Methylene Blue Stain of the Urinary Sediment

1977 ◽  
pp. 49-50
Author(s):  
Herman J. de Voogt ◽  
Peter Rathert ◽  
Mathilde E. Beyer-Boon
Keyword(s):  
Methylene Blue ◽  
Urinary Sediment ◽  
Blue Stain

A COMPARISON OF SIX METHODS OF PREPARING AND USING THE METHYLENE BLUE STAIN FOR BACTERIAL COUNTS BY THE DIRECT MICROSCOPIC METHOD*

1954 ◽  
Vol 17 (6) ◽  
pp. 179-184 ◽  
Author(s):  
Nathan Mantel ◽  
A. H. Robertson
Keyword(s):  
Methylene Blue ◽  
Federal State ◽  
Cooperative Study ◽  
Microscopic Method ◽  
Bacterial Counts ◽  
Blue Stain ◽  
Microscopic Count

A cooperative study, involving 12 federal, state, local, and private laboratories throughout the country, was conducted to evaluate six methods of preparing and using the methylene blue stain for the direct microscopic count of bacteria in milk. Three of the six methods were found superior, yielding significantly higher bacteria counts, at the same time providing greater ease in counting. These three methods are: Levine and Black's acid-and-water-free stain; North's aniline oil stain; and Anderson's polychrome stain.

scholarly journals PHENOL METHYLENE BLUE STAIN

1926 ◽  
Vol 16 (10) ◽  
pp. 1049-1049
Author(s):  
Theodore C. Buck
Keyword(s):  
Methylene Blue ◽  
Blue Stain

EFFECT OF PASTEURIZATION ON THE DIRECT MICROSCOPIC COUNT OF EGGS

1971 ◽  
Vol 34 (4) ◽  
pp. 209-211 ◽  
Author(s):  
H. E. Hall ◽  
D. F. Brown ◽  
R. B. Read
Keyword(s):  
Methylene Blue ◽  
Bacterial Count ◽  
Bacterial Cells ◽  
Bacterial Counts ◽  
The North ◽  
Counting Chamber ◽  
Blue Stain ◽  
Microscopic Count ◽  
Whole Egg

When pasteurized whole eggs from breakers were examined by the Direct Microscopic Count (DMC) procedure, the bacterial count frequently appeared to be too low to correlate with the observed state of decomposition. The DMC of whole egg was found to decrease during pasteurization. To determine why, DMC's were done using the North Aniline Oil - Methylene Blue Stain and the Levowitz-Weber modification of the Newman-Lampert stain. Total bacterial counts also were made using the Petroff-Hausser counting chamber. Results indicated that the reduction in count resulted from lysis of some of the bacterial cells in egg rather than to loss of stainability. Crystalline lysozyme at the concentration found in egg and whole egg preparations produced similar reductions in the DMC of bacteria isolated from egg.

scholarly journals Fungal keratitis: Rapid diagnosis using methylene blue stain

2015 ◽  
Vol 2 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Dalia Moemen ◽  
Tamer Bedir ◽  
Eman A. Awad ◽  
Adel Ellayeh
Keyword(s):  
Methylene Blue ◽  
Rapid Diagnosis ◽  
Fungal Keratitis ◽  
Blue Stain

The choice of chromogen and reliability of contact rhinoscopy in the irradiated nasopharynx

2007 ◽  
Vol 122 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Martin W Pak ◽  
Samuel Chow ◽  
C A van Hasselt
Keyword(s):  
Methylene Blue ◽  
Squamous Metaplasia ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Cross Sectional ◽  
Vital Stain ◽  
Intraclass Correlation Coefficients ◽  
Observer Reliability ◽  
Blue Stain ◽  
The Mean

AbstractA cross-sectional randomised single blind study was conducted to assess how concentrations of chromogen (vital stain) and the characteristics of the assessors affect the assessment of contact rhinoscopy. Twenty-eight patients who had undergone external radiotherapy for nasopharyngeal carcinoma were assessed by contact rhinoscopy using 0.5 per cent and 1 per cent methylene blue stain on opposite sides of the nasopharynx. Three independent observers assessed the visual clarity of the 45 contact endoscopic images showing squamous metaplasia according to a visual analogue scale. The intraclass correlation coefficients were 0.916 to 0.957 and 0.839 to 0.964 for intra-observer reliability of assessors in the groups of 0.5 per cent and 1 per cent stains, respectively. The intraclass correlation coefficients for inter-observer reliability of assessors were 0.884 and 0.885 in the groups of 0.5 per cent and 1 per cent stains, respectively. The mean scores of clarity of the cellular details were statistically higher in the group of 1 per cent stain among all assessors. These results showed that the assessment of squamous metaplasia by contact endoscopy is highly reliable irrespective of the clinical experience and knowledge of histopathology of the assessors. One per cent methylene blue should be the vital stain of choice in contact endoscopy.

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