A substrate cycling model for nitrate uptake by Pisum sativum seedlings: A key to sensitivity of response of net flux to substrate and effectors?

Author(s):  
Celia E. Deane-Drummond
1985 ◽  
Vol 227 (1) ◽  
pp. 299-304 ◽  
Author(s):  
T ap Rees ◽  
J H Green ◽  
P M Wilson

The activity of pyrophosphate:fructose-6-phosphate 1-phosphotransferase [PFK (PPi); EC 2.7.1.90] in extracts of the storage tissues of leek (Allium porrum), beetroot (Beta vulgaris) and roots of darnel (Lolium temulentum) exceeded 0.15 mumol/min per g fresh wt. As net flux from fructose 1,6-bisphosphate to fructose 6-phosphate in these tissues is unlikely, it is suggested that PFK (PPi) does not contribute to gluconeogenesis or starch synthesis. The maximum catalytic activities of PFK (PPi) in apex, stele and cortex of the root of pea (Pisum sativum) and in the developing and the thermogenic club of the spadix of cuckoo-pint (Arum maculatum) were measured and compared with those of phosphofructokinase, and to estimates of the rates of carbohydrate oxidation. PPi and fructose 2,6-bisphosphate in Arum clubs were measured. The above measurements are consistent with a glycolytic role for PFK (PPi) in tissues where there is marked biosynthesis, but not in the thermogenic club of Arum. The possibility that PFK (PPi) is a means of synthesizing pyrophosphate is discussed.


Botany ◽  
2018 ◽  
Vol 96 (3) ◽  
pp. 201-208 ◽  
Author(s):  
B.J. Hawkins ◽  
E. May ◽  
S. Robbins

Mosses play key ecological roles in water and nutrient retention in many ecosystems, yet relatively little is known of the functional characteristics of moss species, particularly nutritional characteristics. We investigated the net flux of ammonium, nitrate, and protons, using a microelectrode ion flux measurement system, in the gametophytes of 21 common species of moss from three contrasting locations in southern coastal British Columbia. The general location from which mosses were collected did not significantly affect ammonium or nitrate uptake. Proton efflux was greatest in mosses from locations with high rainfall. Rates of nitrate uptake differed among moss families, but there were no significant differences in uptake among species within families. Ammonium net flux differed among moss families, but also among species nested within family, with some species showing uptake and other showing ammonium efflux. In general, moss species native to dry habitats appeared to have higher rates of nitrogen uptake when ammonium and nitrate were available under favourable conditions.


2000 ◽  
Vol 148 (11-12) ◽  
pp. 591-595 ◽  
Author(s):  
U. P. Singh ◽  
B. Prithiviraj ◽  
B. K. Sarma

Author(s):  
Angang Li ◽  
Susana Bernal ◽  
Brady Kohler ◽  
Steven A. Thomas ◽  
Eugènia Martí ◽  
...  

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