Note Regarding the Methylene Blue Stain for Anthrax Blood

1922 ◽  
Vol 35 ◽  
pp. 232-233
Author(s):  
John M'Fadyean
Keyword(s):  
Methylene Blue ◽  
Blue Stain

A COMPARISON OF SIX METHODS OF PREPARING AND USING THE METHYLENE BLUE STAIN FOR BACTERIAL COUNTS BY THE DIRECT MICROSCOPIC METHOD*

1954 ◽  
Vol 17 (6) ◽  
pp. 179-184 ◽  
Author(s):  
Nathan Mantel ◽  
A. H. Robertson
Keyword(s):  
Methylene Blue ◽  
Federal State ◽  
Cooperative Study ◽  
Microscopic Method ◽  
Bacterial Counts ◽  
Blue Stain ◽  
Microscopic Count

A cooperative study, involving 12 federal, state, local, and private laboratories throughout the country, was conducted to evaluate six methods of preparing and using the methylene blue stain for the direct microscopic count of bacteria in milk. Three of the six methods were found superior, yielding significantly higher bacteria counts, at the same time providing greater ease in counting. These three methods are: Levine and Black's acid-and-water-free stain; North's aniline oil stain; and Anderson's polychrome stain.

scholarly journals PHENOL METHYLENE BLUE STAIN

1926 ◽  
Vol 16 (10) ◽  
pp. 1049-1049
Author(s):  
Theodore C. Buck
Keyword(s):  
Methylene Blue ◽  
Blue Stain

EFFECT OF PASTEURIZATION ON THE DIRECT MICROSCOPIC COUNT OF EGGS

1971 ◽  
Vol 34 (4) ◽  
pp. 209-211 ◽  
Author(s):  
H. E. Hall ◽  
D. F. Brown ◽  
R. B. Read
Keyword(s):  
Methylene Blue ◽  
Bacterial Count ◽  
Bacterial Cells ◽  
Bacterial Counts ◽  
The North ◽  
Counting Chamber ◽  
Blue Stain ◽  
Microscopic Count ◽  
Whole Egg

When pasteurized whole eggs from breakers were examined by the Direct Microscopic Count (DMC) procedure, the bacterial count frequently appeared to be too low to correlate with the observed state of decomposition. The DMC of whole egg was found to decrease during pasteurization. To determine why, DMC's were done using the North Aniline Oil - Methylene Blue Stain and the Levowitz-Weber modification of the Newman-Lampert stain. Total bacterial counts also were made using the Petroff-Hausser counting chamber. Results indicated that the reduction in count resulted from lysis of some of the bacterial cells in egg rather than to loss of stainability. Crystalline lysozyme at the concentration found in egg and whole egg preparations produced similar reductions in the DMC of bacteria isolated from egg.

scholarly journals Fungal keratitis: Rapid diagnosis using methylene blue stain

2015 ◽  
Vol 2 (4) ◽  
pp. 289-294 ◽  
Author(s):  
Dalia Moemen ◽  
Tamer Bedir ◽  
Eman A. Awad ◽  
Adel Ellayeh
Keyword(s):  
Methylene Blue ◽  
Rapid Diagnosis ◽  
Fungal Keratitis ◽  
Blue Stain

The choice of chromogen and reliability of contact rhinoscopy in the irradiated nasopharynx

2007 ◽  
Vol 122 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Martin W Pak ◽  
Samuel Chow ◽  
C A van Hasselt
Keyword(s):  
Methylene Blue ◽  
Squamous Metaplasia ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Cross Sectional ◽  
Vital Stain ◽  
Intraclass Correlation Coefficients ◽  
Observer Reliability ◽  
Blue Stain ◽  
The Mean

AbstractA cross-sectional randomised single blind study was conducted to assess how concentrations of chromogen (vital stain) and the characteristics of the assessors affect the assessment of contact rhinoscopy. Twenty-eight patients who had undergone external radiotherapy for nasopharyngeal carcinoma were assessed by contact rhinoscopy using 0.5 per cent and 1 per cent methylene blue stain on opposite sides of the nasopharynx. Three independent observers assessed the visual clarity of the 45 contact endoscopic images showing squamous metaplasia according to a visual analogue scale. The intraclass correlation coefficients were 0.916 to 0.957 and 0.839 to 0.964 for intra-observer reliability of assessors in the groups of 0.5 per cent and 1 per cent stains, respectively. The intraclass correlation coefficients for inter-observer reliability of assessors were 0.884 and 0.885 in the groups of 0.5 per cent and 1 per cent stains, respectively. The mean scores of clarity of the cellular details were statistically higher in the group of 1 per cent stain among all assessors. These results showed that the assessment of squamous metaplasia by contact endoscopy is highly reliable irrespective of the clinical experience and knowledge of histopathology of the assessors. One per cent methylene blue should be the vital stain of choice in contact endoscopy.

Contact Endoscopy for Intraoperative Parathyroid Identification

2003 ◽  
Vol 112 (3) ◽  
pp. 242-245 ◽  
Author(s):  
Rogério A. Dedivitis ◽  
André V. Guimarães
Keyword(s):  
Methylene Blue ◽  
Adipose Tissue ◽  
Thyroid Surgery ◽  
Parathyroid Tissue ◽  
Parathyroid Glands ◽  
The Other ◽  
Before And After ◽  
Blue Stain ◽  
Thyroid Parenchyma

Experienced thyroid surgeons are often able to identify the parathyroid glands, but sometimes it is difficult to differentiate them from other contiguous tissues. Contact endoscopy was introduced in otolaryngology for the characterization of normal and pathological epithelia. Our objective was to analyze contact endoscopy as an auxiliary method for identification of the parathyroid glands during thyroid surgery. Five total thyroidectomies and 5 hemithyroidectomies were performed in September 2001. After surgical exposure, contact endoscopy was performed. A total of 15 peritracheal regions were studied. Superior and inferior parathyroid tissues were identified on the basis of color, size, and probable location. Contact endoscopy was performed before and after use of methylene blue stain. Contact endoscopy was also used in neighboring areas. We compared the visual impression to the contact endoscopy findings. Two structures were visually supposed to be the superior and inferior parathyroid glands in each case. From 30 visually supposed glands, 25 were confirmed by telescope. Of the other 5 structures initially supposed to be parathyroid tissue, 3 were adipose tissue and 2 were thyroid parenchyma. In the 5 cases in which the identification of one of the glands was not confirmed, an additional contact examination enabled us to further identify parathyroid glands in 3 cases in which structures were initially identified as adipose tissue. Contact endoscopy is an efficient auxiliary method for the identification of the parathyroid glands during thyroid surgery that poses little risk of morbidity to the patient.

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