Excision Repaired Sites, Chromosome Breaks and Chromosome Loss Measured Simultaneously in Human Lymphocytes using the Cytokinesis Block Micronucleus Assay and Cytosine Arabinoside

1994 ◽  
pp. 223-234
Author(s):  
M. Fenech
Keyword(s):  
Cytosine Arabinoside ◽  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Chromosome Loss ◽  
Chromosome Breaks

scholarly journals Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

2014 ◽  
Vol 67 (6) ◽  
pp. 1059-1066 ◽  
Author(s):  
Dilek Akyıl ◽  
Arzu Özkara ◽  
S. Feyza Erdoğmuş ◽  
Yasin Eren ◽  
Muhsin Konuk ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Micronucleus Assay

scholarly journals Doxorubicin-Induced Translocation of mtDNA into the Nuclear Genome of Human Lymphocytes Detected Using a Molecular-Cytogenetic Approach

2020 ◽  
Vol 21 (20) ◽  
pp. 7690
Author(s):  
Tigran Harutyunyan ◽  
Ahmed Al-Rikabi ◽  
Anzhela Sargsyan ◽  
Galina Hovhannisyan ◽  
Rouben Aroutiounian ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Nuclear Genome ◽  
Biological Phenomenon ◽  
Molecular Cytogenetic ◽  
Chromosome Breaks ◽  
Pathological Conditions ◽  
The Impact ◽  
In Vitro Treatment

Translocation of mtDNA in the nuclear genome is an ongoing process that contributes to the development of pathological conditions in humans. However, the causal factors of this biological phenomenon in human cells are poorly studied. Here we analyzed mtDNA insertions in the nuclear genome of human lymphocytes after in vitro treatment with doxorubicin (DOX) using a fluorescence in situ hybridization (FISH) technique. The number of mtDNA insertions positively correlated with the number of DOX-induced micronuclei, suggesting that DOX-induced chromosome breaks contribute to insertion events. Analysis of the odds ratios (OR) revealed that DOX at concentrations of 0.025 and 0.035 µg/mL significantly increases the rate of mtDNA insertions (OR: 3.53 (95% CI: 1.42–8.76, p < 0.05) and 3.02 (95% CI: 1.19–7.62, p < 0.05), respectively). Analysis of the distribution of mtDNA insertions in the genome revealed that DOX-induced mtDNA insertions are more frequent in larger chromosomes, which are more prone to the damaging action of DOX. Overall, our data suggest that DOX-induced chromosome damage can be a causal factor for insertions of mtDNA in the nuclear genome of human lymphocytes. It can be assumed that the impact of a large number of external and internal mutagenic factors contributes significantly to the origin and amount of mtDNA in nuclear genomes.

Determination of genotoxic effects of methidathion alkaline hydrolysis in human lymphocytes using the micronucleus assay and square-wave voltammetry

2017 ◽  
Vol 113 ◽  
pp. 9-14 ◽  
Author(s):  
Polychronis D. Stivaktakis ◽  
Evangelos Giannakopoulos ◽  
Dimitris Vlastos ◽  
Demetrios P. Matthopoulos
Keyword(s):  
Alkaline Hydrolysis ◽  
Square Wave Voltammetry ◽  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Genotoxic Effects ◽  
Square Wave ◽  
Wave Voltammetry

QUALITATIVE EFFECT OF CYTOSINE ARABINOSIDE UPON TRANSCRIPTION IN CULTURED HUMAN LYMPHOCYTES

1972 ◽  
Vol 5 (6) ◽  
pp. 535-537
Author(s):  
R. A. Flickinger ◽  
F. M. Roche ◽  
L. V. Rodriguez
Keyword(s):  
Cytosine Arabinoside ◽  
Human Lymphocytes ◽  
Qualitative Effect

scholarly journals Genotoxicity induced by arsenic compounds in peripheral human lymphocytes analysed by cytokinesis-block micronucleus assay

Mutagenesis ◽  
2007 ◽  
Vol 22 (4) ◽  
pp. 255-261 ◽  
Author(s):  
R. Colognato ◽  
F. Coppede ◽  
J. Ponti ◽  
E. Sabbioni ◽  
L. Migliore
Keyword(s):  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Arsenic Compounds

The micronucleus assay in human lymphocytes: screening for inter-individual variability and application to biomonitoring

1994 ◽  
Vol 15 (2) ◽  
pp. 313-317 ◽  
Author(s):  
C.Di Giorgio ◽  
M.P. De Méo ◽  
M. Laget ◽  
H. Guiraud ◽  
A. Botta ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Individual Variability
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