligomer exchange and membrane dynamics and confinement, we studied urokinase plasminogen activator (uPA) receptor (uPAR), a GPI receptor involved in the regulation of cell adhesion, migration, and proliferation. Using a functionally active fluorescent protein–uPAR in live cells, we analyzed the effect that extracellular matrix proteins and uPAR ligands have on uPAR dynamics and dimerization at the cell membrane. Vitronectin directs the recruitment of dimers and slows down the diffusion of the receptors at the basal membrane. The commitment to uPA–plasminogen activator inhibitor type 1–mediated endocytosis and recycling modifies uPAR diffusion and induces an exchange between uPAR monomers and dimers. This exchange is fully reversible. The data demonstrate that cell surface protein assemblies are important in regulating the dynamics and localization of uPAR at the cell membrane and the exchange of monomers and dimers. These results also provide a strong rationale for dynamic studies of GPI-anchored molecules in live cells at steady state and in the absence of cross-linker/clustering agents.

Membrane Dynamics: Fluorescence Spectroscopy

2000 ◽  
pp. 1-29
Author(s):  
Yamunadevi Subburaj ◽  
Raquel Salvador Gallego ◽  
Ana J. García-Sáez
Keyword(s):  
Fluorescence Spectroscopy ◽  
Membrane Dynamics

scholarly journals Application of Spot Variation FCS (svFCS) Analysis to T Cell Membrane Dynamics

2020 ◽  
Vol 118 (3) ◽  
pp. 353a
Author(s):  
Yannick Hamon ◽  
Anne-Marie Sartre ◽  
Anthony Formisano ◽  
Sébastien Mailfert ◽  
Didier Marguet ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Membrane ◽  
Membrane Dynamics ◽  
T Cell Membrane

Cholesterol dependence of cell membrane dynamics

2005 ◽  
Author(s):  
Petra Weber ◽  
Michael Wagner ◽  
Reinhard Sailer ◽  
Wolfgang S. Strauss ◽  
Herbert Schneckenburger
Keyword(s):  
Cell Membrane ◽  
Membrane Dynamics

scholarly journals 2P213 Scanning ion conductance microscopy for measuring single cell membrane fluctuations(13B. Biological & Artifical membrane: Dynamics,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S194
Author(s):  
Zen Ishikura ◽  
Yusuke Mizutani ◽  
Kaori Kuribayashi-Shigetomi ◽  
Yuuki Fujii ◽  
Myung-Hoon Choi ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Membrane Dynamics ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations

scholarly journals Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces

BMC Biology ◽  
2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Marion Bichet ◽  
Bastien Touquet ◽  
Virginie Gonzalez ◽  
Isabelle Florent ◽  
Markus Meissner ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Host Cell ◽  
Membrane Dynamics ◽  
Host Cell Membrane ◽  
Myosin Motors

Simultaneous manipulation and detection of living cell membrane dynamics

2007 ◽  
Vol 32 (13) ◽  
pp. 1893 ◽  
Author(s):  
Michael Gögler ◽  
Timo Betz ◽  
Josef Alfons Käs
Keyword(s):  
Cell Membrane ◽  
Living Cell ◽  
Membrane Dynamics

scholarly journals 1P220 Dynamic Response of Menthol on Thermo-Induced Cell Membrane: More than Receptors(13B. Biological & Artifical membrane: Dynamics,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S142
Author(s):  
Pooja Gusain ◽  
Neha Sharma ◽  
Tsuyoshi Yoda ◽  
Masahiro Takagi
Keyword(s):  
Cell Membrane ◽  
Dynamic Response ◽  
Membrane Dynamics
Sign in / Sign up

Export Citation Format

Share Document