5?-Isothiocyanato-N-1-naphthylphthalamic acid: a chemically reactive site-directed irreversible ligand for phytotropin receptors

1993 ◽  
Vol 13 (3) ◽  
pp. 309-316
Author(s):  
Jeffrey C. Suttle ◽  
Fred S. Tanaka ◽  
Ronald G. Wien
Keyword(s):  
Reactive Site ◽  
Naphthylphthalamic Acid ◽  
Irreversible Ligand

Solvent-assisted osmium staining of butyl acrylate and ethylene-propylene-diene in a styrene acrylonitrile matrix

1993 ◽  
Vol 51 ◽  
pp. 900-901 ◽  
Author(s):  
Gudrun A. Hutchins
Keyword(s):  
Butyl Acrylate ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Staining Procedure ◽  
Reactive Site ◽  
Ethylene Propylene ◽  
Minimal Distortion ◽  
Styrene Acrylonitrile ◽  
Engineering Polymers ◽  
Effective Reaction

In order to optimize the toughening effect of elastomers in engineering polymers, it is necessary to characterize the size, morphology and dispersion of the specific elastomer within the polymer matrix. For unsaturated elastomers such as butadiene or isoprene, staining with osmium tetroxide is a well established procedure. The residual carbon-carbon double bond in these materials is the reactive site and forms a 1,2-dilato complex with the OsO4. Incorporation of osmium tetroxide into the elastomer not only produces sufficient contrast for TEM, but also crosslinks the elastomer sufficiently so that ultramicrotomy can be accomplished at room temperature with minimal distortion.Blends containing saturated elastomers such as butyl acrylate (BA) and ethylene propylene diene monomer (EPDM) cannot be stained directly with OsO4 because effective reaction sites such as C=C or -NH2 are not available in sufficient number. If additional reaction sites can be introduced selectively into the elastomer by a chemical reaction or the absorption of a solvent, a modified, two-step osmium staining procedure is possible.

scholarly journals CpG Mutations in the Reactive Site of Human C1¯ Inhibitor

1989 ◽  
Vol 264 (6) ◽  
pp. 3066-3071
Author(s):  
K Skriver ◽  
E Radziejewska ◽  
J A Silbermann ◽  
V H Donaldson ◽  
S C Bock
Keyword(s):  
C1 Inhibitor ◽  
Reactive Site

scholarly journals Reaction of human chymase with reactive site variants of alpha 1-antichymotrypsin. Modulation of inhibitor versus substrate properties.

1993 ◽  
Vol 268 (31) ◽  
pp. 23626-23633 ◽  
Author(s):  
N.M. Schechter ◽  
L.M. Jordan ◽  
A.M. James ◽  
B.S. Cooperman ◽  
Z.M. Wang ◽  
...  
Keyword(s):  
Reactive Site ◽  
Substrate Properties

scholarly journals The temperature-sensitive bond in human alpha 2-macroglobulin is the alkylamine-reactive site.

1980 ◽  
Vol 255 (9) ◽  
pp. 3820-3823
Author(s):  
J.B. Howard ◽  
M. Vermeulen ◽  
R.P. Swenson
Keyword(s):  
Temperature Sensitive ◽  
Reactive Site

scholarly journals Amino acid sequence at the reactive site of human alpha 1-antichymotrypsin.

1983 ◽  
Vol 258 (21) ◽  
pp. 12749-12752 ◽  
Author(s):  
M Morii ◽  
J Travis
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Reactive Site

scholarly journals Combinatorial mutagenesis of the reactive site region in plasminogen activator inhibitor I

1991 ◽  
Vol 266 (13) ◽  
pp. 8495-8500
Author(s):  
J.D. York ◽  
P. Li ◽  
S.J. Gardell
Keyword(s):  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Reactive Site ◽  
Combinatorial Mutagenesis ◽  
Activator Inhibitor

scholarly journals In vitro and in vivo evidence for actin association of the naphthylphthalamic acid-binding protein from zucchini hypocotyls

1998 ◽  
Vol 13 (3) ◽  
pp. 291-301 ◽  
Author(s):  
John H. Butler ◽  
Shiquan Hu ◽  
Shari R. Brady ◽  
Michael W. Dixon ◽  
Gloria K. Muday
Keyword(s):  
Binding Protein ◽  
Acid Binding Protein ◽  
Naphthylphthalamic Acid
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