Investigation of aerobic and anaerobic ammonium-oxidising bacteria presence in a small full-scale wastewater treatment system comprised by UASB reactor and three polishing ponds

2010 ◽  
Vol 61 (3) ◽  
pp. 737-743 ◽  
Author(s):  
J. C. Araujo ◽  
M. M. S. Correa ◽  
E. C. Silva ◽  
A. P. Campos ◽  
V. M. Godinho ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Ammonia Removal ◽  
Treatment System ◽  
Nitrifying Bacteria ◽  
Uasb Reactor ◽  
Anammox Bacteria ◽  
Fluorescence In Situ Hybridisation ◽  
Probable Number ◽  
Sediment Samples ◽  
Wastewater Treatment System

This work applied PCR amplification method and Fluorescence in situ hybridisation (FISH) with primers and probes specific for the anammox organisms and aerobic ammonia-oxidising β-Proteobacteria in order to detect these groups in different samples from a wastewater treatment system comprised by UASB reactor and three polishing (maturation) ponds in series. Seven primer pairs were used in order to detect Anammox bacteria. Positive results were obtained with three of them, suggesting that Anammox could be present in polishing pond sediments. However, Anammox bacteria were not detected by FISH, indicating that they were not present in sediment samples, or they could be present but below FISH detection limit. Aerobic ammonia- and nitrite-oxidising bacteria were verified in water column samples through Most Probable Number (MPN) analysis, but they were not detected in sediment samples by FISH. Ammonia removal efficiencies occurred systematically along the ponds (24, 32, and 34% for polishing pond 1, 2, and 3, respectively) but the major reaction responsible for this removal is still unclear. Some nitrification might have occurred in water samples because some nitrifying bacteria were present. Also Anammox reaction might have occurred because Anammox genes were detected in the sediments, but probably this reaction was too low to be noticed. It is important also to consider that some of the ammonia removal observed might be related to NH3 stripping, associated with the pH increase resulting from the intensive photosynthetic activity in the ponds (mechanism under investigation). Therefore, it can be concluded that more than one mechanism (or reaction) might be involved in the ammonia removal in the polishing ponds investigated in this study.

Performance evaluation of a simple wastewater treatment system comprised by UASB reactor, shallow polishing ponds and coarse rock filter

2008 ◽  
Vol 58 (6) ◽  
pp. 1313-1319 ◽  
Author(s):  
Marcos von Sperling ◽  
Carolina Moreira Oliveira ◽  
Juliana G. B. Andrada ◽  
Valéria M. Godinho ◽  
Fernando A. L. Assunção ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Ammonia Removal ◽  
Treatment System ◽  
Uasb Reactor ◽  
E Coli ◽  
Wastewater Treatment System ◽  
Treatment Processes ◽  
Natural Treatment ◽  
In Series ◽  
Overall Performance

The work investigates a small full-scale wastewater treatment system comprised by the following units in series: UASB reactor, three polishing ponds and one coarse rock filter. The overall performance of the system is analyzed based on three years of monitoring using physical-chemical and biological parameters. Good organic matter, suspended solids and ammonia removal is achieved, together with excellent coliform removal (5.70 log units). Mean effluent concentrations of the main parameters are: BOD: 39 mg/L; COD: 109 mg/L; SS = 41 mg/L; ammonia: 10 mg/L; E. coli: 540 MPN/100 mL, indicating compliance with many regulations for effluent discharge and reuse. Main algal classes found in the ponds and final effluent were chlorophyta and euglenophyta. The system is completely unmechanized and has a relatively small total hydraulic retention time (less than 13 days), compared with most natural treatment processes. No sludge removal from the ponds and filter has been necessary so far.

Activities and Metabolic Versatility of Distinct Anammox Bacteria in a Full-Scale Wastewater Treatment System

2021 ◽  
pp. 117763
Author(s):  
Yuchun Yang ◽  
Mohammad Azari ◽  
Craig W. Herbold ◽  
Meng Li ◽  
Huaihai Chen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Full Scale ◽  
Treatment System ◽  
Anammox Bacteria ◽  
Wastewater Treatment System ◽  
Metabolic Versatility

Enrichment of nitrifying microbial communities from shrimp farms and commercial inocula

2003 ◽  
Vol 48 (8) ◽  
pp. 143-150 ◽  
Author(s):  
C. Paungfoo ◽  
P. Prasertsan ◽  
N. Intrasungkha ◽  
L.L. Blackall ◽  
R. Bhamidimarri
Keyword(s):  
In Situ Hybridisation ◽  
Ammonia Removal ◽  
Microbial Consortia ◽  
Nitrifying Bacteria ◽  
Most Probable Number ◽  
Ammonia Oxidizing Bacteria ◽  
Fluorescence In Situ Hybridisation ◽  
Probable Number ◽  
Shrimp Farm ◽  
Commercial Seed

