scholarly journals Functional Analysis of M-Locus Protein Kinase Revealed a Novel Regulatory Mechanism of Self-Incompatibility in Brassica napus L.

2019 ◽  
Vol 20 (13) ◽  
pp. 3303 ◽  
Author(s):  
Fang Chen ◽  
Yong Yang ◽  
Bing Li ◽  
Zhiquan Liu ◽  
Fawad Khan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Protein Kinase ◽  
Rna Silencing ◽  
Regulatory Mechanism ◽  
Tissue Expression ◽  
Reproductive Organs ◽  
Self Incompatibility ◽  
Phenotypic Analysis ◽  
Brassica Napus L ◽  
Self Compatibility

Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in Brassica napus is not well understood. Here, we report that the M-locus protein kinase (MLPK) BnaMLPKs, the functional homolog of BrMLPKs in Brassica rapa, controls SI in B. napus. We identified four paralogue MLPK genes in B. napus, including BnaA3.MLPK, BnaC3.MLPK, BnaA4.MLPK, and BnaC4.MLPK. Two transcripts of BnaA3.MLPK, BnaA3.MLPKf1 and BnaA3.MLPKf2, were generated by alternative splicing. Tissue expression pattern analysis demonstrated that BnaA3.MLPK, especially BnaA3.MLPKf2, is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of BnaMLPKs in B. napus SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of BnaMLPKs in regulating the SI response of B. napus. We found that the expression of SI positive regulators S-locus receptor kinase (SRK) and Arm-Repeat Containing 1 (ARC1) are suppressed in bnmlpk mutant, whereas the self-compatibility (SC) element Glyoxalase I (GLO1) maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of MLPK in the SI of B. napus.

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

2007 ◽  
Vol 21 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Xingguo Zhang ◽  
Chaozhi Ma ◽  
Tingdong Fu ◽  
Yuanyuan Li ◽  
Tonghua Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Scar Markers ◽  
Self Incompatibility ◽  
Brassica Napus L

Comparative phosphoproteomic analysis of compatible and incompatible pollination in Brassica napus L.

2020 ◽  
Vol 52 (4) ◽  
pp. 446-456 ◽  
Author(s):  
Zhiqiang Duan ◽  
Shengwei Dou ◽  
Zhiquan Liu ◽  
Bing Li ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Protein Phosphorylation ◽  
Rna Degradation ◽  
Fine Tuning ◽  
Brassica Napus L ◽  
Cell Wall Modification ◽  
Incompatible Pollination ◽  
Potential Component ◽  
Self Compatibility ◽  
Plant Pathogen Interactions

Abstract Self-incompatibility (SI) promotes outbreeding and prevents self-fertilization to promote genetic diversity in angiosperms. Several studies have been carried to investigate SI signaling in plants; however, protein phosphorylation and dephosphorylation in the fine-tuning of the SI response remain insufficiently understood. Here, we performed a phosphoproteomic analysis to identify the phosphoproteins in the stigma of self-compatible ‘Westar’ and self-incompatible ‘W-3’ Brassica napus lines. A total of 4109 phosphopeptides representing 1978 unique protein groups were identified. Moreover, 405 and 248 phosphoproteins were significantly changed in response to SI and self-compatibility, respectively. Casein kinase II (CK II) phosphorylation motifs were enriched in self-incompatible response and identified 127 times in 827 dominant SI phosphorylation residues. Functional annotation of the identified phosphoproteins revealed the major roles of these phosphoproteins in plant–pathogen interactions, cell wall modification, mRNA surveillance, RNA degradation, and plant hormone signal transduction. In particular, levels of homolog proteins ABF3, BKI1, BZR2/BSE1, and EIN2 were significantly increased in pistils pollinated with incompatible pollens. Abscisic acid and ethephon treatment partially inhibited seed set, while brassinolide promoted pollen germination and tube growth in SI response. Collectively, our results provided an overview of protein phosphorylation during compatible/incompatible pollination, which may be a potential component of B. napus SI responses.

The self-compatibility mechanism in Brassica napus L. is applicable to F1 hybrid breeding

2011 ◽  
Vol 123 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Takahiro Tochigi ◽  
Hisashi Udagawa ◽  
Feng Li ◽  
Hiroyasu Kitashiba ◽  
Takeshi Nishio
Keyword(s):  
Brassica Napus ◽  
The Self ◽  
Hybrid Breeding ◽  
Brassica Napus L ◽  
F1 Hybrid ◽  
Self Compatibility

The influence of paclobutrazol on the distribution and utilization of 14C-labelled assimilate fixed at anthesis in oil-seed rape (Brassica napus L.)

1985 ◽  
Vol 105 (2) ◽  
pp. 365-373 ◽  
Author(s):  
A. A. Addo-Quaye ◽  
R. W. Daniels ◽  
D. H. Scarisbrick
Keyword(s):  
Brassica Napus ◽  
Reproductive Organs ◽  
Brassica Napus L ◽  
Commercial Practice ◽  
Oil Seed ◽  
Oil Seed Rape ◽  
Stem Reserves

SUMMARYUntreated and paclobutrazol-treated field-grown plants of winter oil-seed rape cv. Jet Neuf were exposed to 14CO2 at anthesis. Redistribution of labelled assimilate was followed in sequential harvests between exposure and maturity.Labelled assimilates moved progressively from the leaves into the stem and reproductive parts. At maturity 9% of 14C fixed at anthesis had been mobilized into reproductive organs. Paclobutrazol increased the proportion of 14C translocated to the uppermost branches especially the terminal raceme.It is suggested that stem reserves are valuable for yields commonly achieved in U.K. commercial practice.

scholarly journals Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2186
Author(s):  
Zhiquan Liu ◽  
Bing Li ◽  
Yong Yang ◽  
Changbin Gao ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Locus ◽  
Recognition System ◽  
Inbred Lines ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

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