Application of Cell-free Expression Systems to Proteomic Studies

2003 ◽  
pp. 101-107 ◽  
Author(s):  
Peter T. Beernink ◽  
B. W. Segelke ◽  
M. A. Coleman
Keyword(s):  
Free Expression ◽  
Expression Systems

scholarly journals Local deuteration enables NMR observation of methyl groups in proteins from eukaryotic and cell‐free expression systems.

2021 ◽  
Author(s):  
Abhinav Dubey ◽  
Nikolay Stoyanov ◽  
Thibault Viennet ◽  
Sandeep Chhabra ◽  
Shantha Elter ◽  
...  
Keyword(s):  
Free Expression ◽  
Methyl Groups ◽  
Expression Systems

Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems

2014 ◽  
pp. 125-143 ◽  
Author(s):  
Lei Kai ◽  
Erika Orbán ◽  
Erik Henrich ◽  
Davide Proverbio ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Free Expression ◽  
Expression Systems

Protecting Linear DNA Templates in Cell-Free Expression Systems from Diverse Bacteria

2020 ◽  
Vol 9 (10) ◽  
pp. 2851-2855
Author(s):  
Sung Sun Yim ◽  
Nathan I. Johns ◽  
Vincent Noireaux ◽  
Harris H. Wang
Keyword(s):  
Free Expression ◽  
Expression Systems ◽  
Dna Templates ◽  
Linear Dna

scholarly journals Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems

2019 ◽  
Vol 2 (2) ◽  
pp. 39 ◽  
Author(s):  
Dohyun Jeong ◽  
Melissa Klocke ◽  
Siddharth Agarwal ◽  
Jeongwon Kim ◽  
Seungdo Choi ◽  
...  
Keyword(s):  
Synthetic Biology ◽  
Free Expression ◽  
Dna Nanostructures ◽  
Expression Systems ◽  
Natural Systems ◽  
The Past ◽  
Current Cell ◽  
Recent Developments ◽  
Biological Circuits

Synthetic biology integrates diverse engineering disciplines to create novel biological systems for biomedical and technological applications. The substantial growth of the synthetic biology field in the past decade is poised to transform biotechnology and medicine. To streamline design processes and facilitate debugging of complex synthetic circuits, cell-free synthetic biology approaches has reached broad research communities both in academia and industry. By recapitulating gene expression systems in vitro, cell-free expression systems offer flexibility to explore beyond the confines of living cells and allow networking of synthetic and natural systems. Here, we review the capabilities of the current cell-free platforms, focusing on nucleic acid-based molecular programs and circuit construction. We survey the recent developments including cell-free transcription–translation platforms, DNA nanostructures and circuits, and novel classes of riboregulators. The links to mathematical models and the prospects of cell-free synthetic biology platforms will also be discussed.

scholarly journals Metabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization

2019 ◽  
Vol 58 (50) ◽  
pp. 22472-22482 ◽  
Author(s):  
April M. Miguez ◽  
Monica P. McNerney ◽  
Mark P. Styczynski
Keyword(s):  
Escherichia Coli ◽  
Process Optimization ◽  
Metabolic Profiling ◽  
Free Expression ◽  
Expression Systems

scholarly journals Functional Expression of the PorAH Channel fromCorynebacterium glutamicumin Cell-free Expression Systems

2011 ◽  
Vol 286 (37) ◽  
pp. 32525-32532 ◽  
Author(s):  
Parthasarathi Rath ◽  
Pascal Demange ◽  
Olivier Saurel ◽  
Marielle Tropis ◽  
Mamadou Daffé ◽  
...  
Keyword(s):  
Functional Expression ◽  
Free Expression ◽  
Expression Systems

scholarly journals Preparation of amino acid mixtures for cell-free expression systems

BioTechniques ◽  
2015 ◽  
Vol 58 (1) ◽  
Author(s):  
Filippo Caschera ◽  
Vincent Noireaux
Keyword(s):  
Amino Acid ◽  
Free Expression ◽  
Expression Systems ◽  
Amino Acid Mixtures

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems

2012 ◽  
Vol 82 (2) ◽  
pp. 308-316 ◽  
Author(s):  
Stefan Haberstock ◽  
Christian Roos ◽  
Yvette Hoevels ◽  
Volker Dötsch ◽  
Gisela Schnapp ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Production ◽  
Systematic Approach ◽  
Free Expression ◽  
Expression Systems

scholarly journals A systematic approach for testing expression of human full-length proteins in cell-free expression systems

2007 ◽  
Vol 7 (1) ◽  
pp. 64 ◽  
Author(s):  
Claudia Langlais ◽  
Birgit Guilleaume ◽  
Nadja Wermke ◽  
Tina Scheuermann ◽  
Lars Ebert ◽  
...  
Keyword(s):  
Systematic Approach ◽  
Full Length ◽  
Free Expression ◽  
Expression Systems
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