Consumers’ Willingness to Buy CRISPR Gene-Edited Tomatoes: Evidence from a Choice Experiment Case Study in Germany

The CRISPR gene-editing (GE) breeding method is used to increase the resilience of high-yielding tomato cultivars against pests and diseases, reducing crop protection requirements. This study investigated consumers’ willingness to buy CRISPR GE tomatoes in a repeated discrete-choice experiment. We observed a strong positive effect of providing information on the CRISPR breeding technology, while the sensory experience of the CRISPR GE tomatoes in a visit to a greenhouse had a rather weak, predominantly negative effect on the participants’ willingness to buy CRISPR GE tomatoes. We found that roughly half of the 32 participants demonstrated constant CRISPR GE tomato choices during the experiments, and these participants were mainly employed as scientists. However, the rest of the participants changed their CRISPR GE tomato choices, with the majority showing an increase in their willingness to buy CRISPR GE tomatoes; these “changers” were dominated by non-scientists. Science communication on CRISPR GE breeding technology should target people with little knowledge about the technology, and consumers of organic tomatoes seem to have more specified, stable preferences regarding the technology. Further, scientific information about the CRISPR GE methodology should preferentially be provided when new technology and information about it are not yet widespread and people have not yet formed a strong opinion about the technology.

Short-Term Impact of Multi-Cropping on Some Soil Physical Properties and Respiration

Growing as much crop biomass as possible in the shortest possible time is the target for most bio-energy producers. However, according to the requirements of the Green Deal, the consumption of fertilizers and crop protection products will have to be significantly reduced between 2023 and 2027. In order to meet all the necessary conditions for the production of biomass, a stationary field experiment was carried out at the Experimental Station of Vytautas Magnus University, Lithuania, in 2020–2021. Multi-cultivations of maize, hemp and faba bean were investigated. The aim of this study was to ascertain the impact of multi-cropping intensity on soil structural composition, stability, penetration resistance and gas concentration–respiration. As expected, multi-cropping stabilized the gas concentration and emission from the soil and decreased the proportion of micro-structures in the top soil layers. However, the stability of the soil decreased in all the experimental plots. Gas concentration and respiration mainly depended on soil structural composition, temperature and moisture content. The results of the experiment suggest performing investigations at a long-term scale because the intensive variation of meteorological conditions had a higher impact on the soil properties than the multi-cropping systems.

Vine Weevil, Otiorhynchus sulcatus (Coleoptera: Curculionidae), Management: Current State and Future Perspectives

Vine weevil, also known as black vine weevil, Otiorhynchus sulcatus, has been one of the most economically important pest species of global horticultural crops for the past five decades. This period has seen many changes in crop protection practices, including wide-scale adoption of biological controls such as entomopathogenic nematodes and fungi in place of conventional synthetic insecticides. Despite the experimental efficacy of these controls, growers continue to report significant crop losses associated with vine weevil infestation. We argue that simply switching from synthetic insecticides to biological controls, rather than using these controls as part of an integrated management program, is a key factor in the continued importance of this pest. An improved understanding of vine weevil biology and ecology is at the center of the development of truly integrated pest management programs. To this end, we identify opportunities created through recent vine weevil research and highlight key knowledge gaps in which further research may contribute to improved future management approaches.

Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt

The compound negative impact of insect pests attacking agricultural ecosystems includes (i) direct yield losses from damaged crops, (ii) the economic cost of the attempt to prevent these losses and (iii) the negative short- and long-term hazard effects of chemical pesticides on human and environmental health. Entomopathogenic fungi (EMPF) are a group of microorganisms that represent the natural enemies of a number of crop pests, presenting an opportunity to harness their evolutionary fine-tuned relationship with their insect hosts as biocontrol agents in integrated pest management programs. The aim of this study was to establish an indigenous EMPF collection via the Galleria mellonella (greater wax moth) entrapment method from the soils of Nile Delta, Egypt. Obtained insect associated fungal isolates were bio-assayed for pathogenicity against the serious pest Spodoptera litura and Tenebrio molitor, and the seven outperforming isolates were selected for molecular identification and thermotolerance assay. Based on ITS sequence analysis and phylogeny, selected isolates were identified as Beauveria bassiana (four isolates), Metarhizium anisopliae (two isolates) and one isolate of Cordyceps javanica. The obtained results demonstrated (i) the efficacy of using insect baiting coupled with molecular identification and pathogenicity screening to isolate EMPF to control insect pests, and (ii) the availability of indigenous virulent EMPF in Nile Delta’s soil, which can be exploited for the development of sustainable crop protection strategies.

Nano-pesticides: the lunch-box principle—deadly goodies (semio-chemical functionalised nanoparticles that deliver pesticide only to target species)

Nature contains many examples of “fake promises” to attract “prey”, e.g., predatory spiders that emit the same sex-attractant-signals as moths to catch them at close range and male spiders that make empty silk-wrapped gifts in order to mate with a female. Nano-pesticides should ideally mimic nature by luring a target and killing it without harming other organisms/species. Here, we present such an approach, called the lunch-box or deadly-goodies approach. The lunch-box consists of three main elements (1) the lure (semio-chemicals anchored on the box), (2) the box (palatable nano-carrier), and (3) the kill (advanced targeted pesticide). To implement this approach, one needs to draw on the vast amount of chemical ecological knowledge available, combine this with recent nanomaterial techniques, and use novel advanced pesticides. Precision nano-pesticides can increase crop protection and food production whilst lowering environmental impacts. Graphical Abstract

Coordinated Epigenetic Regulation in Plants: A Potent Managerial Tool to Conquer Biotic Stress

Plants have evolved variable phenotypic plasticity to counteract different pathogens and pests during immobile life. Microbial infection invokes multiple layers of host immune responses, and plant gene expression is swiftly and precisely reprogramed at both the transcriptional level and post-transcriptional level. Recently, the importance of epigenetic regulation in response to biotic stresses has been recognized. Changes in DNA methylation, histone modification, and chromatin structures have been observed after microbial infection. In addition, epigenetic modifications may be preserved as transgenerational memories to allow the progeny to better adapt to similar environments. Epigenetic regulation involves various regulatory components, including non-coding small RNAs, DNA methylation, histone modification, and chromatin remodelers. The crosstalk between these components allows precise fine-tuning of gene expression, giving plants the capability to fight infections and tolerant drastic environmental changes in nature. Fully unraveling epigenetic regulatory mechanisms could aid in the development of more efficient and eco-friendly strategies for crop protection in agricultural systems. In this review, we discuss the recent advances on the roles of epigenetic regulation in plant biotic stress responses.

Cotton in Southern Africa.

This chapter focuses on the crop protection and pest management of cotton crops in Southern Africa (Eswatini, Zimbabwe, Zambia, Malawi, Mozambique, and Angola). It discusses how new technology will bring major changes in how cotton is grown in the future.