Gene Therapy for Bone Tissue Engineering

2014 ◽  
pp. 33-53
Author(s):  
Yu-Chen Hu
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Bone tissue engineering and repair by gene therapy

10.2741/2724 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 833 ◽  
Author(s):  
Volker, M. Betz
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 29 (2) ◽  
pp. 206-220 ◽  
Author(s):  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J.A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

scholarly journals Gene therapy for bone tissue engineering

2016 ◽  
Vol 13 (2) ◽  
pp. 111-125 ◽  
Author(s):  
Young-Dong Kim ◽  
Prasad Pofali ◽  
Tae-Eun Park ◽  
Bijay Singh ◽  
Kihyun Cho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Porous bioprinted constructs in BMP-2 non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 1 (48) ◽  
pp. 6619 ◽  
Author(s):  
Loek D. Loozen ◽  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J. A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

scholarly journals Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Asghar Fallah ◽  
Mahdieh Alipour ◽  
Zahra Jamali ◽  
Akbar Farjadfar ◽  
Leila Roshangar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Gene Therapy ◽  
Alkaline Phosphatase ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Wharton’S Jelly ◽  
Alizarin Red ◽  
Wharton's Jelly ◽  
Study Results

Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s jelly-derived stem cells (WJSCs). For this purpose, human BMP2 and miR-424 DNA codes were cloned in the third generation of lentiviral vectors and then used for HEK-293T cell transfection. Lentiviral plasmids contained miR424, BMP-2, miR424-BMP2, green fluorescent protein (GFP) genes, and helper vectors. The recombinant lentiviral particles transduced the WJSCs, and the osteogenesis was evaluated by real-time PCR, Western blot, Alizarin Red staining, and alkaline phosphatase enzyme activity. According to the results, there was a significant increase in the expression of the BMP2 gene and secretion of Osteocalcin protein in the group of miR424-BMP2. Moreover, the amount of dye deposition in Alizarin Red staining and alkaline phosphatase activity was significantly higher in the mentioned group ( p < 0.05 ). Thus, the current study results clarify the efficacy of gene therapy by miR424-BMP2 vectors for bone tissue engineering. These data could help guide the development of gene therapy-based protocols for bone tissue engineering.

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