Establishment of Cell Lines from Bombyx Mori amd B. Mandarina, and Attempt of Cell Fusion

1988 ◽  
pp. 243-246 ◽  
Author(s):  
O. Ninaki ◽  
K. Nagayasu ◽  
H. Kameoka ◽  
H. Fujiwara ◽  
N. Miyajima ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Cell Fusion ◽  
Cell Lines

Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori

2008 ◽  
Vol 133 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Jae Man Lee ◽  
Masateru Takahashi ◽  
Hiroaki Mon ◽  
Hitoshi Mitsunobu ◽  
Katsumi Koga ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Cell Lines ◽  
Insect Cell ◽  
Expression Systems ◽  
Insect Cell Lines ◽  
Silkworm Bombyx Mori

Quantitative cell fusion: the fusion sensitivity (FS) potential

1981 ◽  
Vol 49 (1) ◽  
pp. 87-97
Author(s):  
D. Rohme
Keyword(s):  
Cell Line ◽  
Cell Fusion ◽  
Cell Lines ◽  
Dose Response ◽  
Sendai Virus ◽  
Biological Significance ◽  
Diploid Cell ◽  
Mammalian Cell Lines ◽  
Virus Dose ◽  
A Cell

The dose response of Sendai virus-induced cell fusion was studied in 10 mammalian cell lines, comprising 5 continuous and 5 diploid cell lines originating from 5 species. The extent of fusion was calculated using a parameter directly proportional to the number of fusion events (t-parameter). At lower levels of fusion the dose response was found to be based on the same simple kinetic rules in all cell lines and was defined by the formula: t = FS. FAU/(I + FS. FAU), where FS (fusion sensitivity) is a cell-specific constant of the fusion rate and FAU (fusion activity units) is the virus dose. The FS potential of a cell line was determined as the linear regression coefficient of the fusion index (t/(I - t)) on the virus dose. At higher levels of fusion, when the fusion extent reached cell-line-specific maximal levels, the dose response was not as uniform. In general, and particularly in the cases of the diploid cell lines, these maximal levels were directly proportional to the FS potentials. Thus, it was concluded that the FS potential is the basic quantitative feature, which expresses the cellular fusion efficiency. The fact that FS varied extensively between cell lines, but at the same time apparently followed certain patterns (being higher in continuous compared to diploid cell lines and being related to the species of origin of the cells), emphasizes it biological significance as well as its possible usefulness in studies of the efficiency of various molecular interactions in the cell membrane/cytoskeleton system.

Gene Analysis by Blot Hybridization on the Silkworm, Bombyx Mori, Cell Lines

2018 ◽  
pp. 143-149
Author(s):  
O. Ninaki ◽  
N. Takada ◽  
H. Fujiwara ◽  
T. Ogura ◽  
N. Miyajima ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Cell Lines ◽  
Blot Hybridization ◽  
Gene Analysis ◽  
Silkworm Bombyx Mori

The Transcription Express Characteristics of Several Genes in the Process of Bombyx mori Ovarian Carcinoma

2013 ◽  
Vol 796 ◽  
pp. 39-42
Author(s):  
Xie Yi ◽  
Li Bing
Keyword(s):  
Ovarian Carcinoma ◽  
Gene Family ◽  
Bombyx Mori ◽  
Cell Lines ◽  
P53 Gene ◽  
Ovary Cell ◽  
Ovary Cells ◽  
Bmn Cell ◽  
Cyclin Gene ◽  
Materials Used

Bombyx mori cell line (BmN) comes fromBombyx moriovary cell subculture. In order to study the change of several genes transcription in the process ofBombyx moriovary cells primary culture and subculture, we usedBombyx moriovary organizations and BmN cell lines as research materials, used Real Time fluorescent quantitative RT-PCR to detect cyclin gene family (CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1), p53 and Telomerase genes transcription level in the ovary and BmN cell lines, and took Actin3 gene as reference to dispose the results. The results showed that in theBombyx moriBmN cell lines the expression of CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1 and Telomerase genes were higher than those in the ovary. The expression of CyclinB in the BmN was more then 3.8 which was 76 times higher than that in the ovary; The expression of p53 gene in the BmN cell was lower than that in the ovary; The expression of Telomerase gene in the BmN cell was higher than that in the ovary. The results accumulated a reliable data for further study on the the role of cyclin gene family, p53 gene, and Telomerase gene in the process ofBombyx moriovarian carcinoma.

scholarly journals Novel Bombyx mori cell lines cultivable at 37°C

1999 ◽  
Vol 34 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Shigeo Imanishi ◽  
Eun-Sook Cho ◽  
Shuichiro Tomita
Keyword(s):  
Bombyx Mori ◽  
Cell Lines

Heterologous promoter recognition leading to high-level expression of cloned foreign genes in Bombyx mori cell lines and larvae

Gene ◽  
1997 ◽  
Vol 190 (1) ◽  
pp. 181-189 ◽  
Author(s):  
Satyanarayana Sriram ◽  
Vikas B. Palhan ◽  
Karumathil P. Gopinathan
Keyword(s):  
Bombyx Mori ◽  
Cell Lines ◽  
High Level Expression ◽  
Heterologous Promoter ◽  
Promoter Recognition ◽  
Foreign Genes ◽  
High Level

New Reporter Cell Lines to Study Macrophage-Tropic HIV Envelope Protein-Mediated Cell-Cell Fusion

1999 ◽  
Vol 15 (18) ◽  
pp. 1667-1672 ◽  
Author(s):  
Yu-Long Hong ◽  
Lan-Hsin Wu ◽  
Mei Cui ◽  
Gary McMaster ◽  
Stephen W . Hunt ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Cell Lines ◽  
Envelope Protein ◽  
Reporter Cell ◽  
Hiv Envelope ◽  
Cell Cell
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