Gene Therapy Strategies in Bone Tissue Engineering and Current Clinical Applications

2018 ◽  
pp. 85-101 ◽  
Author(s):  
Aysegul Atasoy-Zeybek ◽  
Gamze Torun Kose
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Clinical Applications ◽  
Therapy Strategies

Bone tissue engineering and repair by gene therapy

10.2741/2724 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 833 ◽  
Author(s):  
Volker, M. Betz
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 29 (2) ◽  
pp. 206-220 ◽  
Author(s):  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J.A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

scholarly journals Gene therapy for bone tissue engineering

2016 ◽  
Vol 13 (2) ◽  
pp. 111-125 ◽  
Author(s):  
Young-Dong Kim ◽  
Prasad Pofali ◽  
Tae-Eun Park ◽  
Bijay Singh ◽  
Kihyun Cho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Porous bioprinted constructs in BMP-2 non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 1 (48) ◽  
pp. 6619 ◽  
Author(s):  
Loek D. Loozen ◽  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J. A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

scholarly journals Bioinspired Modifications of PEEK Implants for Bone Tissue Engineering

2021 ◽  
Vol 8 ◽  
Author(s):  
Xinming Gu ◽  
Xiaolin Sun ◽  
Yue Sun ◽  
Jia Wang ◽  
Yiping Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Elastic Modulus ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Clinical Applications ◽  
Structure And Function ◽  
Natural Bone ◽  
The Future ◽  
And Function ◽  
Surrounding Bone

In recent years, polyetheretherketone (PEEK) has been increasingly employed as an implant material in clinical applications. Although PEEK is biocompatible, chemically stable, and radiolucent and has an elastic modulus similar to that of natural bone, it suffers from poor integration with surrounding bone tissue after implantation. To improve the bioactivity of PEEK, numerous strategies for functionalizing the PEEK surface and changing the PEEK structure have been proposed. Inspired by the components, structure, and function of bone tissue, this review discusses strategies to enhance the biocompatibility of PEEK implants and provides direction for fabricating multifunctional implants in the future.

scholarly journals Overexpression Effects of miR-424 and BMP2 on the Osteogenesis of Wharton’s Jelly-Derived Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Asghar Fallah ◽  
Mahdieh Alipour ◽  
Zahra Jamali ◽  
Akbar Farjadfar ◽  
Leila Roshangar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Gene Therapy ◽  
Alkaline Phosphatase ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Wharton’S Jelly ◽  
Alizarin Red ◽  
Wharton's Jelly ◽  
Study Results

Recently, the translational application of noncoding RNAs is accelerated dramatically. In this regard, discovering therapeutic roles of microRNAs by developing synthetic RNA and vector-based RNA is attracting attention. Here, we studied the effect of BMP2 and miR-424 on the osteogenesis of Wharton’s jelly-derived stem cells (WJSCs). For this purpose, human BMP2 and miR-424 DNA codes were cloned in the third generation of lentiviral vectors and then used for HEK-293T cell transfection. Lentiviral plasmids contained miR424, BMP-2, miR424-BMP2, green fluorescent protein (GFP) genes, and helper vectors. The recombinant lentiviral particles transduced the WJSCs, and the osteogenesis was evaluated by real-time PCR, Western blot, Alizarin Red staining, and alkaline phosphatase enzyme activity. According to the results, there was a significant increase in the expression of the BMP2 gene and secretion of Osteocalcin protein in the group of miR424-BMP2. Moreover, the amount of dye deposition in Alizarin Red staining and alkaline phosphatase activity was significantly higher in the mentioned group ( p < 0.05 ). Thus, the current study results clarify the efficacy of gene therapy by miR424-BMP2 vectors for bone tissue engineering. These data could help guide the development of gene therapy-based protocols for bone tissue engineering.

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