Competitive ELISA

2010 ◽  
pp. 739-742 ◽  
Author(s):  
Torsten Meyer
Keyword(s):  
Competitive Elisa

scholarly journals Endocrine and Electrolyte Balances during Periovulatory Period in Cycling Mares

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 520 ◽  
Author(s):  
Katiuska Satué ◽  
Esterina Fazio ◽  
Ana Muñoz ◽  
Pietro Medica
Keyword(s):  
Estrous Cycle ◽  
Hpa Axis ◽  
Adrenocorticotropic Hormone ◽  
Venous Blood ◽  
Competitive Elisa ◽  
Direct Relation ◽  
Plasma Acth ◽  
Blood Samples ◽  
Hypothalamic Pituitary Adrenal ◽  
Stimulation Of

In cycling females, the periovulatory period is characterized by stimulation of the hypothalamic pituitary adrenal (HPA) axis. The aim of present study was to analyze the pattern and interrelationships among adrenocorticotropic hormone (ACTH), cortisol (CORT), aldosterone (ALD) and electrolytes (sodium—Na+, potassium—K+ and chloride—Cl−) during periovulatory period in cycling mares. Venous blood samples were obtained daily from a total of 23 Purebred Spanish broodmares, aged 7.09 ± 2.5 years, from day −5 to day +5 of estrous cycle, considering day 0, the day of ovulation. Plasma ACTH was measured by a fluorescent immunoassay kit, serum CORT and ALD by means of a competitive ELISA immunoassay, and plasma Na+, K+ and Cl− were quantified by an analyzer with selective electrodes for the three ions. ACTH showed higher concentrations at day 0 compared to days −5 to −1 and +1 to +3 (p < 0.05). CORT showed higher concentrations at day 0 compared to days −5 to −2 and +1 to +5 (p < 0.05). ALD showed higher concentrations at day 0 compared to days −5 to −2 (p < 0.05) and +2 (p < 0.05). Na+ and Cl− showed higher concentrations at day 0, compared to day −5 and +5. K+ showed lower concentrations at day 0 compared to day +1 (p < 0.05). The significant correlations obtained between ACTH and CORT (r = 0.20) and between ACTH and ALD (r = 0.32) suggest that although ACTH may have an effect both on CORT and ALD, there are other very important determinants that could be considered. Hence, it is possible to presume that the pituitary adrenocortical response and ALD may be involved in the ovulatory mechanisms without a direct relation with electrolyte pattern.

Serological evidence for Babesia canis infection of horses and an endemic focus of B. caballi in Hungary

2007 ◽  
Vol 55 (4) ◽  
pp. 491-500 ◽  
Author(s):  
S. Hornok ◽  
Renate Edelhofer ◽  
G. Földvári ◽  
Anja Joachim ◽  
R. Farkas
Keyword(s):  
Competitive Elisa ◽  
Babesia Canis ◽  
Age Group ◽  
Serological Evidence ◽  
Babesia Caballi ◽  
Seropositivity Rate ◽  
Blood Samples ◽  
Endemic Focus ◽  
Medium Level ◽  
Level 1

In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2–5 years. Babesia canis -specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating that the horses had previously been exposed to this piroplasm, but their infection must have been limited. The highest seropositivity rate was observed in the age group of 3–4 years, and males (stallions and geldings) were significantly more frequently infected than females. However, neither B. caballi nor B. canis could be identified in the peripheral blood samples of infected horses by PCR. Since most of the B. caballi -positive horses remained negative in the B. canis IFAT, whereas seroconversion solely to B. canis was detected in several regions of the country, serological cross-reaction between the two species can be discounted. This is the first serological evidence of horses being naturally infected with B. canis , supporting the view that piroplasms are less host specific than previously thought.

Amperometric Immunoassay of Azinphos‐Methyl in Water and Honeybees Based on Indirect Competitive ELISA

2008 ◽  
Vol 41 (3) ◽  
pp. 392-405 ◽  
Author(s):  
Alexey Ivanov ◽  
Gennady Evtugyn ◽  
Herman Budnikov ◽  
Stefano Girotti ◽  
Severino Ghini ◽  
...  
Keyword(s):  
Competitive Elisa ◽  
Indirect Competitive Elisa ◽  
Azinphos Methyl

Development of a competitive ELISA for the detection of soybean α subunit of β-conglycinin

2012 ◽  
Vol 47 (2) ◽  
pp. 280-287 ◽  
Author(s):  
Bin Liu ◽  
Da Teng ◽  
Yalin Yang ◽  
Xiumin Wang ◽  
Jianhua Wang
Keyword(s):  
Competitive Elisa ◽  
Α Subunit

