DNA-probing as a tool to monitor the distribution of N2-fixing, denitrifying and nitrifying bacteria in soils

1997 ◽  
pp. 195-198
Author(s):  
H. Bothe ◽  
K. Kloos ◽  
K. Kaiser ◽  
U. Hüsgen ◽  
S. Sonnwald ◽  
...  
Keyword(s):  
Nitrifying Bacteria

Domestication of Halotolerant Heterotrophic Nitrifying Bacteria and Identification of Two Isolated Strains*

2011 ◽  
Vol 17 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Peiyu ZHANG ◽  
Yang QU ◽  
Ruixia YANG ◽  
Shasha GUO ◽  
Deshuang YU
Keyword(s):  
Nitrifying Bacteria ◽  
Heterotrophic Nitrifying Bacteria

Distribution of nitrifying bacteria in a shallow stream

1997 ◽  
Vol 36 (8-9) ◽  
pp. 161-166 ◽  
Author(s):  
Ivana Jancarkova ◽  
Tove A. Larsen ◽  
Willi Gujer
Keyword(s):  
Field Experiments ◽  
Heterotrophic Bacteria ◽  
Pollution Source ◽  
Nitrifying Bacteria ◽  
Channel Water ◽  
River Bed ◽  
Hydraulic Conditions ◽  
Major Floods ◽  
Shallow Stream ◽  
Stream Bed

A project investigating the dynamics of self-purification processes in a shallow stream is carried out. Effects of the concentration gradient due to the distance to the pollution source, of hydraulic conditions in the river bed and of storm floods on the distribution of nitrifying bacteria were studied with the help of laboratory and field experiments. Nitrifiers density on the surface of the stream bed increased rapidly up to a distance of 300 m from the WWTP indicating possible competition of the nitrifiers with the heterotrophic bacteria close to the WWTP. Afterwards a slight decrease in the downstream direction was observed. In vertical profiles, higher bacterial densities were found at sites with rapid infiltration of channel water to the stream bed than at sites with no exchange between channel water and stream bed water or where stream bed water exfiltrated. A major flood event scoured the nitrifiers nearly totally from the surface of the river bed. Major floods belong so to the most dominant processes controlling self-purification in shallow streams. Minor floods, however, don't scour bacteria in the depth of the stream bed that could then be important for the self-purification processes.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

scholarly journals Microfiber structure for enhanced immobilization of nitrifying bacteria in a post-nitrification reactor

2021 ◽  
Vol 21 ◽  
pp. 101373
Author(s):  
Lucie Svobodová ◽  
Karel Havlíček ◽  
Magda Nechanická ◽  
Roman Špánek ◽  
Brigita Kolčavová Sirková ◽  
...  
Keyword(s):  
Nitrifying Bacteria

The effect of 2-chloro, 6-(trichloromethyl) pyridine on the chemoautotrophic metabolism of nitrifying bacteria

1965 ◽  
Vol 31 (1) ◽  
pp. 137-144 ◽  
Author(s):  
N. E. R. Campbell ◽  
M. I. H. Aleem
Keyword(s):  
Nitrifying Bacteria

Investigation of aerobic and anaerobic ammonium-oxidising bacteria presence in a small full-scale wastewater treatment system comprised by UASB reactor and three polishing ponds

2010 ◽  
Vol 61 (3) ◽  
pp. 737-743 ◽  
Author(s):  
J. C. Araujo ◽  
M. M. S. Correa ◽  
E. C. Silva ◽  
A. P. Campos ◽  
V. M. Godinho ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Ammonia Removal ◽  
Treatment System ◽  
Nitrifying Bacteria ◽  
Uasb Reactor ◽  
Anammox Bacteria ◽  
Fluorescence In Situ Hybridisation ◽  
Probable Number ◽  
Sediment Samples ◽  
Wastewater Treatment System

