Stereotaxic intrastriatal implantation of human retinal pigment epithelial (hRPE) cells attached to gelatin microcarriers: a potential new cell therapy for Parkinson’s disease

2003 ◽  
pp. 215-227 ◽  
Author(s):  
R. L. Watts ◽  
C. D. Raiser ◽  
N. P. Stover ◽  
M. L. Cornfeldt ◽  
A. W. Schweikert ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cell Therapy ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Pigment Epithelial

scholarly journals PEDF and VEGF-A Output from Human Retinal Pigment Epithelial Cells Grown on Novel Microcarriers

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Torsten Falk ◽  
Nicole R. Congrove ◽  
Shiling Zhang ◽  
Alexander D. McCourt ◽  
Scott J. Sherman ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Clinical Trials ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Term Survival ◽  
Pigment Epithelial ◽  
Cell Based Therapy

Human retinal pigment epithelial (hRPE) cells have been tested as a cell-based therapy for Parkinson’s disease but will require additional study before further clinical trials can be planned. We now show that the long-term survival and neurotrophic potential of hRPE cells can be enhanced by the use of FDA-approved plastic-based microcarriers compared to a gelatin-based microcarrier as used in failed clinical trials. The hRPE cells grown on these plastic-based microcarriers display several important characteristics of hRPE foundin vivo: (1) characteristic morphological features, (2) accumulation of melanin pigment, and (3) high levels of production of the neurotrophic factors pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor-A (VEGF-A). Growth of hRPE cells on plastic-based microcarriers led to sustained levels (>1 ng/ml) of PEDF and VEGF-A in conditioned media for two months. We also show that the expression of VEGF-A and PEDF is reciprocally regulated by activation of the GPR143 pathway. GPR143 is activated by L-DOPA (1 μM) which decreased VEGF-A secretion as opposed to the previously reported increase in PEDF secretion. The hRPE microcarriers are therefore novel candidate delivery systems for achieving long-term delivery of the neuroprotective factors PEDF and VEGF-A, which could have a value in neurodegenerative conditions such as Parkinson’s disease.

scholarly journals Selective Large-Area Retinal Pigment Epithelial Removal by Microsecond Laser in Preparation for Cell Therapy

2021 ◽  
Vol 10 (10) ◽  
pp. 17
Author(s):  
Christian Burri ◽  
Sami Al-Nawaiseh ◽  
Philip Wakili ◽  
Simon Salzmann ◽  
Christina Krötz ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Large Area ◽  
Pigment Epithelial

Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy

Biomaterials ◽  
2008 ◽  
Vol 29 (7) ◽  
pp. 869-876 ◽  
Author(s):  
J WIKSTROM ◽  
M ELOMAA ◽  
H SYVAJARVI ◽  
J KUOKKANEN ◽  
M YLIPERTTULA ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Therapy ◽  
Cell Line ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Epithelial Cell Line ◽  
Pigment Epithelial
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