Molecular Mapping of Antigenic and Cytotoxic T Lymphocyte Recognition Determinants on the Major Histocompatibility Complex Class I Molecule Kb Using In Vitro Mutant Cell Lines

1989 ◽  
pp. 130-131
Author(s):  
Parthasarathi Ajitkumar ◽  
Steven Geier ◽  
Francescopaolo Borriello ◽  
Mayuma Nakagawa ◽  
Jeffrey Bluestone ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Cell Lines ◽  
T Lymphocyte ◽  
Molecular Mapping ◽  
Cytotoxic T Lymphocyte ◽  
Mutant Cell ◽  
Complex Class ◽  
Histocompatibility Complex

scholarly journals The Cysteine-Rich Region and Secreted Form of the Attachment G Glycoprotein of Respiratory Syncytial Virus Enhance the Cytotoxic T-Lymphocyte Response despite Lacking Major Histocompatibility Complex Class I-Restricted Epitopes

2006 ◽  
Vol 80 (12) ◽  
pp. 5854-5861 ◽  
Author(s):  
Alexander Bukreyev ◽  
Maria Elina Serra ◽  
Federico R. Laham ◽  
Guillermina A. Melendi ◽  
Steven R. Kleeberger ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Respiratory Syncytial Virus ◽  
Major Histocompatibility Complex Class ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Complex Class ◽  
Rich Region ◽  
Ctl Response ◽  
Histocompatibility Complex ◽  
Syncytial Virus

ABSTRACT The cytotoxic T-lymphocyte (CTL) response is important for the control of viral replication during respiratory syncytial virus (RSV) infection. The attachment glycoprotein (G) of RSV does not encode major histocompatibility complex class I-restricted epitopes in BALB/c mice (H-2 d ). Furthermore, studies to date have described an absence of significant CTL activity directed against this protein in humans. Therefore, G previously was not considered necessary for the generation of RSV-specific CTL responses. In this study, we demonstrate that, despite lacking H-2d-restricted epitopes, G enhances the generation of an effective CTL response against RSV. Furthermore, we show that this stimulatory effect is independent of virus titers and RSV-induced inflammation; that it is associated primarily with the secreted form of G; and that the effect depends on the cysteine-rich region of G (GCRR), a segment conserved in wild-type isolates worldwide. These findings reveal a novel function for the GCRR with potential implications for the generation of protective cellular responses and vaccine development.

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