Impedimetric Detection of Transient Receptor Potential (TRP) Channel Activity

2009 ◽  
pp. 169-172
Author(s):  
O. Pänke ◽  
A. A. Robitzki
Keyword(s):  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Trp Channel ◽  
Channel Activity ◽  
Transient Receptor

scholarly journals Expression of transient receptor potential (TRP) channel mRNAs in the mouse olfactory bulb

2012 ◽  
Vol 524 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Hong-Wei Dong ◽  
James C. Davis ◽  
ShengYuan Ding ◽  
Qiang Nai ◽  
Fu-Ming Zhou ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Trp Channel ◽  
Transient Receptor

scholarly journals Molecular Bases of Multimodal Regulation of a Fungal Transient Receptor Potential (TRP) Channel

2013 ◽  
Vol 288 (21) ◽  
pp. 15303-15317 ◽  
Author(s):  
Makoto Ihara ◽  
Shin Hamamoto ◽  
Yohei Miyanoiri ◽  
Mitsuhiro Takeda ◽  
Masatsune Kainosho ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Trp Channel ◽  
Transient Receptor ◽  
Molecular Bases

A cell-based impedance assay for monitoring transient receptor potential (TRP) ion channel activity

2011 ◽  
Vol 26 (5) ◽  
pp. 2376-2382 ◽  
Author(s):  
Oliver Pänke ◽  
Winnie Weigel ◽  
Sabine Schmidt ◽  
Anja Steude ◽  
Andrea A. Robitzki
Keyword(s):  
Ion Channel ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Channel Activity ◽  
A Cell ◽  
Transient Receptor ◽  
Trp Ion Channel

scholarly journals TRP Channels as Sensors of Aldehyde and Oxidative Stress

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1401
Author(s):  
Katharina E. M. Hellenthal ◽  
Laura Brabenec ◽  
Eric R. Gross ◽  
Nana-Maria Wagner
Keyword(s):  
Lipid Peroxidation ◽  
Transient Receptor Potential ◽  
Trp Channels ◽  
Treatment Options ◽  
Receptor Potential ◽  
The Body ◽  
Trp Channel ◽  
Alcoholic Beverages ◽  
Organ Injury ◽  
Transient Receptor

The transient receptor potential (TRP) cation channel superfamily comprises more than 50 channels that play crucial roles in physiological processes. TRP channels are responsive to several exogenous and endogenous biomolecules, with aldehydes emerging as a TRP channel trigger contributing to a cellular cascade that can lead to disease pathophysiology. The body is not only exposed to exogenous aldehydes via tobacco products or alcoholic beverages, but also to endogenous aldehydes triggered by lipid peroxidation. In response to lipid peroxidation from inflammation or organ injury, polyunsaturated fatty acids undergo lipid peroxidation to aldehydes, such as 4-hydroxynonenal. Reactive aldehydes activate TRP channels via aldehyde-induced protein adducts, leading to the release of pro-inflammatory mediators driving the pathophysiology caused by cellular injury, including inflammatory pain and organ reperfusion injury. Recent studies have outlined how aldehyde dehydrogenase 2 protects against aldehyde toxicity through the clearance of toxic aldehydes, indicating that targeting the endogenous aldehyde metabolism may represent a novel treatment strategy. An addition approach can involve targeting specific TRP channel regions to limit the triggering of a cellular cascade induced by aldehydes. In this review, we provide a comprehensive summary of aldehydes, TRP channels, and their interactions, as well as their role in pathological conditions and the different therapeutical treatment options.

scholarly journals Functional characterization of transient receptor potential channels in mouse urothelial cells

2010 ◽  
Vol 298 (3) ◽  
pp. F692-F701 ◽  
Author(s):  
Wouter Everaerts ◽  
Joris Vriens ◽  
Grzegorz Owsianik ◽  
Giovanni Appendino ◽  
Thomas Voets ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Patch Clamp ◽  
Calcium Imaging ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Functional Expression ◽  
Sensory Function ◽  
Trp Channel ◽  
Urothelial Cells ◽  
Transient Receptor

The bladder urothelium is currently believed to be a sensory structure, contributing to mechano- and chemosensation in the bladder. Transient receptor potential (TRP) cation channels act as polymodal sensors and may underlie some of the receptive properties of urothelial cells. However, the exact TRP channel expression profile of urothelial cells is unclear. In this study, we have performed a systematic analysis of the molecular and functional expression of various TRP channels in mouse urothelium. Urothelial cells from control and trpv4−/− mice were isolated, cultured (12–48 h), and used for quantitative real-time PCR, immunocytochemistry, calcium imaging, and whole cell patch-clamp experiments. At the mRNA level, TRPV4, TRPV2, and TRPM7 were the most abundantly expressed TRP genes. Immunohistochemistry showed a clear expression of TRPV4 in the plasma membrane, whereas TRPV2 was more prominent in the cytoplasm. TRPM7 was detected in the plasma membrane as well as cytoplasmic vesicles. Calcium imaging and patch-clamp experiments using TRP channel agonists and antagonists provided evidence for the functional expression of TRPV4, TRPV2, and TRPM7 but not of TRPA1, TRPV1, and TRPM8. In conclusion, we have demonstrated functional expression of TRPV4, TRPV2, and TRPM7 in mouse urothelial cells. These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction.

scholarly journals Responses to transient receptor potential (TRP) channel agonists in Chlamydomonas reinhardtii

Biology Open ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. bio053140
Author(s):  
Mamoru Wada ◽  
Itaru Kaizuka ◽  
Kenjiro Yoshimura
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Trp Channel ◽  
Transient Receptor

scholarly journals Upregulation of Transient Receptor Potential Vanilloid Type-1 Channel Activity and Ca2+Influx Dysfunction in Human Pterygial Cells

2016 ◽  
Vol 57 (6) ◽  
pp. 2564 ◽  
Author(s):  
Fabian Garreis ◽  
Antje Schröder ◽  
Peter S. Reinach ◽  
Stefanie Zoll ◽  
Noushafarin Khajavi ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Channel Activity ◽  
Transient Receptor

scholarly journals Human transient receptor potential (TRP) channel expression profiling in carcinogenesis

2015 ◽  
Vol 59 (7-8-9) ◽  
pp. 399-406 ◽  
Author(s):  
Michela Bernardini ◽  
Alessandra Fiorio Pla ◽  
Natalia Prevarskaya ◽  
Dimitra Gkika
Keyword(s):  
Expression Profiling ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Trp Channel ◽  
Channel Expression ◽  
Transient Receptor
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