Molecular determinants for resistance to crop protection chemicals

Author(s):  
D. W. Hollomon ◽  
J. A. Butters
Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.


2020 ◽  
Vol 477 (1) ◽  
pp. 173-189 ◽  
Author(s):  
Marco Pedretti ◽  
Carolina Conter ◽  
Paola Dominici ◽  
Alessandra Astegno

Arabidopsis centrin 2, also known as calmodulin-like protein 19 (CML19), is a member of the EF-hand superfamily of calcium (Ca2+)-binding proteins. In addition to the notion that CML19 interacts with the nucleotide excision repair protein RAD4, CML19 was suggested to be a component of the transcription export complex 2 (TREX-2) by interacting with SAC3B. However, the molecular determinants of this interaction have remained largely unknown. Herein, we identified a CML19-binding site within the C-terminus of SAC3B and characterized the binding properties of the corresponding 26-residue peptide (SAC3Bp), which exhibits the hydrophobic triad centrin-binding motif in a reversed orientation (I8W4W1). Using a combination of spectroscopic and calorimetric experiments, we shed light on the SAC3Bp–CML19 complex structure in solution. We demonstrated that the peptide interacts not only with Ca2+-saturated CML19, but also with apo-CML19 to form a protein–peptide complex with a 1 : 1 stoichiometry. Both interactions involve hydrophobic and electrostatic contributions and include the burial of Trp residues of SAC3Bp. However, the peptide likely assumes different conformations upon binding to apo-CML19 or Ca2+-CML19. Importantly, the peptide dramatically increases the affinity for Ca2+ of CML19, especially of the C-lobe, suggesting that in vivo the protein would be Ca2+-saturated and bound to SAC3B even at resting Ca2+-levels. Our results, providing direct evidence that Arabidopsis SAC3B is a CML19 target and proposing that CML19 can bind to SAC3B through its C-lobe independent of a Ca2+ stimulus, support a functional role for these proteins in TREX-2 complex and mRNA export.


Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381 ◽  
Author(s):  
A Bruguière ◽  
AM Le Ray ◽  
D Bréard ◽  
N Blon ◽  
N Bataillé ◽  
...  

2020 ◽  
Vol 31 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Fernando K. Carvalho ◽  
Rodolfo G. Chechetto ◽  
Alisson A. B. Mota ◽  
Ulisses R. Antuniassi

Crop protection on major crops is now required to follow the principles of integrated pest management so the timing and accuracy of any application of a pesticide or biopesticide has to be more precise to minimize adverse effects on non-target species. The development of UAVs (unmanned aerial vehicles) provides a means of providing a more targeted application of the correct dose, especially by using formulations that are more persistent, thus minimizing loss of spray in areas subject to rain. Avoiding use of too high a dosage allows greater survival of natural enemies and reduces the selection pressure for pests becoming resistant to specific modes of action. The downward flow of air from a UAV should also provide better distribution and impaction of droplets within a crop canopy, reduce soil impaction caused by taking heavy loads of spray applied with 200 l ha–1 of water, and allow treatments when fields are too wet to access with ground equipment. In Asia, many smallholder farmers are using a drone in preference to using a knapsack sprayer. According to Matthews, it has been shown that ULV spraying can be effective, but it needs a narrow droplet spectrum with the droplets remaining stable and not shrinking to become too small. Formulation research can reduce the volatility of the spray, hence the success of oil-based sprays. However, instead of petroleum-based oils, there is a chance to develop vegetable oil carriers with micro-sized particle suspensions to deliver low toxicity pesticides in droplets that can be deposited within the crop and not drift beyond the crop boundary. Oil deposits will be less prone to loss after rain so less should be lost in neighbouring ditches and water courses, especially as rainfall patterns are forecast to change. More studies are needed to evaluate the swath for deposition, buffer zones, formulation, nozzle selection, to guide future specific legislation for UAV applications.


2018 ◽  
Vol 29 (3) ◽  
pp. 102-103
Author(s):  
Ken Pallett

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