New Experimental Approaches to the Adoptive Immunotherapy of Cancer: Cytokines, Gene Therapy, Oncogenes and Transgenic Mice

1992 ◽  
pp. 165-188 ◽  
Author(s):  
B. A. Fox
Keyword(s):  
Gene Therapy ◽  
Transgenic Mice ◽  
Adoptive Immunotherapy ◽  
Immunotherapy Of Cancer ◽  
Experimental Approaches

Adoptive immunotherapy of cancer: biological response modifiers and cytotoxic cell therapy

Biotherapy ◽  
1992 ◽  
Vol 5 (2) ◽  
pp. 119-129 ◽  
Author(s):  
Gilda G. Hillman ◽  
Gabriel P. Haas ◽  
Werner H. Wahl ◽  
Denis M. Callewaert
Keyword(s):  
Cell Therapy ◽  
Adoptive Immunotherapy ◽  
Biological Response ◽  
Biological Response Modifiers ◽  
Cytotoxic Cell ◽  
Immunotherapy Of Cancer

T-Cell Adoptive Immunotherapy of Cancer

2006 ◽  
pp. 181-212
Author(s):  
Peter A. Cohen ◽  
Mohamed Awad ◽  
Suyu Shu
Keyword(s):  
T Cell ◽  
Adoptive Immunotherapy ◽  
Immunotherapy Of Cancer

Experimental Approaches to Hemophilia Gene Therapy: Gene Transfer into Hematopoietic Stem Cells

2004 ◽  
pp. 153-158
Author(s):  
A. Tiede ◽  
M. Eder ◽  
M. Scherr ◽  
A. Ganser ◽  
M. von Depka Prondzinski
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Gene Transfer ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Experimental Approaches

583 EVALUATION OF AAV-MEDIATED IFNa-GENE THERAPY EFFICACY IN HBV TRANSGENIC MICE: CONSTITUTIVE VERSUS INDUCIBLE EXPRESSION

2009 ◽  
Vol 50 ◽  
pp. S214-S215
Author(s):  
L. Vanrell ◽  
C. Olague ◽  
A. Vales ◽  
A. Pañeda ◽  
L. Tenembaum ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Transgenic Mice ◽  
Inducible Expression ◽  
Therapy Efficacy ◽  
Ifna Gene

Recent Advances in T Cell Adoptive Immunotherapy of Cancer

2008 ◽  
Vol 4 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Li Zhang ◽  
Pouneh Dokouhaki
Keyword(s):  
T Cell ◽  
Adoptive Immunotherapy ◽  
Recent Advances ◽  
Immunotherapy Of Cancer

Locus control region activity by 5′HS3 requires a functional interaction with β-globin gene regulatory elements: expression of novel β/γ-globin hybrid transgenes

Blood ◽  
2000 ◽  
Vol 95 (10) ◽  
pp. 3242-3249 ◽  
Author(s):  
Joel E. Rubin ◽  
Peter Pasceri ◽  
Xiumei Wu ◽  
Philippe Leboulch ◽  
James Ellis
Keyword(s):  
Gene Therapy ◽  
Transgenic Mice ◽  
Control Region ◽  
Globin Gene ◽  
Single Copy ◽  
Gene Sequences ◽  
Coding Sequences ◽  
Globin Promoter ◽  
Intron 2 ◽  
Chromatin Opening

Abstract The human β-globin locus control region (LCR) contains chromatin opening and transcriptional enhancement activities that are important to include in β-globin gene therapy vectors. We previously used single-copy transgenic mice to map chromatin opening activity to the 5′HS3 LCR element. Here, we test novel hybrid globin genes to identify β-globin gene sequences that functionally interact with 5′HS3. First, we show that an 850-base pair (bp) 5′HS3 element activates high-level β-globin gene expression in fetal livers of 17 of 17 transgenic mice, including 3 single-copy animals, but fails to reproducibly activate Aγ-globin transgenes. To identify the β-globin gene sequences required for LCR activity by 5′HS3, we linked the 815-bp β-globin promoter to Aγ-globin coding sequences (BGT34), together with either the β-globin intron 2 (BGT35), the β-globin 3′ enhancer (BGT54), or both intron 2 and the 3′ enhancer (BGT50). Of these transgenes, only BGT50 reproducibly expresses Aγ-globin RNA (including 7 of 7 single-copy animals, averaging 71% per copy). Modifications to BGT50 show that LCR activity is detected after replacing the β-globin promoter with the 700-bp Aγ-globin promoter, but is abrogated when an AT-rich region is deleted from β-globin intron 2. We conclude that LCR activity by 5′HS3 on globin promoters requires the simultaneous presence of β-globin intron 2 sequences and the 260-bp 3′ β-globin enhancer. The BGT50 construct extends the utility of the 5′HS3 element to include erythroid expression of nonadult β-globin coding sequences in transgenic animals and its ability to express antisickling γ-globin coding sequences at single copy are ideal characteristics for a gene therapy cassette.

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