scholarly journals Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

2020 ◽  
Vol 11 (25) ◽  
pp. 6352-6361 ◽  
Author(s):  
Kaixiang Zhang ◽  
Shangshang Qin ◽  
Sixuan Wu ◽  
Yan Liang ◽  
Jinghong Li
Keyword(s):  
Single Cell ◽  
Antibiotic Treatment ◽  
Single Molecule ◽  
Antibiotic Susceptibility ◽  
Single Cell Level ◽  
Microfluidic Systems ◽  
Cell Level ◽  
Single Molecule Level ◽  
Recent Developments ◽  
Susceptibility Tests

Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.

Microfluidic screening of antibiotic susceptibility at a single-cell level shows the inoculum effect of cefotaxime on E. coli

Lab on a Chip ◽  
2018 ◽  
Vol 18 (23) ◽  
pp. 3668-3677 ◽  
Author(s):  
Witold Postek ◽  
Pawel Gargulinski ◽  
Ott Scheler ◽  
Tomasz S. Kaminski ◽  
Piotr Garstecki
Keyword(s):  
Single Cell ◽  
Antibiotic Susceptibility ◽  
Single Cell Level ◽  
Cell Level ◽  
Reaction Conditions ◽  
E Coli ◽  
Inoculum Effect

We separate emulsions with an immiscible oil phase to identify reaction conditions by the location of emulsion in emulsion series.

scholarly journals Investigating DNA Replication in Escherichia Coli on the Single Cell Level Utilizing Microfluidics and Single-Molecule Fluorescence Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 368a-369a
Author(s):  
Charl Moolman ◽  
Sriram T. Krishnan ◽  
Jacob W.K. Kerssemakers ◽  
Susanne Hage ◽  
Rodrigo Reyes-Lamothe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Single Cell Level ◽  
Single Molecule Fluorescence ◽  
Cell Level ◽  
Single Molecule Fluorescence Microscopy

Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level

2017 ◽  
pp. 109-121 ◽  
Author(s):  
Niek Welkenhuysen ◽  
Caroline B. Adiels ◽  
Mattias Goksör ◽  
Stefan Hohmann
Keyword(s):  
Single Cell ◽  
Single Cell Level ◽  
Microfluidic Systems ◽  
Cell Level

scholarly journals Investigating the Dynamics of the β2 Sliding Clamp in Escherichia Coli at the Single-Cell Level Utilizing Single-Molecule Fluorescence Microscopy

2014 ◽  
Vol 106 (2) ◽  
pp. 273a
Author(s):  
Sriram Tiruvadi Krishnan ◽  
Martin Charl Moolman ◽  
Jacob W.J. Kerssemakers ◽  
Theo van Laar ◽  
Pawel Tulinski ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Single Cell Level ◽  
Single Molecule Fluorescence ◽  
Cell Level ◽  
Sliding Clamp ◽  
Single Molecule Fluorescence Microscopy

scholarly journals Single-molecule long-read sequencing reveals the chromatin basis of gene expression

2019 ◽  
Author(s):  
Yunhao Wang ◽  
Anqi Wang ◽  
Zujun Liu ◽  
Andrew Thurman ◽  
Linda S. Powers ◽  
...  
Keyword(s):  
Single Cell ◽  
Long Range ◽  
Single Molecule ◽  
Single Cells ◽  
Chromatin Accessibility ◽  
Single Cell Level ◽  
Dna Molecules ◽  
Cell Level ◽  
Single Dna Molecules ◽  
Long Read

ABSTRACTGenome-wide chromatin accessibility and nucleosome occupancy profiles have been widely investigated, while the long-range dynamics remains poorly studied at the single-cell level. Here we present a new experimental approach MeSMLR-seq (methyltransferase treatment followed by single-molecule long-read sequencing) for long-range mapping of nucleosomes and chromatin accessibility at single DNA molecules, and thus achieve comprehensive-coverage characterization of the corresponding heterogeneity. We applied MeSMLR-seq to haploid yeast, where single DNA molecules represent single cells, and thus we could investigate the combinatorics of many (up to 356) nucleosomes at long range in single cells. We illustrated the differential organization principles of nucleosomes surrounding transcription start site for silently- and actively-transcribed genes, at the single-cell level and in the long-range scale. The heterogeneous patterns of chromatin statuses spanning multiple genes were phased. Together with single-cell RNA-seq data, we quantitatively revealed how chromatin accessibility correlated with gene transcription positively in a highly-heterogeneous scenario. Moreover, we quantified the openness of promoters and investigated the coupled chromatin changes of adjacent genes at single DNA molecules during transcription reprogramming.

scholarly journals Single-Cell Analysis of Multiple Steps of Dynamic NF-κB Regulation in Interleukin-1α-Triggered Tumor Cells Using Proximity Ligation Assays

Cancers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1199 ◽  
Author(s):  
Mayr-Buro ◽  
Schlereth ◽  
Beuerlein ◽  
Tenekeci ◽  
Meier-Soelch ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Molecule ◽  
Nuclear Translocation ◽  
Single Cell Analysis ◽  
Single Cell Level ◽  
Cell Level ◽  
Dynamic Regulation ◽  
Proximity Ligation ◽  
Body Component ◽  
Almost All

The frequently occurring heterogeneity of cancer cells and their functional interaction with immune cells in the tumor microenvironment raises the need to study signaling pathways at the single cell level with high precision, sensitivity, and spatial resolution. As aberrant NF-κB activity has been implicated in almost all steps of cancer development, we analyzed the dynamic regulation and activation status of the canonical NF-κB pathway in control and IL-1α-stimulated individual cells using proximity ligation assays (PLAs). These systematic experiments allowed the visualization of the dynamic dissociation and re-formation of endogenous p65/IκBα complexes and the nuclear translocation of NF-κB p50/p65 dimers. PLA combined with immunostaining for p65 or with NFKBIA single molecule mRNA-FISH facilitated the analysis of (i) further levels of the NF-κB pathway, (i) its functionality for downstream gene expression, and (iii) the heterogeneity of the NF-κB response in individual cells. PLA also revealed the interaction between NF-κB p65 and the P-body component DCP1a, a new p65 interactor that contributes to efficient p65 NF-κB nuclear translocation. In summary, these data show that PLA technology faithfully mirrored all aspects of dynamic NF-κB regulation, thus allowing molecular diagnostics of this key pathway at the single cell level which will be required for future precision medicine.

Deciphering Molecular Complexity in Dynamics and Kinetics—From the Single Molecule to the Single Cell Level

2019 ◽  
Vol 123 (30) ◽  
pp. 6387-6388
Author(s):  
Tamiki Komatsuzaki ◽  
Steve Pressé ◽  
Patrick Senet
Keyword(s):  
Single Cell ◽  
Single Molecule ◽  
Single Cell Level ◽  
Cell Level ◽  
Molecular Complexity
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