How to Understand Estrogen Signaling from the Phenotypes of ERα and ERβ Knockout Mice

2004 ◽  
pp. 63-77
Author(s):  
M. Warner ◽  
J.-Å. Gustafsson
Keyword(s):  
Knockout Mice ◽  
Estrogen Signaling

scholarly journals The Novel Estrogen Receptor, G Protein-Coupled Receptor 30, Mediates the Proliferative Effects Induced by 17β-Estradiol on Mouse Spermatogonial GC-1 Cell Line

Endocrinology ◽  
2008 ◽  
Vol 149 (10) ◽  
pp. 5043-5051 ◽  
Author(s):  
Rosa Sirianni ◽  
Adele Chimento ◽  
Carmen Ruggiero ◽  
Arianna De Luca ◽  
Rosamaria Lappano ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathways ◽  
Cell Line ◽  
G Protein ◽  
Knockout Mice ◽  
Growth Factor Receptor ◽  
Estrogen Signaling ◽  
G Protein Coupled Receptor ◽  
17Β Estradiol ◽  
G Protein Coupled

Many studies have indicated that estrogens could have a role in the regulation of testicular function. However, it remains uncertain whether estrogens are able to directly activate signaling pathways in male germ cells. Estrogens are synthesized by the enzyme aromatase and classically act by binding to estrogen receptors (ERs)-α and ERβ. Knockout mice for both receptor isoforms exhibit a testicular phenotype that is less severe than aromatase knockout mice, suggesting the existence of an estrogen-binding receptor that may compensate for the lack of ERs. Recently studies using estrogen-sensitive tumor cell lines have demonstrated that the G-protein-coupled receptor (GPR)-30 binds and mediates estrogen action through the activation of the epidermal growth factor receptor (EGFR)/ERK/fos transduction pathway. The present study investigated the ability of 17β-estradiol (E2) to activate this pathway in the mouse spermatogonial cell line (GC-1). Using the GC-1 cell line as a model system, we demonstrated that GC-1 cells express GPR30 and ERα but not ERβ. E2, the selective GPR30 agonist G1, and the selective ERα agonist 4,4′,4″-(4-propyl-[1H]pyrazole-1,3,5-triyl) trisphenol activated the rapid ERK1/2-fos signaling cascade. This response was abrogated by the EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI 182780, or by silencing GPR30 expression. Moreover, E2 and G1 up-regulated cyclin D1 expression and GC-1 cell proliferation. Our results indicate for the first time that estrogens, through a cross talk between GPR30 and ERα, activate the rapid EGFR/ERK/fos pathway, which in turn stimulate mouse GC-1 cell proliferation. Further studies to elucidate the involvement of rapid estrogen signaling pathways in the regulation of male fertility are warranted.

Heat stable toxin of E. coli (STa) stimulates duodenal mucosal bicarbonate secretion in cystic fibrosis knockout mice

2001 ◽  
Vol 120 (5) ◽  
pp. A137-A137
Author(s):  
D CHILDS ◽  
D CROMBIE ◽  
V PRATHA ◽  
Z SELLERS ◽  
D HOGAN ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Knockout Mice ◽  
Bicarbonate Secretion ◽  
E Coli ◽  
Heat Stable

Sa1495 LEPTIN AUGMENTS BILIARY HYPERPLASIA AND HEPATIC FIBROSIS IN MDR2-KNOCKOUT MICE

2020 ◽  
Vol 158 (6) ◽  
pp. S-1310
Author(s):  
Rebekah John ◽  
Anca D. Petrescu ◽  
Stephanie Grant ◽  
Elaina Williams ◽  
Sharon DeMorrow
Keyword(s):  
Hepatic Fibrosis ◽  
Knockout Mice

Abstract #219: Evidence of Insulitis in Signal Transducer and Activator of Transcription 5 (Stat5) Knockout Mice

2017 ◽  
Vol 23 ◽  
pp. 39
Author(s):  
Aili Guo ◽  
Nigel Daniels ◽  
Craig Nunemaker ◽  
Samantha J. Shaw ◽  
Karen Coschigano
Keyword(s):  
Knockout Mice ◽  
Signal Transducer

Xpa and Xpa/p53 +/- Knockout Mice: Overview of Available Data

2001 ◽  
Vol 29 (5) ◽  
pp. 117-127 ◽  
Author(s):  
Coen F. van Kreijl ◽  
Peter A. McAnulty ◽  
Rudolf B. Beems ◽  
An Vynckier ◽  
Harry van Steeg ◽  
...  
Keyword(s):  
Knockout Mice

Differential expression of PPARalpha, STAT1 and STAT3 in TAA/EtOH-induced liver fibrosis in cannabinoid recepetor 1 knockout mice

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
K Quint ◽  
M Stoll ◽  
T Wissniowski ◽  
A Agaimy ◽  
F Stickel ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Differential Expression ◽  
Knockout Mice

Susceptibility of LC3B Knockout Mice to Lung Injury and Fibrosis

2019 ◽  
Author(s):  
Vidya Kesireddy ◽  
Saket Ahuja ◽  
Shashi Chillappagari ◽  
Lars Knudsen ◽  
Ingrid Henneke ◽  
...  
Keyword(s):  
Lung Injury ◽  
Knockout Mice
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