Human transient receptor potential (TRP) channel expression profiling in carcinogenesis

AbstractTransient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal–fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5–E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.

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Faculty Opinions recommendation of Emergence of functional sensory subtypes as defined by transient receptor potential channel expression.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1070870.523819 ◽

2007 ◽

Author(s):

Michaela Kress

Keyword(s):

Transient Receptor Potential ◽

Receptor Potential ◽

Transient Receptor Potential Channel ◽

Channel Expression ◽

Transient Receptor

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Expression of transient receptor potential (TRP) channel mRNAs in the mouse olfactory bulb

Neuroscience Letters ◽

10.1016/j.neulet.2012.07.013 ◽

2012 ◽

Vol 524 (1) ◽

pp. 49-54 ◽

Cited By ~ 13

Author(s):

Hong-Wei Dong ◽

James C. Davis ◽

ShengYuan Ding ◽

Qiang Nai ◽

Fu-Ming Zhou ◽

...

Keyword(s):

Olfactory Bulb ◽

Transient Receptor Potential ◽

Receptor Potential ◽

Trp Channel ◽

Transient Receptor

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Molecular Bases of Multimodal Regulation of a Fungal Transient Receptor Potential (TRP) Channel

Journal of Biological Chemistry ◽

10.1074/jbc.m112.434795 ◽

2013 ◽

Vol 288 (21) ◽

pp. 15303-15317 ◽

Cited By ~ 11

Author(s):

Makoto Ihara ◽

Shin Hamamoto ◽

Yohei Miyanoiri ◽

Mitsuhiro Takeda ◽

Masatsune Kainosho ◽

...

Keyword(s):

Transient Receptor Potential ◽

Receptor Potential ◽

Trp Channel ◽

Transient Receptor ◽

Molecular Bases

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TRP Channels as Sensors of Aldehyde and Oxidative Stress

Biomolecules ◽

10.3390/biom11101401 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1401

Author(s):

Katharina E. M. Hellenthal ◽

Laura Brabenec ◽

Eric R. Gross ◽

Nana-Maria Wagner

Keyword(s):

Lipid Peroxidation ◽

Transient Receptor Potential ◽

Trp Channels ◽

Treatment Options ◽

Receptor Potential ◽

The Body ◽

Trp Channel ◽

Alcoholic Beverages ◽

Organ Injury ◽

Transient Receptor

The transient receptor potential (TRP) cation channel superfamily comprises more than 50 channels that play crucial roles in physiological processes. TRP channels are responsive to several exogenous and endogenous biomolecules, with aldehydes emerging as a TRP channel trigger contributing to a cellular cascade that can lead to disease pathophysiology. The body is not only exposed to exogenous aldehydes via tobacco products or alcoholic beverages, but also to endogenous aldehydes triggered by lipid peroxidation. In response to lipid peroxidation from inflammation or organ injury, polyunsaturated fatty acids undergo lipid peroxidation to aldehydes, such as 4-hydroxynonenal. Reactive aldehydes activate TRP channels via aldehyde-induced protein adducts, leading to the release of pro-inflammatory mediators driving the pathophysiology caused by cellular injury, including inflammatory pain and organ reperfusion injury. Recent studies have outlined how aldehyde dehydrogenase 2 protects against aldehyde toxicity through the clearance of toxic aldehydes, indicating that targeting the endogenous aldehyde metabolism may represent a novel treatment strategy. An addition approach can involve targeting specific TRP channel regions to limit the triggering of a cellular cascade induced by aldehydes. In this review, we provide a comprehensive summary of aldehydes, TRP channels, and their interactions, as well as their role in pathological conditions and the different therapeutical treatment options.

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Functional characterization of transient receptor potential channels in mouse urothelial cells

AJP Renal Physiology ◽

10.1152/ajprenal.00599.2009 ◽

2010 ◽

Vol 298 (3) ◽

pp. F692-F701 ◽

Cited By ~ 101

Author(s):

Wouter Everaerts ◽

Joris Vriens ◽

Grzegorz Owsianik ◽

Giovanni Appendino ◽

Thomas Voets ◽

...

Keyword(s):

Plasma Membrane ◽

Patch Clamp ◽

Calcium Imaging ◽

Transient Receptor Potential ◽

Receptor Potential ◽

Functional Expression ◽

Sensory Function ◽

Trp Channel ◽

Urothelial Cells ◽

Transient Receptor

The bladder urothelium is currently believed to be a sensory structure, contributing to mechano- and chemosensation in the bladder. Transient receptor potential (TRP) cation channels act as polymodal sensors and may underlie some of the receptive properties of urothelial cells. However, the exact TRP channel expression profile of urothelial cells is unclear. In this study, we have performed a systematic analysis of the molecular and functional expression of various TRP channels in mouse urothelium. Urothelial cells from control and trpv4−/− mice were isolated, cultured (12–48 h), and used for quantitative real-time PCR, immunocytochemistry, calcium imaging, and whole cell patch-clamp experiments. At the mRNA level, TRPV4, TRPV2, and TRPM7 were the most abundantly expressed TRP genes. Immunohistochemistry showed a clear expression of TRPV4 in the plasma membrane, whereas TRPV2 was more prominent in the cytoplasm. TRPM7 was detected in the plasma membrane as well as cytoplasmic vesicles. Calcium imaging and patch-clamp experiments using TRP channel agonists and antagonists provided evidence for the functional expression of TRPV4, TRPV2, and TRPM7 but not of TRPA1, TRPV1, and TRPM8. In conclusion, we have demonstrated functional expression of TRPV4, TRPV2, and TRPM7 in mouse urothelial cells. These channels may contribute to the (mechano)sensory function of the urothelial layer and represent potential targets for the treatment of bladder dysfunction.

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