Differentiation in the Cellular Slime Mold

1968 ◽  
pp. 115-129
Author(s):  
Barbara E. Wright
Keyword(s):  
Slime Mold ◽  
Cellular Slime Mold ◽  
Cellular Slime

Acidic ribosomal proteins of Dictyostelium discoideum that show electrophoretic similarity to Escherichia coli proteins L7 and L12

1989 ◽  
Vol 67 (10) ◽  
pp. 712-718 ◽  
Author(s):  
S. Ramagopal
Keyword(s):  
Escherichia Coli ◽  
Dictyostelium Discoideum ◽  
Gel Electrophoresis ◽  
Ribosomal Proteins ◽  
Sodium Dodecyl ◽  
Slime Mold ◽  
Cellular Slime Mold ◽  
Two Dimensional ◽  
Cellular Slime ◽  
Acidic Proteins

This study documents the presence of three acidic proteins, A1 (pI 4.95), A2 (pI 4.85), and A3 (pI 4.70), in Dictyostelium discoideum ribosomes. All three proteins showed an apparent molecular mass of 13 000 by two-dimensional, sodium dodecyl sulfate gel electrophoresis. They were selectively released by treatment of ribosomes with 50% ethanol – 1 M NH4Cl. The amino acid compositions of A1, A2, and A3 were identical and indicated a predominant amount of alanine. All the above properties are shared by Escherichia coli proteins L7 and L12 and acidic ribosomal proteins in many eukaryotes. Unlike other eukaryotic systems, the acidic proteins of D. discoideum were found associated with the 40S rather than the 60S ribosomal subunit. Acidic proteins analogous in size and electrophoretic mobility to those of D. discoideum were also detected in several other cellular slime mold strains. Not one of the cellular slime mold acidic proteins reacted with antibodies to E. coli proteins L7 and L12 in immunodiffusion tests. In D. discoideum, the distribution of acidic proteins was altered during development. Amoebae contained all three proteins. In spores, A, was absent and the relative amounts of A2 and A3 were lower than in amoebae. In addition, nine other acidic ribosomal proteins exhibited differences between vegetative amoebae and spores.Key words: acidic ribosomal proteins, development, cellular slime mold, L7 and L12 proteins, two-dimensional gel electrophoresis.

The stabilization of morphological field size during slime mold morphogenesis

Development ◽  
1978 ◽  
Vol 44 (1) ◽  
pp. 45-51
Author(s):  
Michael Peacock ◽  
David R. Soll
Keyword(s):  
Dictyostelium Discoideum ◽  
Aggregate Size ◽  
Slime Mold ◽  
Field Size ◽  
Cellular Slime Mold ◽  
Slime Mold Dictyostelium Discoideum ◽  
Cellular Slime ◽  
The Relationship ◽  
Tip Formation

The relationship between aggregate size and morphological field size has been investigated in the cellular slime mold Dictyostelium discoideum. Evidence is presented that aggregate size and field size exhibit different temperature sensitivities and that an aggregate can be induced to separate into several morphological fields by a decrease in temperature. In addition, evidence is presented that field size is stabilized at a point in time just prior to tip formation.

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