Experimental Procedures to Assay Invasion-Associated Activities of Primary Cultured Fibroblasts

2011 ◽  
pp. 131-143
Author(s):  
An Hendrix ◽  
Koen Jacobs ◽  
Astrid De Boeck ◽  
Wendy Westbroek ◽  
Marc Bracke ◽  
...  
Keyword(s):  
Cultured Fibroblasts

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

scholarly journals Synthesis and release of procollagenase by cultured fibroblasts.

1976 ◽  
Vol 251 (10) ◽  
pp. 3162-3168 ◽  
Author(s):  
H Birkedal-Hansen ◽  
C M Cobb ◽  
R E Taylor ◽  
H M Fullmer
Keyword(s):  
Cultured Fibroblasts

A PEX10 defect in a patient with no detectable defect in peroxisome assembly or metabolism in cultured fibroblasts

2008 ◽  
Vol 32 (1) ◽  
pp. 109-119 ◽  
Author(s):  
S. J. Steinberg ◽  
A. Snowden ◽  
N. E. Braverman ◽  
L. Chen ◽  
P. A. Watkins ◽  
...  
Keyword(s):  
Cultured Fibroblasts

scholarly journals Activation of adenylate cyclase in cultured fibroblasts by trypsin.

1978 ◽  
Vol 253 (1) ◽  
pp. 24-26 ◽  
Author(s):  
D. Wallach ◽  
W. Anderson ◽  
I. Pastan
Keyword(s):  
Adenylate Cyclase ◽  
Cultured Fibroblasts
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