Integrated Vascular Engineering: Vascularization of Reconstructed Tissue

2016 ◽  
pp. 297-332 ◽  
Author(s):  
Ryo Sudo ◽  
Seok Chung ◽  
Yoojin Shin ◽  
Kazuo Tanishita
Keyword(s):  
Vascular Engineering

Cross-Linked Poly(trimethylene carbonate-co-l-lactide) as a Biodegradable, Elastomeric Scaffold for Vascular Engineering Applications

2011 ◽  
Vol 12 (11) ◽  
pp. 3856-3869 ◽  
Author(s):  
Bronwin L. Dargaville ◽  
Cédryck Vaquette ◽  
Hui Peng ◽  
Firas Rasoul ◽  
Yu Qian Chau ◽  
...  
Keyword(s):  
Trimethylene Carbonate ◽  
Engineering Applications ◽  
Vascular Engineering

Collagen-Silk Fibroin Fibers: A Promising Scaffold for Vascular Tissue Engineering

2012 ◽  
Vol 706-709 ◽  
pp. 572-577
Author(s):  
Estelle Paternotte ◽  
Mariana Agostini de Moraes ◽  
Marisa Masumi Beppu ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Research Teams ◽  
Cellular Attachment ◽  
New Models ◽  
Vascular Engineering ◽  
Vascular Replacement

Small caliber vascular replacement (<4 mm) still remains a challenge for medical and research teams, as no available vascular substitutes (VS) are suitable for small diameter bypass. Vascular engineering proposes new models of small diameter VS but rare are those that meet the biocompatibility and mechanical criteria. In this study, we developed a new scaffold made by the combination of two natural biomacromolecules: collagen and silk fibroin. The scaffold was further cellularised with porcine smooth muscle cells. First, the behavior of cells in the collagen-fibroin constructs was verified in order to evaluate the biocompatibility of the scaffold with the cells. Then, gel mass loss and cellular attachment, morphology, spreading and viability were analysed. The results showed an excellent interaction and biocompatibility between collagen, silk fibroin fibers and cells. Thus, the collagen-fibroin construct appears to be a very attractive material for vascular tissue engineering.

Stem cells for vascular engineering

2015 ◽  
pp. 621-639
Author(s):  
Y. Eugene Chen ◽  
Changqing Xie ◽  
Bo Yang
Keyword(s):  
Stem Cells ◽  
Vascular Engineering

Human-derived extracellular matrix from Wharton’s jelly: An untapped substrate to build up a standardized and homogeneous coating for vascular engineering

2017 ◽  
Vol 48 ◽  
pp. 227-237 ◽  
Author(s):  
Pan Dan ◽  
Émilie Velot ◽  
Grégory Francius ◽  
Patrick Menu ◽  
Véronique Decot
Keyword(s):  
Extracellular Matrix ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Vascular Engineering

scholarly journals Umbilical Cord Pericytes Provide a Viable Alternative to Mesenchymal Stem Cells for Neonatal Vascular Engineering

2021 ◽  
Vol 7 ◽  
Author(s):  
William Cathery ◽  
Ashton Faulkner ◽  
Eva Jover ◽  
Iker Rodriguez-Arabaolaza ◽  
Anita C. Thomas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Umbilical Cord ◽  
Somatic Growth ◽  
Tube Formation ◽  
Endothelial Cell Migration ◽  
Cell Monolayers ◽  
Vascular Engineering ◽  
Antigenic Phenotype

Reconstructive surgery of congenital heart disease (CHD) remains inadequate due to the inability of prosthetic grafts to match the somatic growth of pediatric patients. Functionalization of grafts with mesenchymal stem cells (MSCs) may provide a solution. However, MSCs represent a heterogeneous population characterized by wide diversity across different tissue sources. Here we investigated the suitability of umbilical cord pericytes (UCPs) in neonatal vascular engineering. Explant outgrowth followed by immunomagnetic sorting was used to isolate neural/glial antigen 2 (NG2)+/CD31− UCPs. Expanded NG2 UCPs showed consistent antigenic phenotype, including expression of mesenchymal and stemness markers, and high proliferation rate. They could be induced to a vascular smooth muscle cell-like phenotype after exposure to differentiation medium, as evidenced by the expression of transgelin and smooth muscle myosin heavy chain. Analysis of cell monolayers and conditioned medium revealed production of extracellular matrix proteins and the secretion of major angiocrine factors, which conferred UCPs with ability to promote endothelial cell migration and tube formation. Decellularized swine-derived grafts were functionalized using UCPs and cultured under static and dynamic flow conditions. UCPs were observed to integrate into the outer layer of the graft and modify the extracellular environment, resulting in improved elasticity and rupture strain in comparison with acellular grafts. These findings demonstrate that a homogeneous pericyte-like population can be efficiently isolated and expanded from human cords and integrated in acellular grafts currently used for repair of CHD. Functional assays suggest that NG2 UCPs may represent a viable option for neonatal tissue engineering applications.

Vascular Engineering to Make Blood-Compatible Surface

2016 ◽  
pp. 221-230
Author(s):  
Hiroshi Ujiie ◽  
Yoshiaki Suzuki ◽  
Dieter Liepsch
Keyword(s):  
Vascular Engineering

Biomaterials Approaches in Vascular Engineering: a Review of Past and Future Trends

2011 ◽  
pp. 457-487 ◽  
Author(s):  
Donny Hanjaya-Putra ◽  
Maureen Wanjare ◽  
Sharon Gerecht
Keyword(s):  
Future Trends ◽  
Vascular Engineering
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