The Low PH Form of Redox-Active Components in the Donor Side of Photosystem II Studied by Pulsed EPR and ENDOR

1995 ◽  
pp. 559-562 ◽  
Author(s):  
Hiroyuki Mino ◽  
Andrei V. Astashkin ◽  
Asako Kawamori ◽  
Ono Taka-aki ◽  
Yorinao Inoue
Keyword(s):  
Photosystem Ii ◽  
Low Ph ◽  
Active Components ◽  
Donor Side ◽  
Pulsed Epr ◽  
Redox Active

scholarly journals High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Koji Kato ◽  
Naoyuki Miyazaki ◽  
Tasuku Hamaguchi ◽  
Yoshiki Nakajima ◽  
Fusamichi Akita ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Physiological State ◽  
High Dose ◽  
Final State ◽  
X Ray ◽  
Redox States ◽  
X Ray Crystallography ◽  
Cryo Electron Microscopy ◽  
Redox Active ◽  
Beam Damage

AbstractPhotosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the crystalline state and its final state structure has not been solved. Here we analyzed the structure of PSII in solution at 1.95 Å resolution by single-particle cryo-electron microscopy (cryo-EM). The structure obtained is similar to the crystal structure, but a PsbY subunit was visible in the cryo-EM structure, indicating that it represents its physiological state more closely. Electron beam damage was observed at a high-dose in the regions that were easily affected by redox states, and reducing the beam dosage by reducing frames from 50 to 2 yielded a similar resolution but reduced the damage remarkably. This study will serve as a good indicator for determining damage-free cryo-EM structures of not only PSII but also all biological samples, especially redox-active metalloproteins.

Pulsed EPR Study of Manganese g = 4.1 Signal in Plant Photosystem II

1994 ◽  
Vol 105 (2) ◽  
pp. 113-119 ◽  
Author(s):  
A.V. Astashkin ◽  
Y. Kodera ◽  
A. Kawamori
Keyword(s):  
Photosystem Ii ◽  
Pulsed Epr

scholarly journals Bicarbonate-reversible formate inhibition at the donor side of Photosystem II

1996 ◽  
Vol 1273 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Hanna Wincencjusz ◽  
Suleyman I. Allakhverdiev ◽  
Vyacheslav V. Klimov ◽  
Hans J. van Gorkom
Keyword(s):  
Photosystem Ii ◽  
Donor Side

pH Dependence of the Donor Side Reactions in Ca2+-Depleted Photosystem II†

Biochemistry ◽  
2003 ◽  
Vol 42 (20) ◽  
pp. 6185-6192 ◽  
Author(s):  
Stenbjörn Styring ◽  
Yashar Feyziyev ◽  
Fikret Mamedov ◽  
Warwick Hillier ◽  
Gerald T. Babcock
Keyword(s):  
Photosystem Ii ◽  
Ph Dependence ◽  
Side Reactions ◽  
Donor Side
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