Detection of Membrane Mechanical Properties and Endocytosis by Single Molecule Force Spectroscopy

2017 ◽  
pp. 91-115
Author(s):  
Yuping Shan
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Membrane Mechanical Properties

Effect of chemical cross-linking on the mechanical properties of elastomeric peptides studied by single molecule force spectroscopy

2011 ◽  
Vol 44 (11) ◽  
pp. 2118-2122 ◽  
Author(s):  
Francesca Sbrana ◽  
Marina Lorusso ◽  
Claudio Canale ◽  
Brigida Bochicchio ◽  
Massimo Vassalli
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Cross Linking ◽  
Single Molecule Force Spectroscopy ◽  
Chemical Cross Linking

Deciphering the Mechanical Properties of Type III Secretion System EspA Protein by Single Molecule Force Spectroscopy

Langmuir ◽  
2018 ◽  
Vol 34 (21) ◽  
pp. 6261-6270 ◽  
Author(s):  
Hila Nadler ◽  
Lihi Shaulov ◽  
Yossi Blitsman ◽  
Moran Mordechai ◽  
Jürgen Jopp ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Force Spectroscopy ◽  
Secretion System ◽  
Single Molecule Force Spectroscopy ◽  
Type Iii

scholarly journals Bioconjugation Strategies for Connecting Proteins to DNA-Linkers for Single-Molecule Force-Based Experiments

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2424
Author(s):  
Lyan M. van der Sleen ◽  
Katarzyna M. Tych
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Short Range ◽  
Force Spectroscopy ◽  
Magnetic Tweezers ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of proteins can be studied with single molecule force spectroscopy (SMFS) using optical tweezers, atomic force microscopy and magnetic tweezers. It is common to utilize a flexible linker between the protein and trapped probe to exclude short-range interactions in SMFS experiments. One of the most prevalent linkers is DNA due to its well-defined properties, although attachment strategies between the DNA linker and protein or probe may vary. We will therefore provide a general overview of the currently existing non-covalent and covalent bioconjugation strategies to site-specifically conjugate DNA-linkers to the protein of interest. In the search for a standardized conjugation strategy, considerations include their mechanical properties in the context of SMFS, feasibility of site-directed labeling, labeling efficiency, and costs.

Using single molecule force spectroscopy to facilitate a rational design of Ca2+-responsive β-roll peptide-based hydrogels

2018 ◽  
Vol 6 (32) ◽  
pp. 5303-5312 ◽  
Author(s):  
Lichao Liu ◽  
Han Wang ◽  
Yueying Han ◽  
Shanshan Lv ◽  
Jianfeng Chen
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Rational Design ◽  
Mechanical Stability ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

Mechanical stability of Ca2+-responsive β-roll peptides (RTX) is largely responsible for the Ca2+-dependent mechanical properties of the RTX-based hydrogels.

Mechanical properties of ankyrin repeats examined with single-molecule force spectroscopy

2006 ◽  
Vol 39 ◽  
pp. S244
Author(s):  
G. Lee ◽  
K. Abdi ◽  
Y. Jiang ◽  
P. Michaely ◽  
V. Bennett ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Ankyrin Repeats ◽  
Single Molecule Force Spectroscopy

scholarly journals Mechanical properties of elastomeric proteins studied by single molecule force spectroscopy

2016 ◽  
Vol 65 (18) ◽  
pp. 188703
Author(s):  
Zhou Hao-Tian ◽  
Gao Xiang ◽  
Zheng Peng ◽  
Qin Meng ◽  
Cao Yi ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

scholarly journals Distinct kinetic and mechanical properties govern ALCAM-mediated interactions as shown by single-molecule force spectroscopy

2007 ◽  
Vol 120 (22) ◽  
pp. 3965-3976 ◽  
Author(s):  
J. te Riet ◽  
A. W. Zimmerman ◽  
A. Cambi ◽  
B. Joosten ◽  
S. Speller ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy
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