In Vitro Conservation of Plant Germplasm

2015 ◽  
pp. 417-443 ◽  
Author(s):  
P. E. Rajasekharan ◽  
Leela Sahijram
Keyword(s):  
In Vitro Conservation ◽  
Plant Germplasm

In vitro conservation of tropical plant germplasm ? a review

Euphytica ◽  
1991 ◽  
Vol 57 (3) ◽  
pp. 227-243 ◽  
Author(s):  
F. Engelmann
Keyword(s):  
In Vitro Conservation ◽  
Plant Germplasm ◽  
Tropical Plant

scholarly journals In vitro Conservation and Cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub

2013 ◽  
Vol 41 (2) ◽  
pp. 638 ◽  
Author(s):  
Aylin OZUDOGRU ◽  
Diogo Pedrosa Corrêa Da SILVA ◽  
Ergun KAYA ◽  
Giuliano DRADI ◽  
Renato PAIVA ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Storage Temperature ◽  
Sucrose Concentration ◽  
Aluminum Foil ◽  
Shoot Tips ◽  
In Vitro Conservation ◽  
Shoot Cultures ◽  
Storage Medium ◽  
Droplet Vitrification

The study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.

IN VITRO CONSERVATION AND CRYOPRESERVATION IN NATIONAL GENEBANK OF CHINA

2014 ◽  
pp. 309-317 ◽  
Author(s):  
Jinmei Zhang ◽  
Xia Xin ◽  
Guangkun Yin ◽  
Xinxiong Lu ◽  
Xiaoling Chen
Keyword(s):  
In Vitro Conservation

scholarly journals In vitro conservation of taro (Colocasia esculenta Var. Globulifera) as influenced by mannitol

2016 ◽  
Vol 41 (1) ◽  
pp. 67-74
Author(s):  
MKR Bhuiyan ◽  
MJ Hossain ◽  
MM Haque
Keyword(s):  
Plant Height ◽  
Vital Role ◽  
Colocasia Esculenta ◽  
Experimental Results ◽  
In Vitro Conservation ◽  
Axillary Bud ◽  
Conventional System ◽  
Maintenance Breeding

In vitro conservation of germplasm plays a vital role in maintenance breeding and also has many advantages over the conventional system. The experimental results for conservation of Colocasia sp. also proved this. In relation to explants and osmoticum, meristem and axillary bud could be conserved for 24 months while meristem-base died after 6 months. Mannitol as osmoticum @ 4% performed nicely to conserve Colocasia upto 24 months. Only meristem and axillary bud could be conserved for 24 months with the use of 4 % mannitol. But other level of mannitol remained culture alive for varying periods (6 to 12 months). After 24 months, the plant height was 6.5 cm for the meristem and 6.4 for axillary bud.Bangladesh J. Agril. Res. 41(1): 67-74, March 2016

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