scholarly journals Single-Cell Cultivation Utilizing Microfluidic Systems

2021 ◽  
pp. 287-310
Author(s):  
Dian Anggraini ◽  
Nobutoshi Ota ◽  
Yigang Shen ◽  
Yo Tanaka ◽  
Yoichiroh Hosokawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Cultivation ◽  
Microfluidic Systems

Single-Cell Cultivation Utilizing Microfluidic Systems

2020 ◽  
pp. 1-24
Author(s):  
Dian Anggraini ◽  
Nobutoshi Ota ◽  
Yigang Shen ◽  
Yo Tanaka ◽  
Yoichiroh Hosokawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Cultivation ◽  
Microfluidic Systems

scholarly journals Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

2020 ◽  
Vol 11 (25) ◽  
pp. 6352-6361 ◽  
Author(s):  
Kaixiang Zhang ◽  
Shangshang Qin ◽  
Sixuan Wu ◽  
Yan Liang ◽  
Jinghong Li
Keyword(s):  
Single Cell ◽  
Antibiotic Treatment ◽  
Single Molecule ◽  
Antibiotic Susceptibility ◽  
Single Cell Level ◽  
Microfluidic Systems ◽  
Cell Level ◽  
Single Molecule Level ◽  
Recent Developments ◽  
Susceptibility Tests

Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.

scholarly journals New Approaches for Isolation of Previously Uncultivated Oral Bacteria

2011 ◽  
Vol 78 (1) ◽  
pp. 194-203 ◽  
Author(s):  
M. V. Sizova ◽  
T. Hohmann ◽  
A. Hazen ◽  
B. J. Paster ◽  
S. R. Halem ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Single Cell ◽  
Single Species ◽  
Oral Bacteria ◽  
Cell Cultivation ◽  
Human Oral Cavity ◽  
Successful Recovery ◽  
Number Of Microorganisms ◽  
Enrichment Techniques

ABSTRACTA significant number of microorganisms from the human oral cavity remain uncultivated. This is a major impediment to the study of human health since some of the uncultivated species may be involved in a variety of systemic diseases. We used a range of innovations previously developed to cultivate microorganisms from the human oral cavity, focusing on anaerobic species. These innovations include (i)in vivocultivation to specifically enrich for species actively growing in the oral cavity (the “minitrap” method), (ii) single-cell long-term cultivation to minimize the effect of fast-growing microorganisms, and (iii) modifications of conventional enrichment techniques, using media that did not contain sugar, including glucose. To enable cultivation of obligate anaerobes, we maintained strict anaerobic conditions in most of our cultivation experiments. We report that, on a per cell basis, the most successful recovery was achieved using minitrap enrichment (11%), followed by single-cell cultivation (3%) and conventional plating (1%). Taxonomically, the richest collection was obtained using the single-cell cultivation method, followed by minitrap and conventional enrichment, comprising representatives of 13, 9, and 4 genera, respectively. Interestingly, no single species was isolated by all three methods, indicating method complementarity. An important result is the isolation and maintenance in pure culture of 10 strains previously only known by their molecular signatures, as well as representatives of what are likely to be three new microbial genera. We conclude that the ensemble of new methods we introduced will likely help close the gap between cultivated and uncultivated species from the human oral cavity.

Measurement of Incident Angle Dependence of Swimming Bacterium Reflection Using On-Chip Single-Cell Cultivation Assay

2003 ◽  
Vol 42 (Part 2, No. 7B) ◽  
pp. L873-L875 ◽  
Author(s):  
Akihiro Hattori ◽  
Senkei Umehara ◽  
Yuichi Wakamoto ◽  
Kenji Yasuda
Keyword(s):  
Single Cell ◽  
Incident Angle ◽  
Cell Cultivation ◽  
Angle Dependence ◽  
On Chip

scholarly journals Coarse-grained simulation for the analysis of microfluidic single-cell cultivation

2018 ◽  
Vol 90 (9) ◽  
pp. 1265-1265
Author(s):  
C. Westerwalbesloh ◽  
D. Kohlheyer ◽  
E. von Lieres
Keyword(s):  
Single Cell ◽  
Coarse Grained ◽  
Cell Cultivation

Robotic and microfluidic systems for single cell injection

2021 ◽  
pp. 241-260
Author(s):  
Peng Pan ◽  
Yuxiao Zhou ◽  
Zhaoyi Xu ◽  
Yu Sun ◽  
Xinyu Liu
Keyword(s):  
Single Cell ◽  
Microfluidic Systems ◽  
Cell Injection

scholarly journals Automated Chemotactic Sorting and Single-cell Cultivation of Microbes using Droplet Microfluidics

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Libing Dong ◽  
Dong-Wei Chen ◽  
Shuang-Jiang Liu ◽  
Wenbin Du
Keyword(s):  
Single Cell ◽  
Droplet Microfluidics ◽  
Cell Cultivation
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