Towards Adaptable Networking: Defining the Protocol Optimization Architecture Requirements

2009 ◽  
pp. 282-291
Author(s):  
Martin André ◽  
Fumio Teraoka
Keyword(s):  
Protocol Optimization

scholarly journals Protocol optimization for simultaneous DNA and RNA co-extraction from single hard tick specimens

MethodsX ◽  
2021 ◽  
pp. 101315
Author(s):  
Alessandra Cafiso ◽  
Giulia Chiappa ◽  
Camilla Luzzago ◽  
Anita Koni ◽  
Daniele Bonato ◽  
...  
Keyword(s):  
Hard Tick ◽  
Dna And Rna ◽  
Protocol Optimization

scholarly journals Study and modulation of cardiac electroporation with a novel model utilizing human induced pluripotent stem cells

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
L Maizels ◽  
E Heller ◽  
M Landesberg ◽  
I Huber ◽  
G Arbel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Medical Center ◽  
Irreversible Electroporation ◽  
Public Institution ◽  
Cardiovascular Research ◽  
Protocol Optimization ◽  
Reversible Electroporation ◽  
Induced Pluripotent

Abstract Background Cardiac electroporation is a promising novel non-thermal ablation method, gaining significant interest with recent first-in-man data suggesting effective cardiac lesion generation with no collateral damage. Nevertheless, significant knowledge gaps exist regarding its electrophysiological consequences in cardiomyocytes, including; cell specificity, protocol optimization, irreversibility threshold, recovery time-constants, and the mechanistic nature of its cytolytic and anti-arrhythmic properties. Purpose Establishing an innovative in-vitro model for the study of cardiac electroporation-ablation, utilizing human induced pluripotent stem cells (hiPSCs). Methods and results Healthy-control hiPSC-derived cardiomyocytes were enzymatically dissociated and seeded as circular cell sheets (hiPSC-CCSs). Electroporation-ablation experiments were performed using a custom designed high-frequency electroporation (HF-EP) generator. Two needle-shaped electrodes were used for HF-EP delivery (Figure 1). Subsequently, detailed voltage- and Ca2+-mapping studies of the hiPSC-CCSs were conducted (Figure 2). HF-EP application resulted in the generation of electrically isolated lesions within the hiPSC-CCSs (Figure 3). Further characterization of the temporal changes and electrophysiological properties following electroporation revealed that; (1) lesions persisted over prolonged periods of time (days), indicating irreversible electroporation, (2) a temporal decrease in lesion dimensions was noted, consistent with a significant reversible electroporation component (Figures 3–5), (3) most tissue recovery had occurred within the first 15 minutes following electroporation, with little recovery beyond that time-frame, (4) increasing pulse-number augmented lesion area as well as the proportion of irreversible damage, and (5) electroporation sensitization was achieved by increasing extracellular Ca2+, indicating its crucial role in electroporation cytolysis, potentially via direct cellular toxicity and apoptosis facilitation (Figures 5–6). Finally, evaluating for HF-EP anti-arrhythmic properties, we targeted multiple rotors or focal triggered-activity generated in the hiPSC-CCSs. HF-EP application generated sustained line-blocks, isolating arrhythmogenic substrates within the hiPSC-CCSs while blocking the propagation of arrhythmic wavefronts (Figure 7). Conclusion Our results demonstrate the ability to study cardiac electroporation utilizing hiPSC-derived cardiomyocytes, provide novel insights into its temporal and electrophysiological characteristics, facilitate electroporation protocol optimization, screen for potential electroporation sensitizers, and to study its mechanistic nature and anti-arrhythmic properties. FUNDunding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): Division of Cardiology, and Tamman Cardiovascular Research Institute, Leviev Heart Center, Sheba Medical Center - Tel Hashomer, Ramat-Gan, Israel Figures 1–4 Figures 5–7

Spiral 3D time-of-flight MR angiography for rapid non-contrast carotid artery imaging: Clinical feasibility and protocol optimization

2022 ◽  
Vol 93 ◽  
pp. 20-28
Author(s):  
Elisabeth Sartoretti ◽  
Sabine Sartoretti-Schefer ◽  
Luuk van Smoorenburg ◽  
Christoph A. Binkert ◽  
Andreas Gutzeit ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Mr Angiography ◽  
Time Of Flight ◽  
Protocol Optimization

Communication protocol optimization for enhanced GPU performance

2020 ◽  
Vol 64 (3/4) ◽  
pp. 9:1-9:9
Author(s):  
S. S. Sharkawi ◽  
G. A. Chochia
Keyword(s):  
Communication Protocol ◽  
Protocol Optimization

A Compiler-Based Approach To Protocol Optimization

2005 ◽  
Author(s):  
C. Castelluccia ◽  
P. Hoschka
Keyword(s):  
Protocol Optimization

High-Intensity Interval Exercise in Chronic Heart Failure: Protocol Optimization

2012 ◽  
Vol 18 (2) ◽  
pp. 126-133 ◽  
Author(s):  
Philippe Meyer ◽  
Eve Normandin ◽  
Mathieu Gayda ◽  
Guillaume Billon ◽  
Thibaut Guiraud ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
High Intensity ◽  
Interval Exercise ◽  
Protocol Optimization ◽  
High Intensity Interval
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