In vitro electrical impedance spectroscopy of human dentine: The effect of restorative materials

Aims: Oncology treatments aim at selective toxicity for tumor (compared to normal) cells, and chromone- coumarin hybrids have shown such activity. Methods: In this study, we test a novel series of synthetic chromone and coumarin derivatives (1–9) for cytotoxic activity against a panel of tumor cell lines (MCF-7, A549, HepG2, HTC-116, B16 and Caco-2) opposed to non-tumor cells (HEK-293t). Electrical impedance spectroscopy was used to monitor cell viability in real time. Results: Compound 8 showed the most potent activity, and it significantly diminished cancer cell proliferation and viability in different cell lines. It induced apoptosis in a dose-dependent manner, as shown by Western blot and flow cytometry. Conclusion: Electrical impedance spectroscopy appears to be a convenient tool for in vitro cytotoxicity analysis, which could be useful for identifying drug effects and side effects during early phases of drug discovery and development.

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In vitro analysis of various cell lines responses to electroporative electric pulses by means of electrical impedance spectroscopy

Biosensors and Bioelectronics ◽

10.1016/j.bios.2018.06.016 ◽

2018 ◽

Vol 117 ◽

pp. 207-216 ◽

Cited By ~ 9

Author(s):

Tomás García-Sánchez ◽

Ramon Bragós ◽

Lluis M. Mir

Keyword(s):

Impedance Spectroscopy ◽

Cell Lines ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Electric Pulses ◽

Vitro Analysis ◽

In Vitro Analysis

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Electrical Impedance Spectroscopy Estimation of Water Content in Leaves and Stems of White Birch (Betula platyphylla) Under Flooding Stress

CHINESE BULLETIN OF BOTANY ◽

10.3724/sp.j.1259.2014.00105 ◽

2014 ◽

Vol 49 (1) ◽

pp. 105 ◽

Cited By ~ 1

Author(s):

Meng Yu ◽

Gong Ruijuan ◽

Di Bao ◽

Xiang Diying ◽

Zhang Gang ◽

...

Keyword(s):

Impedance Spectroscopy ◽

Water Content ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Betula Platyphylla ◽

White Birch ◽

Flooding Stress

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Monitoring Contractile Cardiomyocytes via Impedance Using Multipurpose Thin Film Ruthenium Oxide Electrodes

Sensors ◽

10.3390/s21041433 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1433

Author(s):

Esther Tanumihardja ◽

Douwe S. de Bruijn ◽

Rolf H. Slaats ◽

Wouter Olthuis ◽

Albert van den Berg

Keyword(s):

Thin Film ◽

Electrical Impedance ◽

Ruthenium Oxide ◽

Electrical Impedance Spectroscopy ◽

Visual Data ◽

Oxide Electrodes ◽

Pt Electrodes ◽

Mode Of Operation ◽

Frequency Components

A ruthenium oxide (RuOx) electrode was used to monitor contractile events of human pluripotent stem cells-derived cardiomyocytes (hPSC-CMs) through electrical impedance spectroscopy (EIS). Using RuOx electrodes presents an advantage over standard thin film Pt electrodes because the RuOx electrodes can also be used as electrochemical sensor for pH, O2, and nitric oxide, providing multisensory functionality with the same electrode. First, the EIS signal was validated in an optically transparent well-plate setup using Pt wire electrodes. This way, visual data could be recorded simultaneously. Frequency analyses of both EIS and the visual data revealed almost identical frequency components. This suggests both the EIS and visual data captured the similar events of the beating of (an area of) hPSC-CMs. Similar EIS measurement was then performed using the RuOx electrode, which yielded comparable signal and periodicity. This mode of operation adds to the versatility of the RuOx electrode’s use in in vitro studies.

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A Rapid Approach to Measure Extracted Chlorophyll-a from Lettuce Leaves using Electrical Impedance Spectroscopy

Water Air & Soil Pollution ◽

10.1007/s11270-021-05036-z ◽

2021 ◽

Vol 232 (2) ◽

Author(s):

Rakibul Islam Chowdhury ◽

Rinku Basak ◽

Khan Arif Wahid ◽

Katy Nugent ◽

Helen Baulch

Keyword(s):

Impedance Spectroscopy ◽

Chlorophyll A ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy

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Study of Reduced Graphene Oxide Dispersions via Electrical Impedance Spectroscopy

Procedia Structural Integrity ◽

10.1016/j.prostr.2020.10.142 ◽

2020 ◽

Vol 28 ◽

pp. 1679-1685

Author(s):

Angeliki-Eirini Dimou ◽

Ioanna Sakellariou ◽

George M. Maistros ◽

Nikolaos D. Alexopoulos

Keyword(s):

Graphene Oxide ◽

Impedance Spectroscopy ◽

Reduced Graphene Oxide ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Reduced Graphene

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Utilization of Electrical Impedance Spectroscopy and Image Classification for Non-Invasive Early Assessment of Meat Freshness

Sensors ◽

10.3390/s21031001 ◽

2021 ◽

Vol 21 (3) ◽

pp. 1001

Author(s):

Sooin Huh ◽

Hye-Jin Kim ◽

Seungah Lee ◽

Jinwoo Cho ◽

Aera Jang ◽

...

Keyword(s):

Impedance Spectroscopy ◽

Prediction Accuracy ◽

Electrical Impedance ◽

Thiobarbituric Acid ◽

Electrical Impedance Spectroscopy ◽

Plate Count ◽

Gradient Boosting ◽

Composition Analysis ◽

Early Assessment ◽

Relative Standard

This study presents a system for assessing the freshness of meat with electrical impedance spectroscopy (EIS) in the frequency range of 125 Hz to 128 kHz combined with an image classifier for non-destructive and low-cost applications. The freshness standard is established by measuring the aerobic plate count (APC), 2-thiobarbituric acid reactive substances (TBARS), and composition analysis (crude fat, crude protein, and moisture) values of the microbiological detection to represent the correlation between EIS and meat freshness. The EIS and images of meat are combined to predict the freshness with the Adaboost classification and gradient boosting regression algorithms. As a result, when the elapsed time of beef storage for 48 h is classified into three classes, the time prediction accuracy is up to 85% compared to prediction accuracy of 56.7% when only images are used without EIS information. Significantly, the relative standard deviation (RSD) of APC and TBARS value predictions with EIS and images datum achieves 0.890 and 0.678, respectively.

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