Standard Methods for Standard Options

2009 ◽  
pp. 1-61
Author(s):  
Rüdiger U. Seydel
Keyword(s):  
Standard Methods

3-D reconstruction of molecular shape from microcrystal thin sections

1983 ◽  
Vol 41 ◽  
pp. 748-749
Author(s):  
S. Cusack ◽  
J.-C. Jésior
Keyword(s):  
Three Dimensional ◽  
Molecular Shape ◽  
Biological Molecules ◽  
Fourier Space ◽  
Single Particles ◽  
Thin Sections ◽  
Standard Methods ◽  
X Ray ◽  
X Ray Crystallography ◽  
Dimensional Reconstruction

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).

Ultrastructural Localization of Phycocyanin in the Acidophilic, Thermophilic Alga, Cyanidium Caldarium

1973 ◽  
Vol 31 ◽  
pp. 462-463
Author(s):  
M. R. Edwards ◽  
J. D. Mainwaring
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Ultrastructural Localization ◽  
Review Of Literature ◽  
Cyanidium Caldarium ◽  
Taxonomic Rank ◽  
Thin Sections ◽  
Standard Methods ◽  
Laboratory Cultures

Although the general ultrastructure of Cyanidium caldarium, an acidophilic, thermophilic alga of questionable taxonomic rank, has been extensively studied (see review of literature in reference 1), some peculiar ultrastructural features of the chloroplast of this alga have not been noted by other investigators.Cells were collected and prepared for thin sections at the Yellowstone National Park and were also grown in laboratory cultures (45-52°C; pH 2-5). Fixation (glutaraldehyde-osmium), dehydration (ethanol), and embedding (Epon 812) were accomplished by standard methods. Replicas of frozenfracture d- etched cells were obtained in a Balzers apparatus. In addition, cells were examined after disruption in a French Press.

A Device for Magnification Measurement

1972 ◽  
Vol 30 ◽  
pp. 622-623
Author(s):  
J.A. Eades ◽  
A. van Dun
Keyword(s):  
Electron Microscope ◽  
Small Displacement ◽  
Private Communication ◽  
Piezoelectric Crystal ◽  
Double Exposure ◽  
Standard Methods ◽  
Elegant Method ◽  
The Right ◽  
Piezo Electric

The measurement of magnification in the electron microscope is always troublesome especially when a goniometer stage is in use, since there can be wide variations from calibrated values. One elegant method (L.M.Brown, private communication) of avoiding the difficulties of standard methods would be to fit a device which displaces the specimen a small but known distance and recording the displacement by a double exposure. Such a device would obviate the need for changing the specimen and guarantee that the magnification was measured under precisely the conditions used.Such a small displacement could be produced by any suitable transducer mounted in one of the specimen translation mechanisms. In the present case a piezoelectric crystal was used. Modern synthetic piezo electric ceramics readily give reproducible displacements in the right range for quite modest voltages (for example: Joyce and Wilson, 1969).

The Repair and Healing of Two Traumatic Ilial Body Fractures Adjacent to Cemented Total Hip Acetabular Components

1992 ◽  
Vol 05 (02) ◽  
pp. 85-89
Author(s):  
P. K. Shires ◽  
T. L. Dew
Keyword(s):  
Internal Fixation ◽  
Cancellous Bone ◽  
Surgical Techniques ◽  
Autogenous Bone ◽  
Cancellous Bone Graft ◽  
Standard Methods ◽  
Intravenous Antibiotics ◽  
Prophylactic Use ◽  
Acetabular Components ◽  
Autogenous Bone Grafts

SummaryThis report has documented the repair and healing of two ilial fractures in dogs which were compounded by the presence of a cemented acetabular prosthesis. While specific recommendations can not be made from such a small number of cases, the information presented herein suggests that such fractures will heal without complication if aseptic surgical techniques and standard methods of internal fixation are employed. The authors strongly recommend the prophylactic use of intravenous antibiotics and the placement of cancellous bone graft when the repair of such fractures is attempted.Two traumatic ilial fractures involving the cement/bone interface of acetabular prostheses were repaired using lag screws, cerclage wires, and autogenous bone grafts. The fractures healed and ambulatory function was regained.

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Enzyme System ◽  
Enzyme Kinetic ◽  
Clotting Time ◽  
Standard Methods

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

Non-standard methods for converting numbers from one number system to another

2020 ◽  
Vol 1 (9) ◽  
pp. 28-30
Author(s):  
D. M. Zlatopolski
Keyword(s):  
Binary System ◽  
Maximum Degree ◽  
Number System ◽  
Binary Form ◽  
Binary Number ◽  
Standard Methods ◽  
Optimal Variant ◽  
Decimal System ◽  
Large Numbers ◽  
Independent Work

The article describes a number of little-known methods for translating natural numbers from one number system to another. The first is a method for converting large numbers from the decimal system to the binary system, based on multiple divisions of a given number and all intermediate quotients by 64 (or another number equal to 2n ), followed by writing the last quotient and the resulting remainders in binary form. Then two methods of mutual translation of decimal and binary numbers are described, based on the so-called «Horner scheme». An optimal variant of converting numbers into the binary number system by the method of division by 2 is also given. In conclusion, a fragment of a manuscript from the beginning of the late 16th — early 17th centuries is published with translation into the binary system by the method of highlighting the maximum degree of number 2. Assignments for independent work of students are offered.

Interlaboratory Study of Standard Methods for Testing Multiwire Steel Prestressing Strand

PCI Journal ◽  
2020 ◽  
Vol 65 (4) ◽  
Author(s):  
Rémy D. Lequesne ◽  
William N. Collins ◽  
Enrico Lucon ◽  
David Darwin ◽  
Ashwin Poudel
Keyword(s):  
Interlaboratory Study ◽  
Standard Methods ◽  
Prestressing Strand

Flouride and Nitrate Status of Some Ground Waters in Chittor District of Rajasthan State (India)

2018 ◽  
Vol 6 (9) ◽  
pp. 119
Author(s):  
Trapti Sharma ◽  
R. P. Nagar ◽  
R. C. Gaur ◽  
Pooja Gupta ◽  
Charanjit Kaur
Keyword(s):  
Point Of View ◽  
World Health ◽  
Indian Council ◽  
Standard Methods ◽  
Ground Waters ◽  
Drinking Waters ◽  
Health Organization ◽  
Research World ◽  
Drinking Purpose

In Rajasthan state the ground waters of some areas like Ramganj-mandi, Morak, Barmer, Jaisalmer, Chittor and Udaipur etc. are susceptible from drinking point of view.To test the quality of groundwater in Chittor district 14, ground water samples were collected from various places and analyzed for pH, E.C., Fluoride and Nitrate parameters by standard methods (A.P.H. A., Washington, USA, 1995). The study revealed that none of the ground waters was found suitable completely from drinking point of view. Some are having electrical conductivity > 1.4 dS/m, some are having pH >8.5, some area having fluoride >1.5 ppm and some are having nitrate>45 ppm. These are the limits of various parameters permitted by various International authorities like Bureau of Indian Standard, Indian Council of Medical Research,world health Organization etc. for drinking waters. So, it is recommended to the residents of above areas to use water for drinking purpose only after reverse osmosis or adopting suitable method of removing excess of Fluoride and Nitrate for drinking water to avoid unwanted pathogenic diseases harmful for human health.

Evaluation of standard methods for collecting and processing fuel moisture samples

2016 ◽  
Author(s):  
Sally M. Haase ◽  
José Sánchez ◽  
David R. Weise
Keyword(s):  
Fuel Moisture ◽  
Standard Methods
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