Methods for Single-Cell Isolation and Preparation

2020 ◽  
pp. 7-27
Author(s):  
Daniel Pensold ◽  
Geraldine Zimmer-Bensch
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Single Cell Isolation

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

Application Of Isoraft Single Cell Isolation For Analysis Of Pediatric Bal Macrophages

2014 ◽  
Vol 133 (2) ◽  
pp. AB142
Author(s):  
Neil Alexis ◽  
Heather Wells ◽  
Yogesh Saini ◽  
Louisa Brighton ◽  
Nancy Allbritton ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Single Cell Isolation

Grids from bands, or bands from grids? An examination of the effects of single unit contamination on grid cell firing fields

2016 ◽  
Vol 115 (2) ◽  
pp. 992-1002 ◽  
Author(s):  
Z. Navratilova ◽  
K. B. Godfrey ◽  
B. L. McNaughton
Keyword(s):  
Single Cell ◽  
Grid Cell ◽  
Cell Isolation ◽  
Recording Technology ◽  
Firing Patterns ◽  
Limiting Step ◽  
Isolation Methods ◽  
Single Cell Isolation ◽  
Cell Firing ◽  
Cell Data

Neural recording technology is improving rapidly, allowing for the detection of spikes from hundreds of cells simultaneously. The limiting step in multielectrode electrophysiology continues to be single cell isolation. However, this step is crucial to the interpretation of data from putative single neurons. We present here, in simulation, an illustration of possibly erroneous conclusions that may be reached when poorly isolated single cell data are analyzed. Grid cells are neurons recorded in rodents, and bats, that spike in equally spaced locations in a hexagonal pattern. One theory states that grid firing patterns arise from a combination of band firing patterns. However, we show here that summing the grid firing patterns of two poorly resolved neurons can result in spurious band-like patterns. Thus, evidence of neurons spiking in band patterns must undergo extreme scrutiny before it is accepted. Toward this aim, we discuss single cell isolation methods and metrics.

Clonal Isolation of Hybridomas by Manual Single-Cell Isolation

Hybridoma ◽  
1983 ◽  
Vol 2 (2) ◽  
pp. 231-234 ◽  
Author(s):  
R.J. SIJENS ◽  
A.A.M. THOMAS ◽  
A. JACKERS ◽  
A. BOEYÉ
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Single Cell Isolation

scholarly journals Advances in Single-Cell Printing

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Xiaohu Zhou ◽  
Han Wu ◽  
Haotian Wen ◽  
Bo Zheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Cell Isolation ◽  
Special Focus ◽  
Cell Analysis ◽  
Cell Array ◽  
Cell Printing ◽  
Recent Developments ◽  
Single Cell Isolation

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

scholarly journals In vitro differentiation of human oocyte-like cells from oogonial stem cells: single-cell isolation and molecular characterization

2018 ◽  
Vol 33 (3) ◽  
pp. 464-473 ◽  
Author(s):  
Erica Silvestris ◽  
Paola Cafforio ◽  
Stella D’Oronzo ◽  
Claudia Felici ◽  
Franco Silvestris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Molecular Characterization ◽  
Cell Isolation ◽  
Human Oocyte ◽  
In Vitro Differentiation ◽  
Single Cell Isolation

scholarly journals Imaging-assisted hydrogel formation for single cell isolation

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sander Oldenhof ◽  
Serhii Mytnyk ◽  
Alexandra Arranja ◽  
Marcel de Puit ◽  
Jan H. van Esch
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Hydrogel Formation ◽  
Single Cell Isolation

scholarly journals Subnanoliter precision piezo pipette for single-cell isolation and droplet printing

2020 ◽  
Vol 24 (2) ◽  
Author(s):  
Barbara Francz ◽  
Rita Ungai-Salánki ◽  
Éva Sautner ◽  
Robert Horvath ◽  
Bálint Szabó
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Single Cell Isolation

scholarly journals Single-cell isolation using a DVD optical pickup

2011 ◽  
Vol 19 (11) ◽  
pp. 10377 ◽  
Author(s):  
A. Kasukurti ◽  
M. Potcoava ◽  
S.A. Desai ◽  
C. Eggleton ◽  
D. W. M. Marr
Keyword(s):  
Single Cell ◽  
Cell Isolation ◽  
Optical Pickup ◽  
Single Cell Isolation

scholarly journals Isolation of Oligotrophic Denitrifiers Carrying Previously Uncharacterized Functional Gene Sequences

2010 ◽  
Vol 77 (1) ◽  
pp. 338-342 ◽  
Author(s):  
Satoshi Ishii ◽  
Naoaki Ashida ◽  
Shigeto Otsuka ◽  
Keishi Senoo
Keyword(s):  
16S Rrna ◽  
Single Cell ◽  
Nitrite Reductase ◽  
Cell Isolation ◽  
Taxonomic Composition ◽  
Functional Gene ◽  
Gene Sequences ◽  
Isolation Technique ◽  
Culture Independent ◽  
Single Cell Isolation

ABSTRACTOligotrophic denitrifying bacteria, including those belonging to the generaHerbaspirillum,Azospirillum, andBradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N2O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identifiedBradyrhizobiumstrains that harbornirSsequences previously detected only in culture-independent studies.

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