In Vitro Luminal Measurements of Colon Electrical Impedance in Rabbits

Author(s):  
Edelberto Mulett-Vásquez ◽  
Amilbia Correa-Florez ◽  
Cármen Dussán-Lubert ◽  
David-Alejandro Miranda-Mercado ◽  
Carlos-Augusto González-Correa
Keyword(s):  
Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1433
Author(s):  
Esther Tanumihardja ◽  
Douwe S. de Bruijn ◽  
Rolf H. Slaats ◽  
Wouter Olthuis ◽  
Albert van den Berg

A ruthenium oxide (RuOx) electrode was used to monitor contractile events of human pluripotent stem cells-derived cardiomyocytes (hPSC-CMs) through electrical impedance spectroscopy (EIS). Using RuOx electrodes presents an advantage over standard thin film Pt electrodes because the RuOx electrodes can also be used as electrochemical sensor for pH, O2, and nitric oxide, providing multisensory functionality with the same electrode. First, the EIS signal was validated in an optically transparent well-plate setup using Pt wire electrodes. This way, visual data could be recorded simultaneously. Frequency analyses of both EIS and the visual data revealed almost identical frequency components. This suggests both the EIS and visual data captured the similar events of the beating of (an area of) hPSC-CMs. Similar EIS measurement was then performed using the RuOx electrode, which yielded comparable signal and periodicity. This mode of operation adds to the versatility of the RuOx electrode’s use in in vitro studies.


NeuroImage ◽  
2017 ◽  
Vol 161 ◽  
pp. 104-119 ◽  
Author(s):  
Rosalind J. Sadleir ◽  
Fanrui Fu ◽  
Corey Falgas ◽  
Stephen Holland ◽  
May Boggess ◽  
...  

2021 ◽  
Vol 19 (3) ◽  
pp. 217-226
Author(s):  
G. Domínguez ◽  
E. Cardiel ◽  
J.L Reyes ◽  
E. Sánchez ◽  
P.R. Hernández

Purpose: The development of an electric impedance meter based on the impedance spectroscopy technique, for in vitro and in situ experimentation, with cellular epithelia submitted to extremely low frequency magnetic fields in a controlled environment. Unlike other reported systems, a strength of the one presented here is that it avoids the influence of external factors on the experiment. Materials and methods: The designed system employs the electrical impedance values obtained by the impedance spectroscopy technique to determine the parameters of the simple equivalent electrical model of a cellular monolayer. The Madin-Darby Canine Kidney (MDCK) cell cultures were used as subjects of study in the experimental protocol. Results: The validation was carried out by comparing the transepithelial electrical impedance data of the cell cultures obtained with the developed system and those of the Cellzscope® commercial system used as the standard. Non-significant differences were obtained. Conclusion: It was confirmed that the developed system provides reliable values of transepithelial electrical impedance to experiment with cell cultures and take advantage of the controlled environment to reduce the effects of experimental management.


Author(s):  
Amelie Degache ◽  
Florence Poulletier de Gannes ◽  
André Garenne ◽  
Rémy Renom ◽  
Yann Percherancier ◽  
...  

2008 ◽  
Vol 29 (3) ◽  
pp. 163-168 ◽  
Author(s):  
Berta Rivas ◽  
Pablo M. Botta ◽  
Purificación Varela ◽  
Benjamín Martín ◽  
Alfonso Fondado ◽  
...  

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