Nitrifying bacteria were selected from shrimp farm water and sediment (ÒnaturalÓ seed) in Thailand and from commercial seed cultures. The microbial consortia from each source giving the best ammonia removal during batch culture pre-enrichments were used as inocula for two sequencing batch reactors (SBRs). Nitrifiers were cultivated in the SBRs with 100 mg NH4-N/l and artificial wastewater containing 25 ppt salinity. The two SBRs were operated at a 7 d hydraulic retention time (HRT) for 77 d after which the HRT was reduced to 3.5 d. The amounts of ammonia removed from the influent by microorganisms sourced from the natural seed were 85% and 92% for the 7 d HRT and the 3.5 d HRT, respectively. The ammonia removals of microbial consortia from the commercial seed were 71% and 83% for these HRTs respectively. The quantity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) was determined in the SBRs using the most probable number (MPN) technique. Both AOB and NOB increased in number over the long-term operation of both SBRs. According to quantitative fluorescence in situ hybridisation (FISH) probing, AOB from the natural seed and commercial seed comprised 21 ± 2% and 30 ± 2%, respectively of all bacteria. NOB could not be detected with currently-reported FISH probes, suggesting that novel NOB were enriched from both sources. Taken collectively, the results from this study provide an indication that the nitrifiers from shrimp farm sources are more effective at ammonia removal than those from commercial seed cultures.

scholarly journals Anammox Bacteria Are Potentially Involved in Anaerobic Ammonium Oxidation Coupled to Iron(III) Reduction in the Wastewater Treatment System

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao-Ru Yang ◽  
Hu Li ◽  
Jian-Qiang Su ◽  
Guo-Wei Zhou
Keyword(s):  
Wastewater Treatment ◽  
Amplicon Sequencing ◽  
Anaerobic Ammonium Oxidation ◽  
Nitrite Reduction ◽  
Treatment System ◽  
Anammox Bacteria ◽  
Rrna Gene ◽  
Ammonium Oxidation ◽  
Wastewater Treatment System ◽  
Relative Abundances

Anaerobic ammonium oxidation coupled to nitrite reduction (termed as Anammox) was demonstrated as an efficient pathway to remove nitrogen from a wastewater treatment system. Recently, anaerobic ammonium oxidation was also identified to be linked to iron(III) reduction (termed Feammox) with dinitrogen, nitrite, or nitrate as end-product, reporting to enhance nitrogen removal from the wastewater treatment system. However, little is known about the role of Anammox bacteria in the Feammox process. Here, slurry from wastewater reactor amended with ferrihydrite was employed to investigate activity of Anammox bacteria in the Feammox process using the 15N isotopic tracing technique combined with 16S rRNA gene amplicon sequencing. A significantly positive relationship between rates of 15N2 production and iron(III) reduction indicated the occurrence of Feammox during incubation. Relative abundances of Anammox bacteria including Brocadia, Kuenenia, Jettenia, and unclassified Brocadiaceae were detected with low relative abundances, whereas Geobacteraceae dominated in the treatment throughout the incubation. 15N2 production rates significantly positively correlated with relative abundances of Geobacter, unclassified Geobacteraceae, and Anammox bacteria, revealing their contribution to nitrogen generation via Feammox. Overall, these findings suggested Anammox bacteria or cooperation between Anammox bacteria and iron(III) reducers serves a potential role in Feammox process.

Performance of hybrid small wastewater treatment system consisting of jet mixed separator and rotating biological contactor

1997 ◽  
Vol 35 (6) ◽  
pp. 63-70 ◽  
Author(s):  
Yoshimasa Watanabe ◽  
Yoshihiko Iwasaki
Keyword(s):  
Wastewater Treatment ◽  
Electric Power ◽  
Hybrid System ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Treatment System ◽  
Municipal Wastewater Treatment ◽  
Wastewater Treatment System ◽  
Pre Treatment ◽  
Organic Particles

This paper describes a pilot plant study on the performance of a hybrid small municipal wastewater treatment system consisting of a jet mixed separator(JMS) and upgraded RBC. The JMS was used as a pre-treatment of the RBC instead of the primary clarifier. The treatment capacity of the system was fixed at 100 m3/d, corresponding to the hydraulic loading to the RBC of 117 L/m2/d. The effluent from the grid chamber at a municipal wastewater treatment plant was fed into the hybrid system. The RBC was operated using the electric power produced by a solar electric generation panel with a surface area of 8 m2 under enough sunlight. In order to reduce the organic loading to the RBC, polyaluminium chloride(PAC) was added to the JMS influent to remove the colloidal and suspended organic particles. At the operational condition where the A1 dosage and hydraulic retention time of the JMS were fixed at 5 g/m3 and 45 min., respectively, the average effluent water quality of hybrid system was as follows: TOC=8 g/m3, Total BOD=8 g/m3, SS=8 g/m3, Turbidity=6 TU, NH4-N=7 g/m3, T-P=0.5 g/m3. In this operating condition, electric power consumption of the RBC for treating unit volume of wastewater is only 0.07 KWH/m3.

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