Recent Advances in Molecular Diagnostic Approaches for Cancer

2019 ◽  
pp. 1-9
Author(s):  
Kamla Kant Shukla ◽  
Shrimanjunath Sankanagoudar ◽  
Barkha Singhal Sanganeria ◽  
Puneet Pareek ◽  
Jeevan Ram ◽  
...  
Keyword(s):  
Molecular Diagnostic ◽  
Recent Advances ◽  
Diagnostic Approaches

Molecular Diagnostic Approaches and Their Clinical Utility

2017 ◽  
pp. 65-77
Author(s):  
Laura N. Purcell ◽  
Paula D. Strassle ◽  
Jen Jen Yeh
Keyword(s):  
Clinical Utility ◽  
Molecular Diagnostic ◽  
Diagnostic Approaches

Molecular diagnostic approaches for haemorrhagic septicaemia[HS]: A Review

2017 ◽  
Vol 38 (2) ◽  
pp. 51
Author(s):  
S.B. Shivachandra ◽  
M.M. Chanda ◽  
J. Hiremath ◽  
R. Yogisharadhya ◽  
N.N. Mohanty ◽  
...  
Keyword(s):  
Molecular Diagnostic ◽  
Haemorrhagic Septicaemia ◽  
Diagnostic Approaches

Ethylmalonic encephalopathy: application of improved biochemical and molecular diagnostic approaches

2011 ◽  
Vol 79 (4) ◽  
pp. 385-390 ◽  
Author(s):  
A Drousiotou ◽  
I DiMeo ◽  
R Mineri ◽  
Th Georgiou ◽  
G Stylianidou ◽  
...  
Keyword(s):  
Molecular Diagnostic ◽  
Ethylmalonic Encephalopathy ◽  
Diagnostic Approaches

scholarly journals Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations

Separations ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 204
Author(s):  
Rian Ka Praja ◽  
Wisitsak Phoksawat ◽  
Patcharaporn Tippayawat ◽  
Amonrat Jumnainsong ◽  
Chanvit Leelayuwat
Keyword(s):  
High Density Lipoprotein ◽  
Protein Pattern ◽  
Density Lipoprotein ◽  
Sample Volume ◽  
Molecular Diagnostic ◽  
Particle Sizes ◽  
Limited Sample ◽  
Modified Method ◽  
Diagnostic Approaches ◽  
Commercial Kits

High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with various small serum volumes. HDL was isolated from 200 µL (HDL200) and 500 µL (HDL500) of sera. Three different volumes: 50 µL (Exo50), 100 µL (Exo100), and 250 µL (Exo250) were used for exosome isolation. HDL and exosomes were isolated using commercial kits with the modified method and characterized by multiple approaches. The HDL levels of HDL200 and HDL500 were not significantly different (p > 0.05), with percent recoveries of >90%. HDL200 and HDL500 had the same protein pattern with a biochemical similarity of 99.60 ± 0.10%. The particle sizes of Exo50, Exo100, and Exo250 were in the expected range. All isolated exosomes exhibited a similar protein pattern with a biochemical similarity of >99%. In conclusion, two different serum volumes (200 and 500 µL) and three different serum volumes (50, 100, and 250 µL) can be employed for HDL and exosome isolation, respectively. The possibility of HDL and exosome isolation with small volumes will accelerate biomarker discoveries with various molecular diagnostic approaches.

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