Expression of self-incompatibility and fertility of Brassica napus L. resynthesized by interspecific somatic hybridization

Euphytica ◽  
1992 ◽  
Vol 65 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Richard H. Ozminkowski ◽  
Pablo S. Jourdan
Keyword(s):  
Brassica Napus ◽  
Somatic Hybridization ◽  
Self Incompatibility ◽  
Brassica Napus L ◽  
Interspecific Somatic Hybridization

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

2007 ◽  
Vol 21 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Xingguo Zhang ◽  
Chaozhi Ma ◽  
Tingdong Fu ◽  
Yuanyuan Li ◽  
Tonghua Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Scar Markers ◽  
Self Incompatibility ◽  
Brassica Napus L

scholarly journals Intergeneric Somatic Hybridization Between Brassica napus L. and Sinapis alba L.

2005 ◽  
Vol 47 (1) ◽  
pp. 84-91 ◽  
Author(s):  
You-Ping WANG ◽  
Karin SONNTAG ◽  
Eicke RUDLOFF ◽  
Jian-Min CHEN
Keyword(s):  
Brassica Napus ◽  
Somatic Hybridization ◽  
Sinapis Alba ◽  
Brassica Napus L ◽  
Sinapis Alba L ◽  
Intergeneric Somatic Hybridization

Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.)

1988 ◽  
Vol 75 (4) ◽  
pp. 546-552 ◽  
Author(s):  
C. Primard ◽  
F. Vedel ◽  
C. Mathieu ◽  
G. Pelletier ◽  
A. M. Ch�vre
Keyword(s):  
Brassica Napus ◽  
Somatic Hybridization ◽  
Sinapis Alba ◽  
Brassica Hirta ◽  
Sinapis Alba L ◽  
Interspecific Somatic Hybridization

scholarly journals Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L.

2019 ◽  
Vol 20 (13) ◽  
pp. 3303 ◽  
Author(s):  
Fang Chen ◽  
Yong Yang ◽  
Bing Li ◽  
Zhiquan Liu ◽  
Fawad Khan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Protein Kinase ◽  
Rna Silencing ◽  
Regulatory Mechanism ◽  
Tissue Expression ◽  
Reproductive Organs ◽  
Self Incompatibility ◽  
Phenotypic Analysis ◽  
Brassica Napus L ◽  
Self Compatibility

Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in Brassica napus is not well understood. Here, we report that the M-locus protein kinase (MLPK) BnaMLPKs, the functional homolog of BrMLPKs in Brassica rapa, controls SI in B. napus. We identified four paralogue MLPK genes in B. napus, including BnaA3.MLPK, BnaC3.MLPK, BnaA4.MLPK, and BnaC4.MLPK. Two transcripts of BnaA3.MLPK, BnaA3.MLPKf1 and BnaA3.MLPKf2, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that BnaA3.MLPK, especially BnaA3.MLPKf2, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of BnaMLPKs in B. napus SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of BnaMLPKs in regulating the SI response of B. napus. We found that the expression of SI positive regulators S-locus receptor kinase (SRK) and Arm-Repeat Containing 1 (ARC1) are suppressed in bnmlpk mutant, whereas the self-compatibility (SC) element Glyoxalase I (GLO1) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of MLPK in the SI of B. napus.

Inheritance of self-incompatibility in rutabaga (Brassica napus L. ssp. rapifera (Metzg.) Sinsk.)

1985 ◽  
Vol 27 (6) ◽  
pp. 710-715 ◽  
Author(s):  
R. Ayotte ◽  
P. M. Harney ◽  
B. R. Christie
Keyword(s):  
Brassica Napus ◽  
Microscopic Examination ◽  
Seed Set ◽  
Turnip Mosaic Virus ◽  
Second Line ◽  
Self Incompatibility ◽  
Brassica Napus L ◽  
Microscopic Evaluation ◽  
S Alleles ◽  
S Allele

Self-incompatible and self-compatible lines of Brassica napus L. were crossed and the compatibility status of the F1 and F2 progeny assessed. Compatibility was established by measuring silique and seed set and by microscopic examination of self-pollen behaviour on the stigma and within the style of flowers. Microscopic evaluation was found to be unreliable in determining whether seed set would occur on a plant following self-pollination. The F1 data could not be interpreted because turnip mosaic virus in the greenhouse killed many plants and may have affected the compatibility reaction of the rest. Silique set data for the F2 showed one line (Z) segregated 3:1 self-incompatible to self-compatible, indicating only one locus involved. The 10:6 self-compatible to self-incompatible ratio, obtained for a second line (R) can be explained in the same manner if a dominant modifier (M), which interacts only with S-allele heteozygotes, is also present.Key words: rutabaga, Brassica napus, self-incompatibility, S alleles, genetic.

Progress on characterization of self-incompatibility in Brassica napus L.

Euphytica ◽  
2011 ◽  
Vol 182 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Xingguo Zhang ◽  
Dongmei Yin ◽  
Wei Zhu ◽  
Chaozhi Ma ◽  
Tingdong Fu
Keyword(s):  
Brassica Napus ◽  
Self Incompatibility ◽  
Brassica Napus L
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