Activated sludge mixed cultures were cultivated with a glucose containing substrate in order to investigate the relationship between the feeding pattern (continuous or intermittent feeding) and the glycogen reservation capacity of activated sludge. An experimental method to measure the maximum capacity of glycogen reservation in the sludge was developed. Sludge with higher glycogen reservation capacity has an ability to synthesize glycogen faster, which ensures the higher glucose uptake. Therefore, sludge which has high glycogen reservation capacity becomes predominant in intermittently fed reactors. When the feeding pattern was changed from continuous feeding to intermittent feeding, a filamentous bacterium, Type 1701, started to decrease and a gram positive tetrad coccus became predominant. When the feeding pattern was returned to continuous feeding, Type 1701 re-appeared. Type 1701 has lower glycogen reservation capacity than the tetrad coccus. Therefore, the former cannot dominate over the latter in intermittently fed reactors.