This work applied PCR amplification method and Fluorescence in situ hybridisation (FISH) with primers and probes specific for the anammox organisms and aerobic ammonia-oxidising β-Proteobacteria in order to detect these groups in different samples from a wastewater treatment system comprised by UASB reactor and three polishing (maturation) ponds in series. Seven primer pairs were used in order to detect Anammox bacteria. Positive results were obtained with three of them, suggesting that Anammox could be present in polishing pond sediments. However, Anammox bacteria were not detected by FISH, indicating that they were not present in sediment samples, or they could be present but below FISH detection limit. Aerobic ammonia- and nitrite-oxidising bacteria were verified in water column samples through Most Probable Number (MPN) analysis, but they were not detected in sediment samples by FISH. Ammonia removal efficiencies occurred systematically along the ponds (24, 32, and 34% for polishing pond 1, 2, and 3, respectively) but the major reaction responsible for this removal is still unclear. Some nitrification might have occurred in water samples because some nitrifying bacteria were present. Also Anammox reaction might have occurred because Anammox genes were detected in the sediments, but probably this reaction was too low to be noticed. It is important also to consider that some of the ammonia removal observed might be related to NH3 stripping, associated with the pH increase resulting from the intensive photosynthetic activity in the ponds (mechanism under investigation). Therefore, it can be concluded that more than one mechanism (or reaction) might be involved in the ammonia removal in the polishing ponds investigated in this study.

Marine and Terrestrial Nitrifying Bacteria are Sources of Diverse Bacteriohopanepolyols

2021 ◽  
Author(s):  
F.J. Elling ◽  
T.W. Evans ◽  
J.D. Hemingway ◽  
J.J. Kharbush ◽  
V. Nathan ◽  
...  
Keyword(s):  
Nitrifying Bacteria

scholarly journals The Influence of Mineral Fertilizer Combined With a Nitrification Inhibitor on Microbial Populations and Activities in Calcareous Uzbekistanian Soil Under Cotton Cultivation

2001 ◽  
Vol 1 ◽  
pp. 108-113 ◽  
Author(s):  
Dilfuza Egamberdiyeva ◽  
Muhiddin Mamiev ◽  
Svetlana K. Poberejskaya
Keyword(s):  
Nitrification Inhibitor ◽  
Mineral Fertilizer ◽  
Denitrifying Bacteria ◽  
Nitrifying Bacteria ◽  
Most Probable Number ◽  
Nitrification Rate ◽  
Nitrification Inhibitors ◽  
Probable Number ◽  
Soil Microbial ◽  
Soil Microbial Population

Application of fertilizers combined with nitrification inhibitors affects soil microbial biomass and activity. The objective of this research was to determine the effects of fertilizer application combined with the nitrification inhibitor potassium oxalate (PO) on soil microbial population and activities in nitrogen-poor soil under cotton cultivation in Uzbekistan. Fertilizer treatments were N as urea, P as ammophos, and K as potassium chloride. The nitrification inhibitor PO was added to urea and ammophos at the rate of 2%. Three treatments—N200P140K60(T1), N200P140 POK60(T2), and N200P140 POK60(T3) mg kg-1soil—were applied for this study. The control (C) was without fertilizer and PO. The populations of oligotrophic bacteria, ammonifying bacteria, nitrifying bacteria, denitrifying bacteria, mineral assimilating bacteria, oligonitrophilic bacteria, and bacteria group Azotobacter were determined by the most probable number method. The treatments T2 and T3 increased the number of oligonitrophilic bacteria and utilization mineral forms of nitrogen on the background of reducing number of ammonifying bacteria. T2 and T3 also decreased the number of nitrifying bacteria, denitrifying bacteria, and net nitrification. In conclusion, our experiments showed that PO combined with mineral fertilizer is one of the most promising compounds for inhibiting nitrification rate, which was reflected in the increased availability and efficiency of fertilizer nitrogen to the cotton plants. PO combined with mineral fertilizer has no negative effects on nitrogen-fixing bacteria Azotobacter and oligo-nitrophilic bacteria.

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