Lipid Cubic Phase for Membrane Protein X-ray Crystallography

2017 ◽  
pp. 175-220 ◽  
Author(s):  
Jialu Zha ◽  
Dianfan Li
Keyword(s):  
Membrane Protein ◽  
Cubic Phase ◽  
X Ray ◽  
X Ray Crystallography ◽  
Protein X

A study of membrane lipids from dehydration-acclimated Brassica napus root cells: formation of a cubic phase under physiological conditions

1990 ◽  
Vol 68 (1) ◽  
pp. 102-105 ◽  
Author(s):  
P. Norberg ◽  
K. Larsson ◽  
C. Liljenberg
Keyword(s):  
Water Deficit ◽  
Membrane Lipids ◽  
Phase Behaviour ◽  
Water Deficit Stress ◽  
Cubic Phase ◽  
Polarization Microscopy ◽  
Root Cells ◽  
X Ray ◽  
X Ray Crystallography ◽  
Microsomal Membranes

Rape seedlings were acclimated to evaporative dehydration by exposure to repeated moderate water-deficit stress. The stress program started after 19 days of growth and consisted of three, 24-h stress periods interspersed with 24-h rewatering periods. After the third stress period the roots were harvested and microsomal membranes were isolated. Control plants were grown under equivalent conditions without stress (nonacclimated cells). Total lipids were extracted from the membranes and investigated with X-ray crystallography and polarization microscopy at different degrees of hydration and temperatures. In excess water, the membrane lipids from both acclimated and nonacclimated cells exhibited a cubic phase. The lipids from the nonacclimated cells formed a hexagonal (HII) phase on dehydration. The lipids from the acclimated cells behaved in a different way during dehydration, where the cubic phase was transformed to an L2 phase via an intermediate HII phase. At increasing temperatures, the hydrated cubic phase started to form an L2 phase at 30 °C and was fully converted to the liquid-type state at 42 °C. The mesomorphic phase behaviour is discussed in relation to membrane activity.Key words: water-deficit stress, microsomal membranes, X-ray crystallography, polarization microscopy.

Three-dimensional crystallization of membrane proteins

1988 ◽  
Vol 21 (4) ◽  
pp. 429-477 ◽  
Author(s):  
W. Kühlbrandt
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Complexes ◽  
Three Dimensional ◽  
Biological Macromolecules ◽  
X Ray ◽  
X Ray Crystallography ◽  
Membrane Protein Complexes ◽  
Essential Prerequisite

As recently as 10 years ago, the prospect of solving the structure of any membrane protein by X-ray crystallography seemed remote. Since then, the threedimensional (3-D) structures of two membrane protein complexes, the bacterial photosynthetic reaction centres of Rhodopseudomonas viridis (Deisenhofer et al. 1984, 1985) and of Rhodobacter sphaeroides (Allen et al. 1986, 1987 a, 6; Chang et al. 1986) have been determined at high resolution. This astonishing progress would not have been possible without the pioneering work of Michel and Garavito who first succeeded in growing 3-D crystals of the membrane proteins bacteriorhodopsin (Michel & Oesterhelt, 1980) and matrix porin (Garavito & Rosenbusch, 1980). X-ray crystallography is still the only routine method for determining the 3-D structures of biological macromolecules at high resolution and well-ordered 3-D crystals of sufficient size are the essential prerequisite.

Protein X-ray Crystallography in Drug Discovery

2005 ◽  
pp. 373-455 ◽  
Author(s):  
Peter Nollert ◽  
Michael D. Feese ◽  
Bart L. Staker ◽  
Hidong Kim
Keyword(s):  
Drug Discovery ◽  
X Ray ◽  
X Ray Crystallography ◽  
Protein X

scholarly journals High-Pressure Protein X-Ray Crystallography

2017 ◽  
Vol 27 (1) ◽  
pp. 18-25
Author(s):  
Nobuhisa WATANABE ◽  
Hiroyuki YAMADA ◽  
Takayuki NAGAE
Keyword(s):  
High Pressure ◽  
X Ray ◽  
X Ray Crystallography ◽  
Protein X

Principles of Protein X-Ray Crystallography. Jan Drenth

2000 ◽  
Vol 75 (1) ◽  
pp. 48-48
Author(s):  
Gregory A. Petsko
Keyword(s):  
X Ray ◽  
X Ray Crystallography ◽  
Protein X

Incorporation of β-selenolo[3,2-b]pyrrolyl-alanine into proteins for phase determination in protein X-ray crystallography

2001 ◽  
Vol 309 (4) ◽  
pp. 925-936 ◽  
Author(s):  
Jae Hyun Bae ◽  
Stefan Alefelder ◽  
Jens T Kaiser ◽  
Rainer Friedrich ◽  
Luis Moroder ◽  
...  
Keyword(s):  
Phase Determination ◽  
X Ray ◽  
X Ray Crystallography ◽  
Protein X
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