Development of a competitive ELISA method based on VLPs detecting the antibodies of serotype A FMDV

2021 ◽  
pp. 114406
Author(s):  
Yun Zhang ◽  
Rui Wang ◽  
Manyuan Bai ◽  
Xuefei Wang ◽  
Hu Dong ◽  
...  
Keyword(s):  
Competitive Elisa ◽  
Elisa Method ◽  
Serotype A

Effectiveness of hermetic technologies in limiting aflatoxin B1 and fumonisin B1 contamination of stored maize grain under smallholder conditions in Zimbabwe

2018 ◽  
Vol 11 (3) ◽  
pp. 459-469 ◽  
Author(s):  
T.C. Murashiki ◽  
C. Chidewe ◽  
M.A. Benhura ◽  
L.R. Manema ◽  
B.M. Mvumi ◽  
...  
Keyword(s):  
High Performance ◽  
Fumonisin B1 ◽  
Competitive Elisa ◽  
Hermetic Storage ◽  
Rural Districts ◽  
Limit Of Quantitation ◽  
Significant Difference ◽  
Maize Grain ◽  
Storage Containers ◽  
Aflatoxin B

There is limited empirical evidence on the efficacy of hermetic storage containers in reducing mycotoxin occurrence in stored maize grain under smallholder field conditions. Levels of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) in maize samples collected from hermetic metal silos (148), hermetic grain bags (121) and conventional stores (179) during 2015 and 2016 storage seasons in two rural districts of Zimbabwe were assessed. AFB1 was determined using high performance liquid chromatography with post-column derivatisation and fluorescence detection, whilst FB1 was determined using direct competitive ELISA. All maize samples collected at harvest in 2015 and 2016 seasons contained FB1 at levels ranging from 10 to 462 μg/kg and 13 to 537 μg/kg, respectively. Use of hermetic containers did not seem to have any effect on the development of FB1 in stored maize grain, as there was no significant difference (P>0.05) in the increase of FB1 contamination in hermetic and conventional stores. Prior to storage, the levels of AFB1 in the maize ranged from below the limit of quantitation (LOQ) to 25.0 μg/kg, whilst levels during storage ranged from <LOQ to 8.60 μg/kg in hermetic silos, <LOQ to 8.37 μg/kg in hermetic bags and <LOQ to 791 μg/kg in conventional stores over the two storage seasons. The occurrence of AFB1 in maize stored in hermetic containers, was significantly (P<0.05) lower than that in conventional stores. Hermetic containers were more effective than conventional stores in limiting contamination of maize with AFB1 and subsequent human exposure to these toxins. Therefore, hermetic storage containers are recommended to smallholder maize producers for safe and effective limitation of AFB1 contamination during storage and hence reduce exposure among consumers.

scholarly journals Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 43 ◽  
Author(s):  
Alessandro Manenti ◽  
Agnieszka Katarzyna Maciola ◽  
Claudia Maria Trombetta ◽  
Otfried Kistner ◽  
Elisa Casa ◽  
...  
Keyword(s):  
Immune Responses ◽  
Competitive Elisa ◽  
Influenza Vaccines ◽  
Elisa Method ◽  
Serum Samples ◽  
Universal Vaccine ◽  
Administration Routes ◽  
Hemagglutinin Protein ◽  
Protective Antibodies ◽  
Universal Influenza Vaccine

Growing interest in universal influenza vaccines and novel administration routes has led to the development of alternative serological assays that are able to detect antibodies against conserved epitopes. We present a competitive ELISA method that is able to accurately determine the ratio of serum immunoglobulin G directed against the different domains of the hemagglutinin, the head and the stalk. Human serum samples were treated with two variants of the hemagglutinin protein from the A/California/7/2009 influenza virus. The signals detected were assigned to different groups of antibodies and presented as a ratio between head and stalk domains. A subset of selected sera was also tested by hemagglutination inhibition, single radial hemolysis, microneutralization, and enzyme-linked lectin assays. Pre-vaccination samples from adults showed a quite high presence of anti-stalk antibodies, and the results were substantially in line with those of the classical serological assays. By contrast, pre-vaccination samples from children did not present anti-stalk antibodies, and the majority of the anti-hemagglutinin antibodies that were detected after vaccination were directed against the head domain. The presented approach, when supported by further assays, can be used to assess the presence of specific anti-stalk antibodies and the potential boost of broadly protective antibodies, especially in the case of novel universal influenza vaccine approaches.

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