Signaling in Vesicle Traffic: Protein-Lipid Interface in Regulation of Plant Endomembrane Dynamics

2009 ◽  
pp. 107-133 ◽  
Author(s):  
V. Žárský ◽  
M. Potocký
Keyword(s):  
Vesicle Traffic

scholarly journals Expression of familial Alzheimer disease presenilin 1 gene attenuates vesicle traffic and reduces peptide secretion in cultured astrocytes devoid of pathologic tissue environment

Glia ◽  
2015 ◽  
Vol 64 (2) ◽  
pp. 317-329 ◽  
Author(s):  
Matjaž Stenovec ◽  
Saša Trkov ◽  
Eva Lasič ◽  
Slavica Terzieva ◽  
Marko Kreft ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Presenilin 1 ◽  
Vesicle Traffic ◽  
Cultured Astrocytes ◽  
Familial Alzheimer Disease ◽  
Peptide Secretion

scholarly journals Pmt-Mediated O Mannosylation Stabilizes an Essential Component of the Secretory Apparatus, Sec20p, in Candida albicans

2004 ◽  
Vol 3 (5) ◽  
pp. 1164-1168 ◽  
Author(s):  
Yvonne Weber ◽  
Stephan K.-H. Prill ◽  
Joachim F. Ernst
Keyword(s):  
Endoplasmic Reticulum ◽  
Candida Albicans ◽  
Fungal Pathogen ◽  
Wild Type ◽  
Rapid Degradation ◽  
Vesicle Traffic ◽  
Human Fungal Pathogen ◽  
Low Levels ◽  
Lumenal Domain ◽  
Secretory Apparatus

ABSTRACT Sec20p is an essential endoplasmic reticulum (ER) membrane protein in yeasts, functioning as a tSNARE component in retrograde vesicle traffic. We show that Sec20p in the human fungal pathogen Candida albicans is extensively O mannosylated by protein mannosyltransferases (Pmt proteins). Surprisingly, Sec20p occurs at wild-type levels in a pmt6 mutant but at very low levels in pmt1 and pmt4 mutants and also after replacement of specific Ser/Thr residues in the lumenal domain of Sec20p. Pulse-chase experiments revealed rapid degradation of unmodified Sec20p (38.6 kDa) following its biosynthesis, while the stable O-glycosylated form (50 kDa) was not formed in a pmt1 mutant. These results suggest a novel function of O mannosylation in eukaryotes, in that modification by specific Pmt proteins will prevent degradation of ER-resident membrane proteins via ER-associated degradation or a proteasome-independent pathway.

scholarly journals Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis

2009 ◽  
Vol 50 (12) ◽  
pp. 2023-2033 ◽  
Author(s):  
Kohei Hamaji ◽  
Megumi Nagira ◽  
Katsuhisa Yoshida ◽  
Miwa Ohnishi ◽  
Yoshihisa Oda ◽  
...  
Keyword(s):  
Salt Stress ◽  
Ion Accumulation ◽  
Vesicle Traffic

scholarly journals Divergence of Eukaryotic Secretory Components: the Candida albicans Homolog of the Saccharomyces cerevisiae Sec20 Protein Is N Terminally Truncated, and Its Levels Determine Antifungal Drug Resistance and Growth

2001 ◽  
Vol 183 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Yvonne Weber ◽  
Uwe J. Santore ◽  
Joachim F. Ernst ◽  
Rolf K. Swoboda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Transcriptional Control ◽  
Secretory Pathway ◽  
Sodium Dodecyl ◽  
Fungal Species ◽  
Gene Encoding ◽  
Vesicle Traffic ◽  
Antifungal Drug Resistance ◽  
And Function

ABSTRACT Sec20p is a component of the yeast Saccharomyces cerevisiae secretory pathway that does not have a close homolog in higher eukaryotic cells. To verify the function of Sec20p in other fungal species, we characterized the gene encoding a Sec20p homolog in the human fungal pathogen Candida albicans. The deduced protein has 27% identity with, but is missing about 100 N-terminal residues compared to S. cerevisiae Sec20p, which is part of the cytoplasmic tail interacting with the cytoplasmic protein Tip20p. Because a strain lacking both C. albicans SEC20alleles could not be constructed, we placed SEC20 under transcriptional control of two regulatable promoters, MET3pand PCK1p. Repression of SEC20 expression in these strains prevented (MET3p-SEC20 allele) or retarded (PCK1p-SEC20 allele) growth and led to the appearance of extensive intracellular membranes, which frequently formed stacks. Reduced SEC20 expression in the PCK1p-SEC20strain did not affect morphogenesis but led to a series of hypersensitivity phenotypes including supersensitivity to aminoglycoside antibiotics, to nystatin, to sodium dodecyl sulfate, and to cell wall inhibitors. These results demonstrate the occurrence and function of Sec20p in a fungal species other than S. cerevisiae, but the lack of the N-terminal domain and the apparent absence of a close TIP20 homolog in the C. albicans genome also indicate a considerable diversity in mechanisms of retrograde vesicle traffic in eukaryotes.

Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic

2000 ◽  
Vol 114 (3) ◽  
pp. 219-228 ◽  
Author(s):  
Nadia A. Ameen ◽  
Elly van Donselaar ◽  
George Posthuma ◽  
Hugo de Jonge ◽  
Gwenn McLaughlin ◽  
...  
Keyword(s):  
Subcellular Distribution ◽  
Rat Intestine ◽  
Vesicle Traffic ◽  
Physiologic Role

Synaptic Vesicle Traffic

2001 ◽  
Author(s):  
David Dunlap ◽  
Flavia Valtorta ◽  
Riccardo Fesce
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Traffic

A formal methods approach to predicting new features of the eukaryotic vesicle traffic system

2019 ◽  
Author(s):  
Arnab Bhattacharyya ◽  
Ashutosh Gupta ◽  
Lakshmanan Kuppusamy ◽  
Somya Mani ◽  
Ankit Shukla ◽  
...  
Keyword(s):  
Formal Methods ◽  
Vesicle Traffic ◽  
Traffic System

scholarly journals Modeling Vesicle Traffic Reveals Unexpected Consequences for Cdc42p-Mediated Polarity Establishment

2011 ◽  
Vol 21 (3) ◽  
pp. 184-194 ◽  
Author(s):  
Anita T. Layton ◽  
Natasha S. Savage ◽  
Audrey S. Howell ◽  
Susheela Y. Carroll ◽  
David G. Drubin ◽  
...  
Keyword(s):  
Vesicle Traffic ◽  
Polarity Establishment ◽  
Unexpected Consequences
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